Most Down Articles

Published in last 1 year | In last 2 years| In last 3 years| All| Most Downloaded in Recent Month | Most Downloaded in Recent Year|

In last 2 years

Please wait a minute...


For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails

Select

Evaluation of red and white aesthetic effects of porcelain veneers in the restoration of developmental anterior dental gaps DENG Xiao-lin, YUAN Shuang, ZHU Dan-ting, SHI Le Shanghai Journal of Stomatology    2023, 32 (3): 276-279.   DOI: 10.19439/j.sjos.2023.03.011 Abstract （226）      PDF （873KB）（266）       Knowledge map    PURPOSE: To evaluate the red and white aesthetic effect of porcelain veneer in the restoration of developmental anterior interdental spaces. METHODS: A total of 152 anterior teeth in 64 patients with developmental anterior dental gaps were restored using porcelain veneers, the aesthetic effects before and after restoration were evaluated by pink aesthetic index (PES) and white aesthetic index(WES), the aesthetic effect of gingival papilla filling and reconstruction was evaluated by interdental gingival papilla index (PIS), and visual analogue score (VAS) was used to compare the satisfaction of patients before and after restoration. SPSS 203.0 software package was used for statistical analysis. RESULTS: The overall mean scores of PES before and after restoration of 152 developmental anterior interdental teeth were 9.63±2.23 and 13.64±0.88, respectively. The average scores of WES before and after restoration were 6.85±1.87 and 9.81±0.58, respectively. There were significant differences of PES and WES scores before and after restoration(P<0.01). According to the requirements of "near" perfect restoration(PES≥13 points, WES≥9 points), the red and white aesthetic effect after restoration was near the standard. The scores of PIS before and after restoration were 1.86±0.67 and 2.97±0.18, the interdental gingival papilla was completely filled with space, and the shape was ideal, there were significant statistical differences before and after restoration(P<0.01). Patients were more satisfied with the smile curve and morphology of the restored anterior teeth than other parameters. CONCLUSIONS: The aesthetic effect of using porcelain veneers to repair developmental anterior interdental gaps is ideal, among which the filling of the papillae between the teeth can meet the expectations of patients, and the aesthetic effect evaluation of PIS and PES/WES applied to porcelain veneers to repair developmental anterior interdental spaces has practical guiding significance in clinical practice. Reference | Related Articles | Metrics

Select

A reflectional insight into orthodontic theoretical renovation and technological innovation SHEN Gang Shanghai Journal of Stomatology    2023, 32 (2): 113-119.   DOI: 10.19439/j.sjos.2023.02.001 Abstract （249）      PDF （867KB）（254）       Knowledge map    Orthodontics, among other specialties in dentistry, remains most dynamic and vigorous in upgrading and reforming its fundamental doctrines and clinical technologies. Orthodontic specialty in China has played a leading role in recent years in reshaping fundamental theories and in creating cutting-edge therapeutic modalities. The newly developed diagnostic classification system, a complementary addition to that of Angle's, not only defines the natures, but also identifies the developmental mechanisms of malocclusions. Orthopedic therapy aiming to relocate the mandible before correcting the dentition is emerging as an indispensable treatment mode to malocclusions concurring with mandibular deviation. Whilst correlations exist between malocclusion and TMD susceptibility and incidence, the specifically designed orthopedic and orthodontic approaches have proven effective in managing cases inflicted by TMD conditions. Innovative efforts that create the GS products, have redefined the clear appliances much more beyond merely clear aligners, thus further pushing the boundaries of both the indications and clinical use for clear appliances. Reference | Related Articles | Metrics

Select

Analysis of potential pathogenic factors of trigeminal neuralgia in rats LIU Yue-min, CHAI Ying, WEI Wen-bin, LIU Zhi-yang, HAN Zi-xiang, CHEN Min-jie Shanghai Journal of Stomatology    2023, 32 (1): 33-39.   DOI: 10.19439/j.sjos.2023.01.007 Abstract （176）      PDF （1559KB）（236）       Knowledge map    PURPOSES: Transcriptomics-based analysis of key transcriptional molecules in the pathogenesis of trigeminal neuropathic pain was conducted to screen key molecules in the pathogenesis of trigeminal neuralgia. METHODS: Rat trigeminal nerve pathological pain model, namely chronic constriction injury of distal infraorbital nerve (IoN-CCI), was constructed and animal behaviors postsurgery were observed and analyzed. Trigeminal ganglia were collected for RNA-seq transcriptomics analysis. StringTie was used to annotate and quantify genome expression. DESeq2 was applied to compare between groups with P value less than 0.05 and fold change greater than 2 times and less than 0.5 times to screen differential genes, and display them with volcano graphs and cluster graphs. ClusterProfiler software was used to perform GO function enrichment analysis of differential genes. RESULTS: On the fifth postoperative day (POD5), the rat's face-grooming behavior increased to a peak; on the seventh postoperative day (POD7), the von-frey value dropped to the lowest value, indicating that the mechanical pain threshold of rats was significantly decreased. RNA-seq analysis of IoN-CCI rat ganglia found that the significantly up-regulated signaling pathways included B cell receptor signaling pathway, cell adhesion, complement and coagulation cascade pathways; significantly down-regulated pathways were related to systemic lupus erythematosus. Multiple genes among Cacna1s, Cox8b, My1, Ckm, Mylpf, Myoz1, Tnnc2 were involved in mediating the occurrence of trigeminal neuralgia. CONCLUSIONS: B cell receptor signaling pathway, cell adhesion, complement and coagulation cascade pathways, neuroimmune pathways are closely related to the occurrence of trigeminal neuralgia. The interaction of multiple genes among Cacna1s, Cox8b, My11, Ckm, Mylpf, Myoz1, Tnnc2 leads to the occurrence of trigeminal neuralgia. Reference | Related Articles | Metrics

Select

Study on the effect of alginate impression spray disinfection process on model accuracy ZHANG Jia-yuan, YU De-dong, YE Li-juan, WU Chun-lan, LIU Jing Shanghai Journal of Stomatology    2023, 32 (5): 449-454.   DOI: 10.19439/j.sjos.2023.05.001 Abstract （217）      PDF （1125KB）（209）       Knowledge map    PURPOSE: To investigate the effect of a new cleaning and disinfection process of oral impressions on the accuracy of alginate dental impression. METHODS: Sixteen young volunteers were selected to make alginate oral impression (2 times for each upper and lower jaw) and oral scandata acquisition (1 time for each upper and lower jaw) to obtain STL data. The 2 pairs of alginate impressions of each subject were numbered group 1, group 2, inwhich group 1 was not sterilized and the oral impression film in group 2 was treated with the method and steps of new oral impression cleaning and disinfection. All the oral impressions of the subjects were perfused with plaster models in the same standard way and demoulded. The obtained plaster models were examined and scanned after appropriate trimming and grinding. The plaster model data obtained from each group of each patient after warehouse scanning were compared with the orally scanned model data for alignment analysis, and root mean square(RMS) was used as a parameter to evaluate the deviation between the test model and the standard model. The chromatograms of the deviations were saved for visualization and analysis to observe the differences in model accuracy from several dimensions. Statistical analysis was performed with SPSS 23.0 software package. RESULTS: The differences in RMS of model accuracy of maxillary dental series of alginate impressions were statistically significant, in which the RMS of the group disinfecting the maxillary oral impression film with a new oral impression cleaning and disinfection process was significantly higher than that of the nonsterilized group (P=0.006), while the difference between model accuracy RMS of the mandibular disinfection group and non-disinfection group was not statistically significant(P=0.874). CONCLUSIONS: The new technical specifications for cleaning and disinfection of dental impressions recommended by the Chinese Stomatological Association have certain effects on the accuracy of dental impressions made of alginate material,and the details in its process should be further improved. Reference | Related Articles | Metrics

Select

A case of two-stage orthodontic treatment of skeletal ClassⅡmalocclusion with mandibular retrusion and maxillary dental arch stenosis ZHANG Yun, LI Xiao-bing Shanghai Journal of Stomatology    2023, 32 (5): 555-560.   DOI: 10.19439/j.sjos.2023.05.020 Abstract （266）      PDF （2656KB）（200）       Knowledge map    This article reviewed two-stage orthodontic treatment of a patient with skeletal ClassⅡmalocclusion with mandibular retrusion and maxillary dental arch stenosis. The patient was also accompanied by premature loss of bilateral mandibular deciduous canines and mesialization of mandibular posterior teeth. The first-stage treatment mainly corrected the maxillary dental arch stenosis, maxillary anterior teeth protrusion, mandibular retrusion and skeletal ClassⅡmalocclusion, while the second-stage treatment mainly corrected the mesialization of mandibular posterior teeth and alignment of maxillary and mandibular dentition. The two-stage orthodontic treatment finally enabled the patient to achieve good dental arch shape, tooth alignment, skeletal face pattern and soft tissue profile. Reference | Related Articles | Metrics

Select

Effects of Fusobacterium nucleus derived outer membrane vesicles on claudin-4 expression in oral epithelial cells YANG Cheng-long, YANG Meng, WANG Yu-feng, SONG Chen-cheng, DU Guan-huan, TANG Guo-yao Shanghai Journal of Stomatology    2023, 32 (2): 126-131.   DOI: 10.19439/j.sjos.2023.02.003 Abstract （208）      PDF （1295KB）（193）       Knowledge map    PURPOSE: To investigate the effect of outer membrane vesicles (OMVs) secreted by Fusobacterium nucleatum (F.n) on Claudin-4 of human oral keratinocytes (HOK) and oral epithelial barrier function. METHODS: Fusobacterium nucleatum was cultured under anaerobic conditions. The OMVs were extracted by dialysis and characterized by nanosight and transmission electron microscopy (TEM). HOK were stimulated with OMVs at different mass concentrations(0-100 μg/mL) for 12 h, and stimulated with 100 μg/mL OMVs for 6 h and 12 h respectively. The expression of Claudin-4 at gene and protein level was analyzed by RT-qPCR and Western blotting. Inverted fluorescence microscope was used to observe co-localization of HOK and OMVs and localization and distribution of Claudin-4 protein. Human oral epithelial barrier was constructed by Transwell apical chamber. Transepithelial electrical resistance(TER) of barrier was measured with a transmembrane resistance measuring instrument(EVOM2), and the permeability of the barrier was evaluated by transmittance of fluorescein isothiocyanate-dextran(FD-4). Statistical analysis was performed with GraphPad Prism 8.0 software package. RESULTS: Compared with the control group, the expression of Claudin-4 at protein and gene level in the HOK of OMVs stimulated group was significantly reduced (P<0.05), and immunofluorescence showed that the continuity of Claudin-4 fluorescence among cells was destroyed. OMVs stimulation decreased TER value of oral epithelial barrier(P<0.05) and increased the transmittance of FD-4(P<0.05). CONCLUSIONS: OMVs derived from Fusobacterium nucleatum may damage oral mucosal epithelial barrier function through inhibiting the expression of Claudin-4. Reference | Related Articles | Metrics

Select

Clinical effect of simultaneous implantation and maxillary sinus floor elevation with or without bone graft HU Ning, LI Feng-dan, JIANG Yin-hua Shanghai Journal of Stomatology    2023, 32 (1): 101-104.   DOI: 10.19439/j.sjos.2023.01.019 Abstract （165）      PDF （448KB）（187）       Knowledge map    PURPOSE: To investigate the difference of osteogenic effect and stability of maxillary sinus implants 6 months after maxillary sinus elevation with or without bone grafting. METHODS: A total of 150 patients with maxillary sinus floor lift and simultaneous implantation in Lishui People's Hospital from December 2019 to December 2021 were divided into group A (internal maxillary sinus lift + bone grafting) and group B (internal lift without bone grafting). Preoperative and postoperative CBCT data and implant stability of all patients were measured and analyzed to compare whether there were differences in clinical efficacy between the two groups. SPSS 25.0 software package was used for data analysis. RESULTS: A total of 199 implants were implanted, the implant retention rate was 97.6% in group A and 95.7% in group B one year after surgery, with no significant difference between the two groups(P>0.05). There was no significant difference in residual bone height(RBH) and gray scale value (HU) between the two groups before and 6 months after operation (P>0.05). There was no significant difference in ISQ value between the two groups during operation and 6 months after operation(P>0.05). CONCLUSIONS: In cases with the remaining alveolar bone height of 3~8 mm and the planned lifting height of 3~4 mm, maxillary sinus floor lifting procedure achieved good clinical results in both groups with or without bone grafting, which indicated that bone grafting had little effect on retention rate and stability of the implants. Reference | Related Articles | Metrics

Select

Application of 3D printing modified tooth-supported cyst plug in decompression of mandibular cystic lesions KOU Peng, LI Ying-mei, LI Zhi-ping, MENG Jian Shanghai Journal of Stomatology    2023, 32 (2): 203-208.   DOI: 10.19439/j.sjos.2023.02.017 Abstract （253）      PDF （1849KB）（182）       Knowledge map    PURPOSE: To evaluate the application value of 3D printing modified dental support cyst plug in fenestration of large jaw cystic lesions. METHODS: Forty patients with mandibular cystic disease in Xuzhou Central Hospital from October 2019 to April 2021 were selected. They were randomly divided into experimental group(3D printing group) and control group (traditional plug group), with 20 cases in each group. All enrolled patients underwent preoperative digital modeling of cystic lesions of the jaw, obtained the cystic cavity volume data of preoperative lesions, designed the windowing site according to the plan and performed decompression for jaw cysts. Three days after surgery, the patient's postoperative CBCT and Oral-scan data in the experimental group was obtained, and a digitally modified tooth-supported cyst plug with porous column channel was designed, and titanium alloy material for 3D printing was selected. In the control group, the plug was manually molded by experienced physicians. The visual analogue scale(VAS) score of pain, retention, mechanical properties of the plug and its effect on the adjacent teeth were compared between the two groups during the process of model preparation, and the changes of the cyst volume 1, 3 and 6 months after operation were compared between the two groups. SPSS 25.0 software package was used for data analysis. RESULTS: Compared with the control group, the patients in the experimental group who made titanium alloy as printing material by digital impression complained more comfortable, and the mechanical strength and stability of the cyst plug were better than those in the control group(P< 0.05). There was no significant difference in retention between the two groups(P>0.05). The reduction rate of cyst volume in the experimental group was significantly higher than that in the traditional plug group 3 and 6 months after operation(P<0.05). CONCLUSIONS: The modified tooth-supported titanium alloy cyst plug with digital 3D printing has good mechanical properties and stability. It has little damage to the abutment and no lateral force, and has the advantages of precision, individualization and comfort. The improved irrigation and injection channel can fully flush the cavity and speed up the narrowing of the cyst and reduce the waiting time before the second operation, which is worth promoting in clinical practice. Reference | Related Articles | Metrics

Select

Effect of three mixing methods on air bubbles and solidification state of alginate impression materials ZHOU Yi-an, XIAO Song, XIE Xue-mei, WANG Yu-hua Shanghai Journal of Stomatology    2023, 32 (1): 12-16.   DOI: 10.19439/j.sjos.2023.01.003 Abstract （530）      PDF （658KB）（179）       Knowledge map    PURPOSE: To evaluate the effect of full-automatic mixing machine method, clockwise manual mixing and combined eight-shaped manual mixing on air bubble content, flowability, temperature, working time and setting time of alginate impression materials. METHODS: With the same condition, alginate impression materials were mixed by three different methods. The number of bubbles, area, flowability, temperature, working time and setting time were evaluated with SPSS 24.0 software package. RESULTS: The number of bubbles in the automatic mixing group was (2.30±2.50), and the area was (0.17±0.18) mm2, which was less than the number of clockwise manual mixing group (59.60±14.19), and the total area (7.41±2.24) mm2 (P<0.01). The flowability of the clockwise manual mixing group [(39.52±0.85) mm] was less than that of the full-automatic mixing group [(50.78±0.90) mm] and the combined eight-character manual mixing group [(50.36±1.75) mm](P<0.01).The setting time of the material mixed by three methods was eligible for clinical use. CONCLUSIONS: The mixing method of alginate impression material has an effect on material's bubble content, flowability and temperature changes. The impression materials mixed by full-automatic mixing method are better in terms of bubble content, flowability and other properties. If manual mixing is used, combined eight-shaped manual mixing method can help reduce impression bubbles and deformation, and improve flowability. Reference | Related Articles | Metrics

Select

Comparison of clear aligners and customized lingual appliance for bimaxillary dentoalveolar protrusion: a retrospective study LI Zhen-xia, YUAN Ling-jun, ZHENG Xiao-wen, LIU Lu, LIU Chao, FANG Bing Shanghai Journal of Stomatology    2023, 32 (5): 480-484.   DOI: 10.19439/j.sjos.2023.05.006 Abstract （164）      PDF （629KB）（176）       Knowledge map    PURPOSE: To compare the treatment effects of clear aligners and customized lingual appliance on treating bimaxillary dentoalveolar protrusion patients with first premolar extractions. METHODS: Fifty-four patients with bimaxillary dentoalveolar protrusion treated in Shanghai Ninth People Hospital were involved in the retrospective study. Twenty-five cases used clear aligners and 29 cases used customized lingual appliance. All of them were treated by extracting 4 first premolars and retracting anterior teeth with strong anchorage. The changes of anterior tooth and soft tissue adduction before and after treatment were compared by lateral cephalometric measurements. SPSS 26.0 software package was used for data analysis. RESULTS: The total course of treatment in the clear aligners group (46.32±7.37 months) was about 10.8 months longer than that in the customized lingual appliance group (35.55±5.90 months) (P<0.05). There was no significant difference in upper incisor retraction, lower incisor inclination and overjet reduction between the two groups(P>0.05). There were significant differences in upper lip retraction, lower lip retraction, upper incisor torque reduction, and overbite reduction between the two groups(P<0.05). Customized lingual appliance group showed a significant improvement of lips retraction and overbite reduction in orthodontic treatment. For the correction of overjet, there was no significant difference between the two groups (P=0.337). The data of U1-OP (the distance between edge of the upper central incisor and the functional occlusal plane) was not in normal distribution, and there was no significant difference between the two groups(P=0.184). CONCLUSIONS: The two techniques can both retract the anterior teeth and lips to improve the profile. However, the customized lingual appliance was more effective in improving the soft tissue profile of patients with bimaxillary dentoalveolar protrusion, with shorter treatment course. Reference | Related Articles | Metrics

Select

Effect of polishing method and time on the fitness of CAD/CAM zirconia ceramic crown ZHAO Chen, BAI Yue-hui, LIU Yang, JIANG Shan-shan, SHANG Qing-long, ZHAO Tian-tian, YUAN Shuo, LI Ning, JIAO Jian-ping Shanghai Journal of Stomatology    2023, 32 (3): 236-240.   DOI: 10.19439/j.sjos.2023.03.003 Abstract （329）      PDF （870KB）（174）       Knowledge map    PURPOSE: To compare the effect of different polishing methods and time treatment on the fitness of CAD/CAM zirconia ceramic crowns. METHODS: Sixteen intact maxillary premolars were randomly divided into two groups, group A was treated with silicon carbide burs, while group B was treated with tungsten steel burs. At different polishing time points of the same tooth, digital impressions of each group were obtained, which were used to manufacture CAD/CAM zirconium ceramic crowns. After trial fitting, the gap impressions were obtained by using silicone rubber replication method, and the marginal and internal discrepancies were assessed. The data were statistically analyzed with SPSS 21.0 software package. RESULTS: The difference between the gap values of the marginal and internal markers of group A and group B was not statistically significant(P>0.05). Compared with the no-polishing process, the differences of the marginal gap (39.67±8.35) μm and internal gap (45.18±7.16) μm of group A polished for 4 min, and the marginal gap (51.25±14.73) μm, and internal gap (48.56±6.45) μm of group B polished for 3 min, as well as the marginal gap (48.87±8.90) μm, and internal gap (45.99±7.12) μm of group B polished for 4 min, were all significant(P<0.05). CONCLUSIONS: CAD/CAM zirconia ceramic crowns treated with silicon carbide bur for polishing 4 min and tungsten steel for 3 min has the best fitness. Reference | Related Articles | Metrics

Select

Imaging findings of carcinoma ex pleomorphic adenoma in minor salivary glands ZHU Wen-Jing, YU Qiang, TAO Xiao-feng, WANG Shao-ying, TIAN Tao, ZHANG Chun-ye Shanghai Journal of Stomatology    2023, 32 (3): 255-260.   DOI: 10.19439/j.sjos.2023.03.007 Abstract （303）      PDF （1392KB）（173）       Knowledge map    PURPOSE: To summarize the CT and MR imaging features of carcinoma ex pleomorphic adenoma(Ca-ex-PA) in minor salivary gland, and analyze the correlation between various features and pathological classification. METHODS: Forty-three patients with Ca-ex-PA in minor salivary gland were collected. The CT and MRI findings were retrospectively analyzed and correlated with their pathological types. Fisher's exact test was used to analyze the correlation between various imaging features (tumor morphology, boundary, internal structure, bone invasion, cervical lymph node metastasis) and pathological types with SPSS 25.0 software package. RESULTS: Among the 43 patients with Ca-ex-PA, 83.7%(36/43) of the tumors were lobulated; 81.4%(35/43) showed cystic degeneration or necrosis, with heterogeneous enhancement. Coarse calcification or mixed calcification was found in 37.2%(16/43), 25.6%(11/43) had compressive absorption of adjacent bone. 75%(12/16) of type Ⅰ/Ⅱ tumors had regular morphology (round or oval), and 77.8%(21/27) of type Ⅲ tumors had irregular morphology, 93.8%(15/16) of type Ⅰ/Ⅱ tumors had well-defined margin and 66.7%(18/27) of type Ⅲ tumors had ill-defined margin. Osteolytic bone resorption occurred in 59.3%(16/27) of type Ⅲ tumors. The average maximum diameter of type Ⅰ/Ⅱ tumors was significantly shorter than that of type Ⅲ(P<0.05). Fisher's exact test showed the characteristics of tumor morphology, boundary and osteolytic bone resorption were related to pathological grouping(P<0.001). CONCLUSIONS: Most Ca-ex-PA in minor salivary glands is characterized by lobular and heterogeneous enhanced neoplasm on CT and MR imaging. A round or oval tumor with well-defined margin usually correlates with typeⅠ and Ⅱ, contrarily, an irregular mass with ill-defined margin and osteolytic bone destruction usually correlates with type Ⅲ. Combining the three characteristics of morphology, boundary and osteolysis is more helpful to distinguish type Ⅰ/Ⅱ and type Ⅲ tumors. Reference | Related Articles | Metrics

Select

Comparison of clinical effects of different irrigant solutions combined with different agitation techniques in the treatment of chronic apical periodontitis with fistula LIU Hui-nan, ZHANG Fu-hua, PU Zhuo-guang Shanghai Journal of Stomatology    2023, 32 (2): 188-192.   DOI: 10.19439/j.sjos.2023.02.014 Abstract （223）      PDF （625KB）（171）       Knowledge map    PURPOSE: To evaluate the effect of combination of auxiliary irrigation technology and root canal irrigation solution in the treatment of chronic apical periodontitis with fistula, and try to seek a more effective and minimally invasive therapeutic strategy. METHODS: One hundred and fifty patients with fistulous chronic apical periodontitis who were diagnosed in Hefei Stomatological Hospital from January 2021 to January 2022 were randomly divided into 6 groups, 25 cases in each group. The 6 groups were as follows, group A: 0.5%NaOCl +ultrasonically activated irrigation; Group B: 1.0%NaOCl+ultrasonically activated irrigation; Group C: 2.0%CHX+ultrasonically activated irrigation; Group D: 0.5%NaOCl+sonic activation; Group E: 1.0%NaOCl+sonic activation; Group F: 2.0%CHX+sonic activation. The fistula healing time, treatment effect and postoperative pain were observed in each group. The data were analyzed with SPSS 20.0 software package. RESULTS: In terms of fistula healing, the 10-day fistula healing rate of group E and group F was higher than that of group A and group D,and the difference was statistically significant(P<0.05); but there was no significant difference between group E and group F (P>0.05). The effective rate at 1 month after operation in group A was lower, and the difference was significant (P<0.05). In terms of postoperative pain, the VAS score of group A was lower than that of group E and group F at all time points, and the difference was statistically significant(P<0.05). CONCLUSIONS: In the treatment of chronic apical periodontitis with fistula, 1.0% NaOCl or 2.0% CHX combined with ultrasonically activated irrigation or sonic activation obtain a better short-term effect,of which the sonic activation group can also obtain early healing of the fistula, but the incidence of postoperative pain is higher when sonic activation is used. Reference | Related Articles | Metrics

Select

Comparison of dentinal microcracks caused by new generation of single reciprocating files after root canal preparation ZHANG Bin, JIA Ming-yu, FAN Rui, TIAN Jian-gang, GE Xue-jun, HUANG Rui-zhe Shanghai Journal of Stomatology    2023, 32 (1): 1-5.   DOI: 10.19439/j.sjos.2023.01.001 Abstract （232）      PDF （773KB）（169）       Knowledge map    PURPOSE: To compare the incidence of dentinal microcracks after root canal preparation by new generation of nickel-titanium instrument WaveOne Gold, Reciproc Blue with previous WaveOne and Reciproc. METHODS: Ninety extracted single-rooted mandibular premolars were randomly divided into 6 groups(n=15). The root canals were instrumented by using Hand K files, WaveOne, Reciproc, WaveOne Gold and Reciproc Blue. Fifteen teeth were left unprepared and served as negative controls. The root canals were all prepared to 25#. The roots were then sectioned at 3 mm, 6 mm and 9 mm from the apical orifice using a hard tissue slicer. The slices were observed under stereoscopic microscope at ×25 magnification. SPSS 17.0 software package was used for statistical analysis. RESULTS: No dentinal microcrack was found in the hand K files group and negative control group. The reciprocating single files WaveOne, WaveOne Gold, Reciproc and Reciproc Blue all produced dentinal microcracks after root canal preparation. The WaveOne generated the most dentinal microcracks than the hand K files(P<0.05), and the microcracks were mainly concentrated in the middle part of the root. The number of dentinal microcracks caused by Reciproc and Reciproc Blue was the same, with no significant difference(P>0.05). CONCLUSIONS: The new generation of reciprocating files of WaveOne Gold and Reciproc Blue may not increase the incidence of dentinal microcracks after root canal preparation. Reference | Related Articles | Metrics

Select

Effect of collagen sponge on the healing process of alveolar fossa after tooth extraction in rats LI Jun-fu, ZHU Jia, JIANG Juan, WANG Xu-xia, CHEN Dai-yun, ZHAO Zuo-qin Shanghai Journal of Stomatology    2023, 32 (3): 241-245.   DOI: 10.19439/j.sjos.2023.03.004 Abstract （342）      PDF （759KB）（169）       Knowledge map    PURPOSE: To investigate the effect of collagen sponge on early bone healing process of alveolar fossa after tooth extraction in rats. METHODS: A total of 16 healthy female SD rats were selected. Animal models with tooth extraction were established. The right alveolar fossa inserted with collagen sponge was as the experimental group, and the left alveolar fossa was as the control group with treatment. The rats were sacrificed 1, 2, 4 and 8 weeks after tooth extraction, and the osteogenesis of alveolar fossa was observed. Real-time quantitative PCR (qt-PCR) was used to detect the changes of osteogenesis related gene expression. SPSS 19.0 software package was used for statistical analysis. RESULTS: After surgery, alveolar cavity healing was significantly better in the experimental group than in the control group. Osterix, Runx2 and Vegf genes were expressed in the experimental group and the control group, and the expression levels of related genes in the experimental group were significantly higher than the control group 1, 2, 4 and 8 weeks after surgery(P<0.05). CONCLUSIONS: Collagen sponge could promote early alveolar bone healing, possibly related to the expression level of osteogenic genes regulated by collagen sponge. Reference | Related Articles | Metrics

Select

Evaluation of low intensity Nd: YAG laser and traditional drugs in the treatment of myofascial pain LI Xu, ZHAO Ning, LIU Zong-lin, ZHANG Shan-yong, CAI Zhen, ZHU Chao Shanghai Journal of Stomatology    2023, 32 (3): 261-265.   DOI: 10.19439/j.sjos.2023.03.008 Abstract （194）      PDF （537KB）（166）       Knowledge map    PURPOSE: To evaluate the efficacy of low intensity Nd: YAG laser and traditional drugs in the treatment of myofascial pain (MP). METHODS: Eighty patients with MP were divided into laser group(n=40) and traditional medicine group(n=40) according to the principle of randomization and double-blindness. The patients in the laser group were treated with low intensity Nd :YAG laser(1 064 nm, 8 J/cm2, 250 mW) , with an interval of 48 h between the two laser treatments. The whole course of treatment was 10 times. Patients in the traditional medicine group uesd celecoxib capsules, 1 capsulet each time(0.2 g), twice a day for 2 weeks. Before and after each treatment, mouth opening, protrusion excursion, lateral movement of the affected side and lateral movement of the contralateral side were measured, and pain visual analogue scores (VAS) were measured and recorded. The data were statistically analyzed with SPSS 22.0 software package. RESULTS: Patients in laser group had significantly improved mandibular function and movement status(P<0.05) and pain symptoms(P<0.05); patients in traditional medicine group had the same significant improvement on mandibular functional movement status(P<0.05) and pain symptoms (P<0.05). The total effective rate of the two groups had no significant difference(P>0.05). The VAS score of patients in laser group was lower than that of traditional medicine group, but the difference was not significant(P>0.05). CONCLUSIONS: Low intensity Nd: YAG laser and traditional medicine can effectively relieve the symptoms of myofascial pain and improve mandibular function and movement. Laser treatment has the advantages of short course of treatment, high efficiency, no pain and fewer side effects, which is worthy of clinical application. Reference | Related Articles | Metrics

Select

Application of digital dynamic smile aesthetic simulation teaching method in orthodontic practicum SU Xiao-xia, ZHOU Li, LIAO Li, LI Yue-yan, LI Xiao-bin Shanghai Journal of Stomatology    2023, 32 (2): 220-224.   DOI: 10.19439/j.sjos.2023.02.020 Abstract （179）      PDF （1796KB）（164）       Knowledge map    PURPOSE: To integrate digital dynamic smile aesthetic simulation (DSAS) cognitive education in orthodontic practicum and evaluate the teaching effects. METHODS: A total of 32 dental students during orthodontic practicum were randomly divided into two groups. One group received traditional teaching method to draft treatment plan, and another group was implemented with DSAS teaching method. Then two groups exchanged. Students were asked to grade both teaching methods and statistical analysis was performed on the scoring results with SPSS 24.0 software package. RESULTS: The scores of the DSAS teaching method was much higher than traditional method, and the difference was statistically significant(P=0.012). Students considered that DSAS teaching method was more “novel and fascinating”, and also “convenient for comprehending of orthodontic treatment”. Students hoped to popularize the DSAS teaching method in future orthodontic practicum. CONCLUSIONS: As a novel teaching method, DSAS is more intuitive and vivid to stimulate students' interest in learning, and it is helpful to improve the effect of orthodontic practical teaching. Reference | Related Articles | Metrics

Select

Morphological changes of upper airway in patients with skeletal Class Ⅲ malocclusion after bimaxillary surgery and correlation analysis YAN Jie-ya, LIN Yi-jun, LI Ya-xin, LAO An, LIAO Sheng-hsuan, LIU Jia-qiang Shanghai Journal of Stomatology    2023, 32 (5): 485-490.   DOI: 10.19439/j.sjos.2023.05.007 Abstract （193）      PDF （952KB）（160）       Knowledge map    PURPOSE: To analyze the morphological changes of the upper airway and related influencing factors in patients with skeletal Class Ⅲ malocclusion after bimaxillary surgery. METHODS: Twenty skeletal Class Ⅲ patients who underwent Le Fort I osteotomy and bilateral sagittal split ramus osteotomy(BSSRO) for maxillary advancement and mandibular setback were selected. The patients received CT scans before(T0) and 3-6 months after surgery, and the images were reconstructed three-dimensionally with Dolphin Imaging 11.9 software. Changes in the volume, cross-sectional area, and landmarks of each soft and hard tissue of the airway were measured. Statistical analysis of the data was performed using SPSS 25.0 software package. RESULTS: The volume of nasopharyngeal airway increased after operation (P<0.05), and the volume of oropharyngeal airway decreased significantly(P<0.01). The cross-sectional area of the airway at the plane of the second cervical vertebra was significantly decreased (P<0.01), and the coronal and sagittal diameters were decreased(P<0.05). The change of nasopharyngeal airway volume was moderately positively correlated with the sagittal change of the posterior nasal spine (r=0.460, P<0.05), and the change of oropharynx and laryngopharyngeal airway volume was positively correlated with the vertical change of the midpoint of the soft palate(r=0.496, 0.696, P<0.05). The airway cross-sectional area in the second and third cervical vertebra planes and the sagittal diameter of the airway in the third cervical vertebra plane were positively correlated with the vertical changes of the midpoint of the soft palate(r=0.474, 0.629, 0.547, P<0.05). The change of airway cross-sectional area at the third cervical vertebra plane was moderately negatively correlated with the change of mandibular plane angle(r=-0.536, P<0.05). CONCLUSIONS: The volume and cross-sectional area of oropharyngeal airway in skeletal Class Ⅲ patients after bimaxillary surgery will decrease. However, the total upper airway volume doesn't change significantly. The changes in the upper airway are correlated with the changes in some soft and hard tissue landmarks. Reference | Related Articles | Metrics

Select

Role and mechanism of miR-497-5p in the differentiation and mineralization of pre-osteoblast MC3T3-E1 HU Xi, LUO Jun Shanghai Journal of Stomatology    2023, 32 (1): 17-22.   DOI: 10.19439/j.sjos.2023.01.004 Abstract （171）      PDF （970KB）（159）       Knowledge map    PURPOSE: To study the role of microRNA (miR)-497-5p in the differentiation and mineralization of pre-osteoblasts MC3T3-E1, and to explore the related mechanisms. METHODS: The third generation MC3T3-E1 cells were transfected into the miR-497-5p overexpression plasmid miR-497-5p mimics, the low expression plasmid miR-497-5p inhibitor, and the negative control plasmid miR-497-5p NC. They were set up as the miR-497-5p mimics group, miR-497-5p inhibitor group, and miR-497-5p NC group. The cells untreated was set up as the blank group. Fourteen days after osteogenic induction, alkaline phosphatase (ALP) activity was detected. The expression of osteocalcin (OCN) and type I collagen (COL-I) proteins related to osteogenic differentiation were detected by Western blotting. Mineralization was observed by alizarin red staining method. The expression of Smad ubiquitination regulatory factor 2 (Smurf2) protein was detected by Western blotting. The targeting relationship between miR-497-5p and Smurf2 was verified by dual luciferase experiment. Statistical analysis was performed by SPSS 25.0 software package. RESULTS: Compared with the blank group and miR-497-5p NC group, ALP activity of the miR-497-5p mimics group was enhanced, the expression of OCN, COL-I protein and the ratio of the area of mineralized nodules was increased, and the expression of Smurf2 protein was decreased(P<0.05). ALP activity of the miR-497-5p inhibitor group was weakened, the expression of OCN, COL-I protein and the ratio of the area of mineralized nodules was decreased, and the expression of Smurf2 protein was increased(P<0.05). Compared with Smurf2 3'-UTR-WT+miR-497-5p NC group, Smurf2 3'-UTR-MT+miR-497-5p mimics group, Smurf2 3'-UTR-MT+miR-497-5p NC group, the activity of dual luciferase in the WT+miR-497-5p mimics group was decreased (P<0.05). CONCLUSIONS: Overexpression of miR-497-5p can promote the differentiation and mineralization of pre-osteoblasts MC3T3-E1, and its mechanism may be related to the negatively targeted regulation of Smurf2 protein expression. Reference | Related Articles | Metrics

Select

Study on promoting bone formation in osteoporotic zebrafish by lithium-doped hydroxyapatite nanowires LIU Zhen, GUO Wei-ming, LIU Lu, CHEN Rong-jing, FANG Bing Shanghai Journal of Stomatology    2023, 32 (5): 455-461.   DOI: 10.19439/j.sjos.2023.05.002 Abstract （171）      PDF （2023KB）（156）       Knowledge map    PURPOSE: To observe the regulatory effect of lithium-doped hydroxyapatite nanowires on bone metabolism in osteoporotic zebrafish induced by dexamethasone. METHODS: Pure hydroxyapatite nanowires(nHA) and hydroxyapatite nanowires doped with 10% lithium ions (Li-nHA) were prepared by using hydrothermal method, and then material characterization was performed. The juvenile zebrafish cultured for 3 days(3dpf) were selected and co-cultured with nHA and Li-nHA extracts up to 7dpf. A negative(0.1% DMSO) control group was set up and transgenic zebrafish Tg(ola.sp7:nlsGFP) was used to select the best concentration for promoting bone formation. The osteoporotic zebrafish were induced by dexamethasone and incubated with nHA and Li-nHA extracts. The wild-type zebrafish was stained with alizarin red and the osteogenic differentiation was observed in transgenic zebrafish. Real-time quantitative PCR was adopted to detect osteogenic maker genes, such as zinc finger transcription factor (SP7), alkaline phosphatase (ALP), osteoprotegerin (OPG), Runt related transcription factor 2(Runx2) and osteocalcin (OCN). Statistical analysis was performed with GraphPad Prism 9.3 software. RESULTS: nHA and Li-nHA promoted bone formation and up-regulated expression levels of ALP, OCN, Runx2, SP7 and OPG of osteoporotic zebrafish. Compared with nHA, Li-nHA significantly increased the mineralization specific staining area and cumulative optical density of zebrafish bone, and the expression of osteogenic maker genes was also significantly increased. CONCLUSIONS: Doping lithium ions in nano hydroxyapatite can enhance its osteoinductive properties, and Li-nHA can effectively improve bone formation of osteoporotic zebrafish. Reference | Related Articles | Metrics