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Clinical efficacy of the combination of miniscrew with clear aligner in controlling the roller coaster effect MA Xiao-qing, XIANG Fei, FAN Ming-yue, SONG Yi, WANG Xiao-hui, ZHANG Ling, QIAN Wen-hao Shanghai Journal of Stomatology    2022, 31 (2): 193-197.   DOI: 10.19439/j.sjos.2022.02.015 Abstract （996）      PDF （1173KB）（1478）       Knowledge map    PURPOSE: To investigate the clinical efficacy of miniscrew in controlling the roller coaster effect in long-distance space closure with clear aligner. METHODS: Sixteen adult patients with bimaxillary protrusion were selected. All cases were treated with clear aligner and their four first premolars were extracted. G6 was designed in all cases. In the experimental group, four miniscrews were inserted at the beginning of treatment to control the teeth in three dimensions; while in the control group, the miniscrews were not applied until obvious roller coaster effect appeared. Paired t test was used to analyze the data with Graphpad Prism 6.0 software package. RESULTS: All the extraction space was closed successfully and the lateral profile was improved significantly. The torque of the upper incisor was under well control and no roller coaster effect happened in the experimental group; while in the control group, the lingual inclination of the upper incisors and open bite of the posterior teeth developed(P<0.05). After the miniscrews intrusion of the upper anterior teeth, the occlusion relationship improved significantly. CONCLUSIONS: Miniscrews effectively prevents and eliminates the roller coaster effect in the process of long-distance space closure with clear aligner. Reference | Related Articles | Metrics

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Experts consensus on diagnostic and therapeutic strategies for malocclusions at early developing stage FANG Bing, JIN Zuo-lin, BAI Yu-xing, WANG Lin, ZHAO Zhi-he, LI Wei-ran, BAI Ding, HE Hong, LIU Yue-hua, HU Min, SONG Jin-lin, CHEN Li-li, CAO Yang, WU Ting-xi, LI Song, LI Huang, SHI Jie-jun, CHU Feng-ting Shanghai Journal of Stomatology    2021, 30 (5): 449-455.   DOI: 10.19439/j.sjos.2021.05.001 Abstract （1735）      PDF （861KB）（3151）       Knowledge map    Early orthodontic intervention remains a controversial notion in current dental care regime. Whilst early orthodontic treatment for children is the province for orthodontic specialists, a growing number of general practitioners, who may not possess sufficient specialty knowledge, are also involved, raising the concerns about the propriety and quality of their treatment modalities. However, orofacial development of children and adolescents is in a very complicated environment. Comprehensive theoretical knowledge and a great wealth of practical experience are required to ensure the final treatment effect. The possible complications should be kept under control and fully informed to patients and their parents. In order to unify and standardize early orthodontic treatment protocol and help to promote healthy and orderly development of early orthodontic treatment, this consensus summarized the practical experience of orthodontic experts from many famous colleges and affiliated hospitals for clinical reference. Reference | Related Articles | Metrics

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Application of two different comfort techniques in extraction of impacted teeth among 60 patients with hypertension SHAO Yun, CHENG Qing-tao, HE Huan, LI Jing, ZHU Wei-yan, LIU Jia-liang Shanghai Journal of Stomatology    2022, 31 (1): 109-112.   DOI: 10.19439/j.sjos.2022.01.023 Abstract （470）      PDF （574KB）（848）       Knowledge map    PURPOSE: To compare the effect of two comfort techniques of nitrous oxide/oxygen inhalation and dexmedetomidine combined with flurbiprofen axetil sedation and analgesia in impacted teeth extraction among patients with hypertension. METHODS: According to the inclusion and exclusion criteria, sixty patients undergoing impacted teeth extraction with hypertension were randomly divided into 2 groups: X group(nitrous oxide/oxygen inhalation, n=30) and Y group(dexmedetomidine combined with flurbiprofen axetil assisted sedation and analgesia, n=30). The following parameters at T0(preoperative), T1(local anesthesia), T2 (surgical operation), and T3 (5 minutes after surgery) were recorded: mean arterial pressure(MAP), heart rate (HR), Ramsay sedation score, VAS pain score. SPSS 20.0 software package was used for statistical analysis. RESULTS: The parameters at each time point between the two groups were not significantly different (P>0.05). Mood and hemodynamics at T1, T2 and T3 in each group were more stable compared with those at T0(P<0.05). CONCLUSIONS: The two comfort techniques can stabilize the patients' mood, keep the patients' hemodynamics stable, and are safe and effective in extraction of impacted teeth among patients with hypertension. Reference | Related Articles | Metrics

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Shanghai Journal of Stomatology    2006, 15 (1): 28-30.   Abstract （600）      PDF （559KB）（772）       Knowledge map    Related Articles | Metrics

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Evaluation of the effect of Lava Ultimate veneer combined with cast porcelain inlays in the treatment of mild-moderate dentition wear in the elderly patients Zhou Jianying, Wang Jing, Pang Shuting, Zhang Yansheng, Ma Huajing, Feng Yanfen, Zhao Shijun, Hu Kunpeng Shanghai Journal of Stomatology    2025, 34 (2): 208-212.   DOI: 10.19439/j.sjos.2025.02.017 Abstract （246）      PDF （619KB）（440）       Knowledge map    PURPOSE: To explore the effect of Lava Ultimate veneer combined with cast porcelain inlays in the treatment of mild-moderate dentition wear in the elderly. METHODS: A total of 60 elderly patients with mild-moderate dentition wear due to physical factors who underwent treatment from July 2019 to July 2023 were selected and randomly divided into control group(n=30) and experimental group(n=30). The control group received traditional all-ceramic crown restoration treatment, while the experimental group received Lava Ultimate veneers for anterior teeth and porcelain inlays for posterior teeth. The chewing efficiency, prosthesis effectiveness, periodontal index [gingival index(GI), plaque index (PI)], clinical efficacy, aesthetic satisfaction and comfort were compared between the two groups before and after treatment.SPSS 19.0 software package was used for statistical analysis of the data. RESULTS: Before treatment, there was no significant difference in chewing efficiency and absorbance between the two groups(P>0.05). After treatment, masticatory efficiency and absorbance of the experimental group were significantly higher than those of the control group(P<0.05). One week and 6 months after treatment, there was no significant difference in prosthesis margin fit, wear degree, retention, gingival condition, color, shape, secondary caries, restoration fracture, GI and PI between the two groups(P>0.05). At 12 months after treatment,the prosthesis margin fit, retention, gingival condition, GI and PI of the experimental group were significantly better than those of the control group(P<0.05). The total effective rate, satisfaction and comfort of the experimental group were significantly higher than those of the control group(P<0.05). CONCLUSIONS: After treatment with Lava Ultimate veneers for anterior teeth and porcelain inlays for posterior teeth, the masticatory efficiency of the elderly patients with mild-moderate dentition wear is improved, and the prosthesis effect, periodontal index, satisfaction and comfort are significantly improved. Reference | Related Articles | Metrics

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A comparative study on the effects of three types of toothbrushes on the formation of abfraction lesions in teeth Xu Xiaoming, Tian Yingju, Gao Lin, Wang Junyan, Xie Xinyi Shanghai Journal of Stomatology    2025, 34 (4): 422-426.   DOI: 10.19439/j.sjos.2025.04.014 Abstract （230）      PDF （1073KB）（317）       Knowledge map    PURPOSE: To compare the differences in formation of abfraction lesions using three different types of toothbrushes, i.e. mechanical electric toothbrush, sonic electric toothbrush, and ordinary manual toothbrush. METHODS: Seventy-one subjects were recruited publicly and randomly divided into three groups with 24 subjects in the mechanical electric toothbrush group (experimental group 1), another 24 subjects in the sonic electric toothbrush group (experimental group 2), and 23 subjects in the ordinary manual toothbrush group (control group). The observation period was 12-15 months. During the beginning and the end of the experiment, oral digital impressions were obtained by using 3Shape TRIOS 3 intraoral scanning device (Denmark). The two 3D Object (.stl) files obtained were compared and overlapped by using EXO CAD software,and the numerical values of the cervical changes of all canines, first premolars, and second premolars on the labial and buccal sides of the gingival margin after comparison and overlap were measured using 3Shape 3D ViewerTM software. RESULTS: The cervical defect values of the mechanical electric toothbrush group were significantly higher than those of the sonic electric toothbrush group and the ordinary manual toothbrush group (P<0.001), and the cervical defect values of the sonic electric toothbrush group were significantly higher than those of the ordinary manual toothbrush group (P<0.001). There was no significant difference in gender within each group between the mechanical electric toothbrush group and the sonic electric toothbrush group (P>0.05), while the cervical defect values of the male subjects in the ordinary manual toothbrush group were significantly higher than those of the female subjects (P<0.001). The cervical defect value of the mechanical electric toothbrush group was significantly higher than that of the conventional manual toothbrush group at 14, 25, 35, 43, 44 and 45 tooth positions among the three groups (P<0.05). Meanwhile, the cervical defect value of the mechanical electric toothbrush group was significantly higher than that of the sonic electric toothbrush group at 44 tooth position (P<0.05). There was no significant difference in the defect value among all groups (P>0.05). CONCLUSIONS: Compared with ordinary manual toothbrushes, mechanical electric toothbrushes and sonic electric toothbrushes are more effective in promoting the formation of abfraction lesions. The teeth in the lower right mandible are more susceptible to abfraction lesions caused by mechanical electric toothbrushes. Reference | Related Articles | Metrics

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Research progress and current situation of oral surgery robots based on published papers and patents analysis ZHANG Zhi-fan, SHU Zhi-qun, XU Feng, SHEN Hui-qing Shanghai Journal of Stomatology    2025, 34 (1): 106-112.   DOI: 10.19439/j.sjos.2025.01.019 Abstract （446）      PDF （1440KB）（1334）       Knowledge map    PURPOSE: To analyze the research progress and technological development of oral surgery robots, reveal the future development trend of oral surgery robots and put forward policy suggestions. METHODS: Focusing on the topic of oral surgery robots, literature review and patent data retrieval methods were used to analyze data from 2011 to 2023 from the perspectives of quantity, source, and technical field. RESULTS: The number of academic papers and patent applications in the field of oral surgery robots had explosive growth since 2015. The literature mainly focused on topics such as oral implantation and digitalization, with Chinese scholars publishing the most papers. The patents focused on tools such as models and instruments dedicated to oral surgery, as well as technical fields such as implant visual navigation and positioning systems. CONCLUSIONS: Oral surgery robots should be developed towards miniaturization and specialization in the future, and domestic breakthroughs in core technologies such as special instruments need to be accelerated. Reference | Related Articles | Metrics

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Evaluation of biologically oriented preparation technique and conventional dental preparation in total crown restoration Guo Ling, Xu Longfen Shanghai Journal of Stomatology    2025, 34 (4): 398-402.   DOI: 10.19439/j.sjos.2025.04.010 Abstract （295）      PDF （660KB）（389）       Knowledge map    PURPOSE: To evaluate the value of biologically oriented preparation technique and conventional dental preparation in total crown restoration. METHODS: A total of 93 patients who underwent total crown restoration from January 2020 to November 2022 were selected, and were divided into experimental group and control group according to different dental preparation techniques. The control group was treated with conventional dental preparation (46 cases, 74 teeth), while the experimental group was treated with BOPT (47 cases, 75 teeth). Periodontal health indicators [plaque index (PI), gingival index (gingival index), probing depth (PD) and gingival thickness (GT)] were evaluated at 3 months, 6 months, 1 year and 2 years after treatment. The incidence of complications and patients' satisfaction were compared. RESULTS: In the control group, PI increased with time, reaching the highest value (2.20±0.57) at 1 year and decreasing to (1.70±0.66) at 2 years. PI of the experimental group reached the highest value (2.61±0.52) at the 3rd month, decreased at the 6th month, decreased to close to before treatment at the 1st year, and was significantly lower than before treatment at the 2nd year(P<0.001). The GI of the two groups decreased with the extension of time, and the GI of the experimental group at 6 months, 1 year and 2 years after treatment was significantly lower than that of the control group (P<0.001). The PD of the two groups decreased with the extension of time, and the PD of the control group increased at 6 months after treatment, and the PD of the experimental group was significantly lower than that of the control group at 6 months, 1 year and 2 years after treatment (P<0.001). Buccal GT of the two groups decreased with the extension of time, and the GT of the experimental group at 6 months, 1 year and 2 years after treatment was significantly lower than that of the control group (P<0.05). The incidence of biological complications in the experimental group (2.67%) was significantly lower than that in the control group (10.81%)(P<0.05). There was no significant difference in the incidence of mechanical complications between the two groups (P>0.05). The total complication rate of the experimental group (4.00%) was significantly lower than that of the control group (14.86%)(P<0.05). The scores and total scores of satisfaction of the experimental group were significantly higher than those of the control group (P<0.05). CONCLUSIONS: BOPT for total crown repair can reduce PI, GI and PD, increase GT, reduce complications and improve patients' satisfaction. Reference | Related Articles | Metrics

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Metabolomics study on the difference of tongue coating metabolites between patients with intra-oral halitosis and healthy individual LIMAN Aviella-nathania, ZHU Hong-lai, YANG Wen-jie, YE Wei Shanghai Journal of Stomatology    2023, 32 (5): 525-531.   DOI: 10.19439/j.sjos.2023.05.014 Abstract （469）      PDF （1306KB）（1045）       Knowledge map    PURPOSE: To analyze the difference of metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals by untargeted metabolomics, and to explore significant differences in metabolites of intra-oral halitosis as biomarkers. METHODS: The untargeted metabolomics of tongue coating samples from 12 patients with intra-oral halitosis and 12 healthy individuals were studied by liquid chromatography and mass spectrometry combined with principal component analysis and orthogonal partial least squares discriminant analysis. The value of variable importance in projection >1 and P<0.05 of Student's t test in the orthogonal partial least squares-discriminant analysis model were used as the criteria to screen and determine the differential metabolites. RESULTS: There were differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals, and 11 different metabolites were identified. They were valyl-arginine, glycine-phenylalanine, tryptophyl-proline, deoxyadenosine, 4,5-dihydroniveusin A, N-acetyl-DL-tryptophan, paramethasone acetate, cyclopentanol, [(2-hexylcyclopentylidene) amino]thiourea, L-pipecolic acid and taurine. In the intra-oral halitosis group, the expressions of Glycine-phenylalanine and N-acetyl-DL-tryptophan were significantly up-regulated, while the expressions of taurine were significantly down-regulated. CONCLUSIONS: There are differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals. The differential metabolites with diagnostic value may be used as diagnostic markers of intra-oral halitosis. Reference | Related Articles | Metrics

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Exploration of aesthetic perception changes in female patients after extraction orthodontic treatment by non-orthodontic professionals with higher education in Shanghai Chan Iantong, Mao Yanmin, Lu Yun, Lyu Jinzhao, Li Qiang Shanghai Journal of Stomatology    2025, 34 (4): 369-374.   DOI: 10.19439/j.sjos.2025.04.005 Abstract （166）      PDF （1059KB）（543）       Knowledge map    PURPOSE: To explore the factors influencing aesthetic perception changes among non-orthodontic professionals with higher education in Shanghai regarding the maxillofacial appearance of women before and after orthodontic treatment with extractions. METHODS: A total of 18 non-orthodontic professionals (9 men and 9 women) who had received or were receiving higher education in Shanghai were recruited. Using Q-sort technique, the study compared pre- and post-treatment facial soft tissue changes in 48 adult females who underwent orthodontic treatment with extractions. RESULTS: Non-orthodontic professionals with higher education in Shanghai showed significant sensitivity to changes in the lower third of the face, overall facial contour, mouth corners, and chin of women before and after orthodontic treatment (P<0.05). However, changes in the nasolabial fold and upper and lower lips, which were often the focus of orthodontic professionals,didn't show significant differences in aesthetic perception. Female reviewers were more sensitive than male reviewers to aesthetic changes in the chin and lower third of the face, but there was no significant difference in overall facial evaluation. Reviewers born in the 2000s and 1990s demonstrated higher aesthetic sensitivity, while those born in the 1970s and 1980s showed more stable aesthetic evaluations. CONCLUSIONS: Non-orthodontic professionals with higher education experience in Shanghai are more sensitive to the aesthetic changes in the lower 1/3 of the face, chin and other areas after orthodontic treatment with tooth extraction, and the perceived differences are affected by gender and age, which can provide a reference for the design of endpoint goal schemes in clinical orthodontic treatment. Reference | Related Articles | Metrics

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Shanghai Journal of Stomatology    2012, 21 (3): 344-349.   Abstract （278）      PDF （786KB）（607）       Knowledge map    Related Articles | Metrics

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Effect of dental-derived stem cells on the biological characteristics of cementoblasts Li Wenwen, Ma Wensheng, Li Yinghui, Zhao Yuan, Li Dongna, Zhai Haoyan, Liu Chunyan Shanghai Journal of Stomatology    2025, 34 (4): 354-361.   DOI: 10.19439/j.sjos.2025.04.003 Abstract （169）      PDF （2422KB）（275）       Knowledge map    PURPOSE: To investigate the effect of dental-derived mesenchymal stem cells on the biological characteristics of cementoblasts. METHODS: Periodontal ligament stem cells (PDLSCs) and dental pulp stem cell (DPSCs) were isolated, flow cytometry was used to detect cell surface markers. Cementoblasts OCCM-30 were coculture with PDLSCs and DPSCs for 12 h and 24 h. Cell proliferation was examined using the CCK-8 method. ALP staining was used to evaluate the osteogenic differentiation of cementoblasts in different treatment groups. Wound healing migration assays and Transwell migration assays were performed to compare the migration of cementoblasts. The expression of MMP9 and MMP13 was assessed using enzyme-linked immunosorbent assay(ELISA). RESULTS: The expression rates of CD90 and CD34 of PDLSCs were 99.44% and 0.23%. The expression rates of CD90 and CD34 of DPSCs were 99.93% and 3.47%. Their multipotency were illustrated based on differentiation to abiogenic, osteogenic lineages. Human mesenchymal stem cells promoted the proliferation of cementoblasts(P<0.05). The osteogenic differentiation of cementoblasts was increased after stem cells treatment. The migration of cementoblasts was increased after stem cells treatment (P<0.05), and the expression of MMP9 and MMP13 were enhanced. CONCLUSIONS: Dental-derived stem cells can promote the proliferation, osteogenic differentiation and migration of cementoblasts, playing an active role in cementum restoration and providing a scientific basis for promoting the repair process in root resorption. Reference | Related Articles | Metrics

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A retrospective study on clinical outcomes of implant-supported rehabilitations in patients with alveolar cleft Lan Rong, Wu Yiqun, Huang Wei, Chen Zhenqi, Pan Xiaogang, Wang Feng Shanghai Journal of Stomatology    2025, 34 (3): 326-331.   DOI: 10.19439/j.sjos.2025.03.018 Abstract （227）      PDF （921KB）（694）       Knowledge map    PURPOSE: To retrospectively evaluate the clinical outcomes of implant-supported prosthesis in alveolar cleft patients. METHODS: A total of 14 patients with alveolar cleft underwent implant restoration in Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January 2015 to January 2023 were collected. Among them, 9 were male and 5 were female. The age ranged from 18 to 52 years, with a mean of (26.1±10.6) years. The sequence treatment history of patients with cleft lip and palate and the effect of implant repair were retrospectively analyzed. RESULTS: Bone grafting in the fissured area failed repeatedly in 1 patient and no implant was implanted. A total of 21 implants were implanted in the remaining 13 patients with alveolar cleft, all of which achieved good osseointegration. In 10 cases, one implant was implanted in each fissure area for single crown or single end bridge repair. In 3 cases, 11 implants were implanted in the non-fissure area for fixed bridge repair. Seven patients with single implant were followed up for 6 to 96 months, with an average of (39.4±29.2) months. During their visits, patients underwent various examinations, including pink aesthetic score (PES) averaging 10.16±0.98, white aesthetic score(WES) averaging 8.50±1.23, and probing depth(PD) with a mean of (2.68±0.61) mm. Additionally, gingival index(GI) was recorded with a mean of 1.33±1.10, and marginal bone loss(MBL) was measured with a mean of (0.17±0.35) mm. The width of the mid-labial keratinized mucosa was observed to be (5.14±2.10) mm. CONCLUSIONS: Implant-supported prosthesis in the alveolar cleft area after well-established multidisciplinary sequential treatments have predictable middle-term success rate, satisfactory aesthetic outcomes and stable peri-implant soft and hard tissue conditions. Reference | Related Articles | Metrics

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Effect of anxiety and depression symptoms on periodontitis based on Mendelian randomization study Lei Deng, Zhou Yingbin, Huang Sihui, Li Yufeng Shanghai Journal of Stomatology    2025, 34 (4): 414-421.   DOI: 10.19439/j.sjos.2025.04.013 Abstract （183）      PDF （1171KB）（372）       Knowledge map    PURPOSE: To evaluate the effects of anxiety and depression on the risk of periodontitis using Mendelian randomization (MR) method, and to analyze the prevalence and influencing factors of anxiety and depression symptoms in patients with periodontitis, so as to provide evidence for clinical prevention and treatment. METHODS: The data of 70 patients with periodontitis and 35 healthy controls who were treated in the Fourth Hospital of Changsha from August 2022 to August 2024 were collected.Hamilton Anxiety Rating Scale (HAMA) was used to assess the anxiety of the subjects. Hamilton Depression Rating Scale (HAMD) was used to assess symptoms of depression of the subjects, and potential risk factors were analyzed by logistic regression. Single nucleotide polymorphisms (SNP) were screened from the Genome-Wide Association Study (GWAS) database as instrumental variables. The independent SNP were screened according to the P-value threshold (P=5e-05) and the linkage unbalanced clustering method (window size 10 000 kb, r2 threshold 0.001), and the data were coordinated. Five MR methods, including inverse variance weighted (IVW), were used to analyze the causal relationship between anxiety and depression on acute and chronic periodontitis. RESULTS: In the clinical case-control study, HAMA and HAMD scores of the periodontitis group were significantly higher than those of the control group (HAMA: P=0.006; HAMD: P=0.007). The incidence of anxiety in the periodontitis group was significantly higher than that in the control group (P=0.008), and the incidence of depression was also significantly higher (P=0.006). Logistic regression analysis revealed that anxiety, depression, and age ≥ 60 years were independent risk factors for periodontitis (P<0.05). MR analysis showed that anxiety was significantly positively correlated with acute periodontitis (IVW method, P =0.037), and depression was significantly positively correlated with acute periodontitis (IVW method, P=0.043), but neither was significantly associated with chronic periodontitis (P>0.05). The tests for pleiotropy and heterogeneity indicated that the analysis results were reliable (IVW method and MR Egger method, P>0.05 for both). CONCLUSIONS: There is significant correlation between anxiety and depression symptoms and periodontitis. It is recommended to consider the influence of psychological factors in clinical treatment of periodontitis. Reference | Related Articles | Metrics

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Shanghai Journal of Stomatology    2004, 13 (5): 426-429.   Abstract （276）      PDF （733KB）（904）       Knowledge map    Related Articles | Metrics

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The effects of hUCMSC-sEVs on osteogenic/odontogenic differentiation of hDPSCs and tube formation ability of HUVECs Kang Jingyi, Wei Rixia, Deng Huidan, Li Quanjie, Wu Yu Shanghai Journal of Stomatology    2025, 34 (6): 561-570.   DOI: 10.19439/j.sjos.2025.06.001 Abstract （59）      PDF （2345KB）（88）       Knowledge map    PURPOSE: To investigate the effects of small extracellular vesicles (sEVs) derived from human umbilical cord mesenchymal stem cells(hUCMSCs) on the migration and osteogenic/odontogenic differentiation ability of human dental pulp stem cells(hDPSCs), the migration and tube formation ability of human umbilical vein endothelial cells (HUVECs) and their possible mechanisms. METHODS: hUCMSCs, hDPSCs and HUVECs were cultured and identified, hUCMSC-sEVs and LPSpre-hUCMSC-sEVs were isolated and identified, HUVECs and hDPSCs were assigned to 4 kinds of treatments, including the negative control group(NC), the positive control group(PC), the hUCMSC-sEVs group and the LPSpre-hUCMSC-sEVs group. Cell migration ability was detected by Transwell and wound healing assays. Tube formation capacity of HUVECs was assessed by tube formation experiment. The osteogenic/odontogenic differentiation ability of hDPSCs was evaluated by alizarin red staining and RT-qPCR. High-throughput small RNA sequencing was used to define miRNA profiles in sEVs. RESULTS: hUCMSCs, hDPSCs, HUVECs, hUCMSC-sEVs and LPSpre-hUCMSC-sEVs were successfully isolated and identified. Compared with NC group, both LPSpre-hUCMSC-sEVs group and hUCMSC-sEVs group promoted migration of hDPSCs, migration and tube formation of HUVECs. There was no significant difference between LPSpre-hUCMSC-sEVs group and hUCMSC-sEVs group in promoting migration of hDPSCs (P>0.05). LPSpre-hUCMSC-sEVs group was stronger than hUCMSC-sEVs group in promoting migration and tube formation of HUVECs(P<0.05). The calcium salt deposition in LPSpre-hUCMSC-sEVs group and hUCMSC-sEVs group was higher than that in PC group, and the calcium salt deposition in LPSpre-hUCMSC-sEVs group was higher than that in hUCMSC-sEVs group(P<0.05). The mRNA expression levels of ALP, OSX, OCN and RUNX2 in PC group, hUCMSC-sEVs group and LPSpre-hUCMSC-sEVs group were higher than those in NC group(P<0.05), and hUCMSC-sEVs group and LPSpre-hUCMSC-sEVs group were higher than PC group(P<0.05). In addition, the expression levels of OCN and RUNX2 in LPSpre-hUCMSC-sEVs group were higher than those in hUCMSC-sEVs group(P<0.05), while there was no significant difference in ALP and OSX between LPSpre-hUCMSC-sEVs group and hUCMSC-sEVs group(P>0.05). The expression level of DSPP in PC group and hUCMSC-sEVs group was higher than that in NC group, but the difference was not statistically significant(P>0.05). The expression level of DSPP in LPSpre-hUCMSC-sEVs group was higher than that in PC group (P<0.05). The most highly expressed miRNAs including hsa-miR-21-5p, hsa-let-7a-5p, hsa-miR-100-5p, hsa-miR-26a-5p and hsa-miR-222-3p, and differentially expressed miRNAs including hsa-miR-199a-3p, hsa-miR-122-5p, hsa-miR-1246 and hsa-miR-615-3p were detected, which may be the key factors of LPSpre-hUCMSC-sEVs. CONCLUSIONS: Small extracellular vesicles derived from human umbilical cord mesenchymal stem cells can promote migration and osteogenic/odontogenic differentiation of hDPSCs, as well as migration and angiogenesis of HUVECs, and LPS can enhance these effects, which may be related to miRNAs which are the most abundantly and diffferentially expressed in LPSpre-hUCMSC-sEVs. Reference | Related Articles | Metrics

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Nonradiological methods for implant surgical guide accuracy measurement YE Sheng-jia, BAO Shi-jie, CHEN Qu-yi, XIN Xian-zhen, DONG Jian-hui, WEI Bin Shanghai Journal of Stomatology    2021, 30 (5): 467-471.   DOI: 10.19439/j.sjos.2021.05.004 Abstract （470）      PDF （717KB）（807）       Knowledge map    PURPOSE: To evaluate the accuracy of static computer-navigated implantation with surgical guides, based on a non-radiological method. Traditional measurements with a second cone-beam CT (CBCT) were applied to verify the accuracy. METHODS: A total of thirty template-guided implantations were designed and performed on 15 resin models. Two paralleled bone-level implants were planned in the edentulous space of each model, between which the distance was 4 mm. Postoperative implant positions were detected with both CAD/CAM-based measurements applying an intraoral scanner (3Shape TRIOS) and traditional ways via CBCT. Both methods were conducted with a CAD quality-control, reverse engineering software, Geomagic Studio 2013, comparing the positions with the virtual ones. Statistical analysis was processed with SPSS 23.0 software package. RESULTS: Measurements using CBCT (control group) showed a trend toward greater deviations when the results were directly compared(P<0.05). In the CAD/CAM-based evaluation of the 30 samples, the mean deviation of the insertion axis from the planned implant axis was 1.134°. The mean deviations of the implant shoulders in the horizontal direction and at the implant apices were 0.447 mm and 0.557 mm, respectively. No significant difference was observed when measuring distance deviation with the two assessment ways. CONCLUSIONS: Compared with evaluation based on radiology, CAD/CAM based evaluation system is able to evaluate implant accuracy precisely, effectively reduce radiological exposure of patients, being suitable for clinical evaluation. Reference | Related Articles | Metrics

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Genistein loaded by PRF improved bone healing in obese mice ZHANG Xue-bing, LI Qi Shanghai Journal of Stomatology    2024, 33 (1): 13-21.   DOI: 10.19439/j.sjos.2024.01.003 Abstract （421）      PDF （1721KB）（506）       Knowledge map    PURPOSE: To clarify the effect of genistein(GEN) on osteogenic differentiation and explore the effect of GEN loaded by platelet-rich fibrin (PRF) on the repair process of bone defects in obese mice. METHODS: In in vitro experiments, the effect of GEN(0, 0.1, 1, 10, 50 μmol/L) on the proliferation of mouse embryonic osteoblast precursor cells (MC3T3-E1) was determined by CCK 8. Alkaline phosphatase(ALP) staining and quantitative detection of ALP activity were performed to determine the changes of ALP activity in cells; RNA and protein expression levels of ALP, osteopontin (OPN) and osteocalcin (OCN) were detected by quantitative real-time PCR(qRT-PCR) and Western blot. Alizarin red staining was used to define the effect of GEN on mineralization of MC3T3-E1. To verify the feasibility of the PRF drug loading, the ultrastructure of PRF was subsequently observed under SEM. In in vivo experiments, obese C57 mouse models were established by high-fat diet feeding. On this basis, skull defect models with a diameter of 2.8 mm were established, and the prepared GEN/PRF complexes were placed into the bone defect area. The effects of GEN on skull defect repair in obese mice were evaluated by Micro-CT scanning and hematoxylin-eosin(H-E) staining. Statistical analysis was performed with GraphPad Prism 5.0 software package. RESULTS: CCK 8 results showed that 0.1, 1 μmol/L GEN promoted cell proliferation within 7 days(P<0.05); 10 μmol/L GEN had no significant effect on the process of cell proliferation. From the second day, 50 μmol/L GEN significantly inhibited cell growth and showed cytotoxicity(P<0.05). These two concentrations had similar effects in promoting cellular osteogenic differentiation. SEM results showed that PRF presented a 3-dimensional network structure, providing space for loading drug molecules. In in vivo experiments, the body weight of mice in the high-fat diet (HFD) group was 27.7% greater than that in the normal diet group(P<0.05) and had abnormal glucose tolerance (P<0.05). Micro-CT showed that compared with the normal diet group, the number of bone trabeculae in the femur of obese mice was decreased(P<0.05), the distance between bone trabeculae was widened(P<0.05), and the bone density was decreased (P<0.05). In addition, GEN (0.1, 1.0 μmol/L) loaded by PRF increased bone volume fraction in the skull of obese mice (P<0.05). H-E results showed that GEN/PRF promoted the healing of the bone defects. CONCLUSIONS: GEN promotes osteogenic differentiation of MC3T3-E1, and it can effectively accelerate the healing of cranial bone defects after loading with PRF in obese mice. Reference | Related Articles | Metrics

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Analysis of hub genes, pathways and immune checkpoints of CD8+ T cells in metastatic lymph nodes of head and neck squamous cell carcinoma in C3H/He mice ZHOU Di, HAN Nan-nan, LI Hua-sheng, YAN Ming, RUAN Min Shanghai Journal of Stomatology    2024, 33 (6): 586-593.   DOI: 10.19439/j.sjos.2024.06.004 Abstract （429）      PDF （2332KB）（1025）       Knowledge map    PURPOSE: To explore the expression of hub genes, pathways and inhibitory immune checkpoints of CD8+ T cells in metastatic lymph nodes of head and neck squamous cell carcinoma(HNSCC) in C3H/He mice. METHODS: A popliteal lymph node metastasis model of immunocompetent C3H/He mice was constructed with SCC-7 cell line of HNSCC, and the lymph node metastasis status was determined by immunofluorescence. CD8+ T cells in normal and metastatic lymph nodes were sorted by flow cytometry, and transcriptome sequencing analysis was performed to screen out differentially expressed genes. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed sequentially. Flow cytometry and multiplex immunohistochemical were used to detect the expression of four immune checkpoints of CD8+ T cells in metastatic lymph nodes of tumor-bearing mice. SPSS 26.0 software package was used for statistical analysis. RESULTS: After 4 weeks of foot pad injection of SCC-7 cells, C3H/He mice displayed obvious metastasis in popliteal drainage lymph node. A total of 912 differentially expressed genes were screened out by transcriptome sequencing analysis of CD8+ T cells in normal and metastatic lymph nodes, including three inhibitory immune checkpoint-related genes which were upregulated(Pdcd1, Lag3 and Tigit). Eight tumor-related signaling pathways were screened out by KEGG network analysis, including Toll-like receptor signaling pathway, NF Kappa-B signaling pathway, TNF signaling pathway, MAPK signaling pathway, IL-17 signaling pathway, NOD-like receptor signaling pathway, PD-L1 and PD-1 immune checkpoint pathway in tumors and p53 signaling pathway. Flow cytometry showed high expression level of PD-1 and TIM-3 in CD8+ T cells in mouse metastatic lymph nodes. It was further confirmed by multiplex immunohistochemical that PD-1 was highly expressed in CD8+ T cells in metastatic lymph nodes of patients with HNSCC. CONCLUSIONS: Tumor-related signaling pathway of CD8+ T cells in mouse metastatic lymph nodes is significantly activated. The PD-1 expression level of CD8+ T in metastatic lymph nodes of mice and patients with HNSCC is markedly increased. Immunotherapy targeting CD8+ T cells may become a new strategy for the prevention and cure for lymph node metastasis of HNSCC. Reference | Related Articles | Metrics

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Segmentation and validation of mandibular canal and its bifurcation on cone beam CT based on deep learning Ye Ye, Fang Shuobo, Lu Huitong, Liu Mingqian, Wu Xueying Shanghai Journal of Stomatology    2025, 34 (2): 119-125.   DOI: 10.19439/j.sjos.2025.02.002 Abstract （425）      PDF （1649KB）（781）       Knowledge map    PURPOSE: To train the U-net of convolutional neural network to establish a method for detecting and segmenting the mandibular canal and its bifurcation, and validate its accuracy based on the ground truth labeled by experts. METHODS: A total of 290 CBCT scans were collected from Shanghai Stomatological Hospital from Jan. 2022 to Dec. 2022, which were divided into training set of 200 scans and test set of 90 scans. Model training included two steps. In the first step, bilateral mandibular canals and its bifurcation of 50 CBCT scans were labeled in 3D Slicer image computing platform by investigators. Three dimensional U-net segmentation model were trained initially with data enhancement. A morphological post-processing method was applied to the predicted results. In the second step, pseudo label method was employed to help annotating the mandibular canal and corresponding bifurcations on remaining 150 CBCTs, which would be included in training set after revision. Three dimensional U-net model was trained based on these 200 data. During test phase, totally 90 scans were labeled by two doctors and U-net model respectively. Consistency check was conducted to evaluate the labels between two doctors. Dice similarity coefficient and Hausdorff distance were calculated to evaluate the labels between doctors and the model. The detection rate of bifurcation was calculated. SPSS 20.0 software package was used for data analysis. RESULTS: In 90 CBCT test set, the Kappa value between two dentists' annotations was 0.667. The average Dice and Hausdorff distance between predictions and labels of doctors were (0.739±0.068) and (0.988±1.14) mm. In bifurcation detection, the detection rate was 91.30% on scans with clear bifurcations. CONCLSIONS: The dentification and segmentation U-net model of mandibular canal on dental CBCT can be reliable and practical for its high segmentation precision and predicting speed. Reference | Related Articles | Metrics