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Metabolomics study on the difference of tongue coating metabolites between patients with intra-oral halitosis and healthy individual LIMAN Aviella-nathania, ZHU Hong-lai, YANG Wen-jie, YE Wei Shanghai Journal of Stomatology    2023, 32 (5): 525-531.   DOI: 10.19439/j.sjos.2023.05.014 Abstract （352）      PDF （1306KB）（674）       Knowledge map    PURPOSE: To analyze the difference of metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals by untargeted metabolomics, and to explore significant differences in metabolites of intra-oral halitosis as biomarkers. METHODS: The untargeted metabolomics of tongue coating samples from 12 patients with intra-oral halitosis and 12 healthy individuals were studied by liquid chromatography and mass spectrometry combined with principal component analysis and orthogonal partial least squares discriminant analysis. The value of variable importance in projection >1 and P<0.05 of Student's t test in the orthogonal partial least squares-discriminant analysis model were used as the criteria to screen and determine the differential metabolites. RESULTS: There were differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals, and 11 different metabolites were identified. They were valyl-arginine, glycine-phenylalanine, tryptophyl-proline, deoxyadenosine, 4,5-dihydroniveusin A, N-acetyl-DL-tryptophan, paramethasone acetate, cyclopentanol, [(2-hexylcyclopentylidene) amino]thiourea, L-pipecolic acid and taurine. In the intra-oral halitosis group, the expressions of Glycine-phenylalanine and N-acetyl-DL-tryptophan were significantly up-regulated, while the expressions of taurine were significantly down-regulated. CONCLUSIONS: There are differences in the metabolites of tongue coating between patients with intra-oral halitosis and healthy individuals. The differential metabolites with diagnostic value may be used as diagnostic markers of intra-oral halitosis. Reference | Related Articles | Metrics

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A reflectional insight into orthodontic theoretical renovation and technological innovation SHEN Gang Shanghai Journal of Stomatology    2023, 32 (2): 113-119.   DOI: 10.19439/j.sjos.2023.02.001 Abstract （600）      PDF （867KB）（572）       Knowledge map    Orthodontics, among other specialties in dentistry, remains most dynamic and vigorous in upgrading and reforming its fundamental doctrines and clinical technologies. Orthodontic specialty in China has played a leading role in recent years in reshaping fundamental theories and in creating cutting-edge therapeutic modalities. The newly developed diagnostic classification system, a complementary addition to that of Angle's, not only defines the natures, but also identifies the developmental mechanisms of malocclusions. Orthopedic therapy aiming to relocate the mandible before correcting the dentition is emerging as an indispensable treatment mode to malocclusions concurring with mandibular deviation. Whilst correlations exist between malocclusion and TMD susceptibility and incidence, the specifically designed orthopedic and orthodontic approaches have proven effective in managing cases inflicted by TMD conditions. Innovative efforts that create the GS products, have redefined the clear appliances much more beyond merely clear aligners, thus further pushing the boundaries of both the indications and clinical use for clear appliances. Reference | Related Articles | Metrics

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Analysis of hub genes, pathways and immune checkpoints of CD8+ T cells in metastatic lymph nodes of head and neck squamous cell carcinoma in C3H/He mice ZHOU Di, HAN Nan-nan, LI Hua-sheng, YAN Ming, RUAN Min Shanghai Journal of Stomatology    2024, 33 (6): 586-593.   DOI: 10.19439/j.sjos.2024.06.004 Abstract （252）      PDF （2332KB）（560）       Knowledge map    PURPOSE: To explore the expression of hub genes, pathways and inhibitory immune checkpoints of CD8+ T cells in metastatic lymph nodes of head and neck squamous cell carcinoma(HNSCC) in C3H/He mice. METHODS: A popliteal lymph node metastasis model of immunocompetent C3H/He mice was constructed with SCC-7 cell line of HNSCC, and the lymph node metastasis status was determined by immunofluorescence. CD8+ T cells in normal and metastatic lymph nodes were sorted by flow cytometry, and transcriptome sequencing analysis was performed to screen out differentially expressed genes. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed sequentially. Flow cytometry and multiplex immunohistochemical were used to detect the expression of four immune checkpoints of CD8+ T cells in metastatic lymph nodes of tumor-bearing mice. SPSS 26.0 software package was used for statistical analysis. RESULTS: After 4 weeks of foot pad injection of SCC-7 cells, C3H/He mice displayed obvious metastasis in popliteal drainage lymph node. A total of 912 differentially expressed genes were screened out by transcriptome sequencing analysis of CD8+ T cells in normal and metastatic lymph nodes, including three inhibitory immune checkpoint-related genes which were upregulated(Pdcd1, Lag3 and Tigit). Eight tumor-related signaling pathways were screened out by KEGG network analysis, including Toll-like receptor signaling pathway, NF Kappa-B signaling pathway, TNF signaling pathway, MAPK signaling pathway, IL-17 signaling pathway, NOD-like receptor signaling pathway, PD-L1 and PD-1 immune checkpoint pathway in tumors and p53 signaling pathway. Flow cytometry showed high expression level of PD-1 and TIM-3 in CD8+ T cells in mouse metastatic lymph nodes. It was further confirmed by multiplex immunohistochemical that PD-1 was highly expressed in CD8+ T cells in metastatic lymph nodes of patients with HNSCC. CONCLUSIONS: Tumor-related signaling pathway of CD8+ T cells in mouse metastatic lymph nodes is significantly activated. The PD-1 expression level of CD8+ T in metastatic lymph nodes of mice and patients with HNSCC is markedly increased. Immunotherapy targeting CD8+ T cells may become a new strategy for the prevention and cure for lymph node metastasis of HNSCC. Reference | Related Articles | Metrics

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Application of sliding vermilion flap in angulus oris defect after buccal mucosa cancer ablation Wu Kun, Wu Hanjiang Shanghai Journal of Stomatology    2025, 34 (2): 157-161.   DOI: 10.19439/j.sjos.2025.02.008 Abstract （114）      PDF （1469KB）（533）       Knowledge map    PURPOSE: To evaluate the effect of sliding vermilion flap in angulus oris defect after buccal mucosal cancer ablation. METHODS: A total of 33 patients with buccal mucosal cancer who underwent buccal mucosal cancer radical surgery and resection of angulus oris, and reconstructed with free anterolateral thigh flap transfer and sliding vermilion flap were collected from January 2015 to April 2021 in Department of Oral and Maxillofacial Surgery at Second Xiangya Hospital. Thirty-three patients with buccal mucosal cancer admitted to Department of Oral and Maxillofacial Surgery, Second Xiangya Hospital of Central South University from January 2015 to April 2021 were selected. All patients underwent combined buccal-mandibulectomy and neck radical operation and resection of oral angle, and underwent anterolateral femoral free flap and lip red elastic flap reconstruction. The angulus oris outcome, aesthetics outcome, masticatory efficacy, swallowing and speech function were assessed by FACT-HN (The Functional Assessment of Cancer Therapy-Head and Neck). The mouth opening was measured and recorded. SPSS 19.0 software package was used for statistical analysis of the data. RESULTS: The success rate of free flap transplantation was 100% in 33 patients. Although the aesthetics outcome was decreased at postoperative 1 year compared with that of preoperative duration (P<0.001), there was no significant difference of angulus oris outcome at postoperative 1 year compared with that of preoperative duration (P>0.05). Moreover, the masticatory function was significantly improved at postoperative 1 year compared with that of preoperative duration(P=0.004). However, there was no significant difference of speech and swallowing function at postoperative 1 year compared with that of preoperative duration (P>0.05). The mouth opening was significantly improved at postoperative 1 year compared with that of preoperative duration(P<0.05), and the satisfaction of mouth opening in these patients was 75.7%. CONCLUSIONS: The use of sliding vermilion flap can ideally repair the angulus oris, and offer a novel method to reconstruct defect of angulus oris. Reference | Related Articles | Metrics

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Segmentation, accuracy validation of mandibular molar, pulp cavity on cone-beam CT images by U-net neural network LIN Xiang, FU Yu-jie, REN Gen-qiang, WEN Jia-huan, CHEN Yu-fei, ZHANG Qi Shanghai Journal of Stomatology    2022, 31 (5): 454-459.   DOI: 10.19439/j.sjos.2022.05.002 Abstract （597）      PDF （912KB）（531）       Knowledge map    PURPOSE: To realize the automatic segmentation of mandibular molar and pulp cavity on cone-beam CT (CBCT) images by U-net convolutional neural network, and to use the 3D models reconstructed by Micro-CT data as the ground truth to validate its accuracy. METHODS: Twenty groups of small field of view(FOV) CBCT data containing complete mandibular molars were collected from the Department of Radiology, Affiliated Stomatology Hospital of Tongji University. After preprocessing, an endodontic specialist labeled teeth and pulp cavities by MITK Workbench software. These data were used as the training set for training U-net neural network. In addition, five mandibular molars and corresponding small FOV CBCT data were collected. These five CBCT were processed in the same way and used as the testing set. Then, teeth and pulp cavities on CBCT images of the testing set were segmented and reconstructed by U-net neural network and the same specialist. The isolated teeth were scanned by a Micro-CT machine after preprocessing and the results were reconstructed to 3D models, which were used as the ground truth. Then the 3D models reconstructed by the specialist’s labeling, U-net network segmentation results, and the ground truth in the testing set were compared. Dice similarity coefficient(DSC), average symmetric surface distance (ASSD), Hausdorff distance (HD), and morphological analysis were used to evaluate the results. SPSS 20.0 software package was used for statistical analysis. RESULTS: Compared with the ground truth, the segmentation accuracy of the U-net neural network measured by DSC, ASSD, and AHD was (95.30±1.01)%, (0.11±0.02) mm, and (1.05±0.31) mm in teeth and (81.21±2.27)%, (0.15±0.05) mm, and (3.29±1.85) mm in the pulp cavity, respectively. Morphological analysis results showed that the U-net network segmentation results were similar to the ground truth in tooth and pulp chamber. As for the segmentation results of root canals, only thick root canals could be segmented rather than the thin root canals, such as the canals in the apical third and lateral root canals. CONCLUSIONS: Under the experimental conditions, the U-net neural network trained by the specialist’s labeling realized the automatic and accurate segmentation of mandibular molar and their pulp chamber on CBCT images. For the segmentation of root canals, the results need to be further improved. Reference | Related Articles | Metrics

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Evaluation of red and white aesthetic effects of porcelain veneers in the restoration of developmental anterior dental gaps DENG Xiao-lin, YUAN Shuang, ZHU Dan-ting, SHI Le Shanghai Journal of Stomatology    2023, 32 (3): 276-279.   DOI: 10.19439/j.sjos.2023.03.011 Abstract （409）      PDF （873KB）（523）       Knowledge map    PURPOSE: To evaluate the red and white aesthetic effect of porcelain veneer in the restoration of developmental anterior interdental spaces. METHODS: A total of 152 anterior teeth in 64 patients with developmental anterior dental gaps were restored using porcelain veneers, the aesthetic effects before and after restoration were evaluated by pink aesthetic index (PES) and white aesthetic index(WES), the aesthetic effect of gingival papilla filling and reconstruction was evaluated by interdental gingival papilla index (PIS), and visual analogue score (VAS) was used to compare the satisfaction of patients before and after restoration. SPSS 203.0 software package was used for statistical analysis. RESULTS: The overall mean scores of PES before and after restoration of 152 developmental anterior interdental teeth were 9.63±2.23 and 13.64±0.88, respectively. The average scores of WES before and after restoration were 6.85±1.87 and 9.81±0.58, respectively. There were significant differences of PES and WES scores before and after restoration(P<0.01). According to the requirements of "near" perfect restoration(PES≥13 points, WES≥9 points), the red and white aesthetic effect after restoration was near the standard. The scores of PIS before and after restoration were 1.86±0.67 and 2.97±0.18, the interdental gingival papilla was completely filled with space, and the shape was ideal, there were significant statistical differences before and after restoration(P<0.01). Patients were more satisfied with the smile curve and morphology of the restored anterior teeth than other parameters. CONCLUSIONS: The aesthetic effect of using porcelain veneers to repair developmental anterior interdental gaps is ideal, among which the filling of the papillae between the teeth can meet the expectations of patients, and the aesthetic effect evaluation of PIS and PES/WES applied to porcelain veneers to repair developmental anterior interdental spaces has practical guiding significance in clinical practice. Reference | Related Articles | Metrics

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Short-term efficacy of three root canal filling methods in endodontic treatment of chronic periapical inflammation GUO Jing-jing, TANG Yi-qun, HE Hong, XU Zhen Shanghai Journal of Stomatology    2022, 31 (5): 544-549.   DOI: 10.19439/j.sjos.2022.05.018 Abstract （617）      PDF （684KB）（504）       Knowledge map    PURPOSE: To provide a reference basis for clinical selection of a reasonable and effective root canal treatment by comparing the short-term clinical efficacy of three root canal filling methods in the treatment of chronic periapical inflammation. METHODS: One hundred and twenty patients with chronic periapical inflammation who received root canal filling between October 2019 and October 2020 were randomly divided into 3 groups, with 40 patients in each group. All patients received root canal filling, group A was filled with iRoot SP paste by matched-taper single cone obturation technique, group B was filled with AH-plus paste by warm vertical condensation, and group C filled with AH-plus paste by cold lateral condensation. Root canal filling time in the three groups was calculated, pain score 24 h after treatment was determined using visual analogue scale/score(VAS), and periapical index(PAI) was used to evaluate radiographs. SPSS 22.0 statistical software was applied for data analysis. RESULTS： Root canal filling times in the three groups were (75.50±7.44) s in group A, (85.38±3.46) s in group B and (102.33±3.32) s in group C, the differences were statistically significant (P<0.05); the incidence of postoperative pain in the three groups was 25% in group A, 25% in group B and 32.5% in group C, the difference was not statistically significant(P>0.05). There was no significant difference between group A and group B, but significant difference existed between group A, group B and group C. The results of comparison of PAI scores showed a time effect was F=498.93, P<0.001, suggesting significant difference in total PAI scores at different time points; a group effect was F=0.91, P=0.406, suggesting no significant difference in total PAI scores of the 3 groups; an interaction effect of time and group was F=0.44, P=0.777, suggesting that there was no significant difference in total PAI scores of the 3 groups at the time points. The total effective rate at 3 and 12 months was 97.5%, 97.3% in group A, 97.5%, 97.2% in group B and 90%, 91.9% in group C, the difference was not statistically significant(P>0.05). CONCLUSIONS: Compared with the other 2 root canal filling methods, the iROOT SP paste matched-taper single cone obturation technique is clinically effective in terms of time saving and increasing the comfort of the patients' visit. Reference | Related Articles | Metrics

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Application of 3D printing modified tooth-supported cyst plug in decompression of mandibular cystic lesions KOU Peng, LI Ying-mei, LI Zhi-ping, MENG Jian Shanghai Journal of Stomatology    2023, 32 (2): 203-208.   DOI: 10.19439/j.sjos.2023.02.017 Abstract （495）      PDF （1849KB）（503）       Knowledge map    PURPOSE: To evaluate the application value of 3D printing modified dental support cyst plug in fenestration of large jaw cystic lesions. METHODS: Forty patients with mandibular cystic disease in Xuzhou Central Hospital from October 2019 to April 2021 were selected. They were randomly divided into experimental group(3D printing group) and control group (traditional plug group), with 20 cases in each group. All enrolled patients underwent preoperative digital modeling of cystic lesions of the jaw, obtained the cystic cavity volume data of preoperative lesions, designed the windowing site according to the plan and performed decompression for jaw cysts. Three days after surgery, the patient's postoperative CBCT and Oral-scan data in the experimental group was obtained, and a digitally modified tooth-supported cyst plug with porous column channel was designed, and titanium alloy material for 3D printing was selected. In the control group, the plug was manually molded by experienced physicians. The visual analogue scale(VAS) score of pain, retention, mechanical properties of the plug and its effect on the adjacent teeth were compared between the two groups during the process of model preparation, and the changes of the cyst volume 1, 3 and 6 months after operation were compared between the two groups. SPSS 25.0 software package was used for data analysis. RESULTS: Compared with the control group, the patients in the experimental group who made titanium alloy as printing material by digital impression complained more comfortable, and the mechanical strength and stability of the cyst plug were better than those in the control group(P< 0.05). There was no significant difference in retention between the two groups(P>0.05). The reduction rate of cyst volume in the experimental group was significantly higher than that in the traditional plug group 3 and 6 months after operation(P<0.05). CONCLUSIONS: The modified tooth-supported titanium alloy cyst plug with digital 3D printing has good mechanical properties and stability. It has little damage to the abutment and no lateral force, and has the advantages of precision, individualization and comfort. The improved irrigation and injection channel can fully flush the cavity and speed up the narrowing of the cyst and reduce the waiting time before the second operation, which is worth promoting in clinical practice. Reference | Related Articles | Metrics

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Repair of mandibular defects with hydrogel loaded with nano-hydroxyapatite in rats XU Lai-jun, YUAN He, YE Qing, LI Ji-yao Shanghai Journal of Stomatology    2022, 31 (5): 449-453.   DOI: 10.19439/j.sjos.2022.05.001 Abstract （458）      PDF （2961KB）（471）       Knowledge map    PURPOSE: To observe the effect of gellan gum loaded with nano-hydroxyapatite (GG/nHA) on repairing mandibular defect in rats. METHODS: Critical bone defects with 5 mm in diameter on the mandible of 16 SD rats were created and randomly divided into two groups. The bone defects in the experimental group were injected with GG/nHA and the control group were filled with absorbable gelatin sponge. The rats were sacrificed at 4 and 8 weeks after operation. The bone tissue healing was evaluated by Micro-CT. Bone tissue repairing effect was evaluated by hematoxylin-eosin (H-E) staining and Masson staining. GraphPad Prism 8.0 software package was used for statistical analysis. RESULTS: The prepared GG/nHA had a good injectability and could be delivered to the bone defect area with a syringe. Four and 8 weeks after operation, the newly formed bone and bone volume fraction (BV/TV) in the experimental group were higher than those in the control group, and the difference was statistically significant (P<0.05). Larger number of new bone were observed in the experimental group than the control group by H-E staining and Masson staining. CONCLUSIONS: GG/nHA can be injected into the mandibular defect area to promote its healing, and it is expected to be used as a novel bio-material for minimally invasive repair of oral and maxillofacial bone defects. Reference | Related Articles | Metrics

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Enhanced mechanical properties, cytocompatibility of GelMA hydrogels by incorporating graphene oxide BAO Jing, QIN Wei, ZHU Qing, QIAN Wen-hao Shanghai Journal of Stomatology    2022, 31 (5): 491-496.   DOI: 10.19439/j.sjos.2022.05.008 Abstract （395）      PDF （1321KB）（468）       Knowledge map    PURPOSE: To construct graphene oxide/methacrylated gelatin(GO/GelMA) hydrogel and to investigate its mechanical property and biocompatibility. METHODS: 50, 100, 200 μg/mL GO was added to GelMA and mixed thoroughly. The micromorphology of hydrogels was observed under scanning electron microscope(SEM). Compression text was used to assess lastic modulus, CCK-8 and live/dead cell staining was conducted to assess the viability of rat bone marrow-derived mesenchymal stem cells (rBMSCs). The data were analyzed using SPSS 23.0 software package. RESULTS: SEM showed a highly porous structure of the hydrogel. After adding GO, the elastic modulous increased (100, 200 μg/mL), swelling ratio increased(100 μg/mL) and expansion ratio decreased(200 μg/mL). The viability increased in 50 μg/mL GO and decreased in 100 and 200 μg/mL GO. CONCLUSIONS: Proper concentration of GO can increase the mechanical property and biocompatibility. 50 μg/mL GO/GelMA was suggested in bone tissue engineering given priority to biocompatibility. Reference | Related Articles | Metrics

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Feasibility of precise digital techniques combined with bone puncture techniques to guide the extent of jaw resection LIU Chang, WU Qiong, CHEN Shao-hua, XU Tao, LIU Liang, ZHANG Kai Shanghai Journal of Stomatology    2023, 32 (5): 519-524.   DOI: 10.19439/j.sjos.2023.05.013 Abstract （310）      PDF （1534KB）（461）       Knowledge map    PURPOSE: To evaluate the feasibility of Mimics software combined with 3D printing and bone biopsy to guide the resection scope of mandibular malignant tumors. METHODS: Fifteen patients admitted to the Department of Oral and Maxillofacial Surgery of the First Affiliated Hospital of Bengbu Medical College from January 2019 to April 2021 for oral malignancies and requiring mandibular osteotomy were selected. Preoperatively, CT data of the mandibles were taken to design and print the puncture guide using Mimics software and 3D printing technology, and the length of the osteotomy and the number of teeth lost were also measured. The resected mandible was then combined with the puncture guide and a percutaneous puncture needle to perform bone puncture at the appropriate site and sent for pathology. The mandibular gross specimen was decalcified and when decalcified, the mandibular gross was taken from the same loci, thus testing the accuracy of the bone puncture pathology relative to the gross decalcification pathology, comparing the actual osteotomy length with the simulated osteotomy length and comparing the actual number of missing teeth with the simulated number of missing teeth. SPSS 17.0 software package was used for data analysis. RESULTS: Bone puncture pathology using 3D guides possessed consistent accuracy with gross decalcification pathology. Simulated osteotomy length was significantly less than actual osteotomy length(P<0.001). Simulated number of missing teeth was significantly less than actual number of missing teeth (P<0.01). CONCLUSIONS: The use of Mimics software combined with bone puncture biopsy techniques to guide the extent of resection of malignant tumors in the mandible allows for more accurate mandibular osteotomy, thereby preserving more healthy bone and teeth. Reference | Related Articles | Metrics

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Comparison of clear aligners and customized lingual appliance for bimaxillary dentoalveolar protrusion: a retrospective study LI Zhen-xia, YUAN Ling-jun, ZHENG Xiao-wen, LIU Lu, LIU Chao, FANG Bing Shanghai Journal of Stomatology    2023, 32 (5): 480-484.   DOI: 10.19439/j.sjos.2023.05.006 Abstract （314）      PDF （629KB）（459）       Knowledge map    PURPOSE: To compare the treatment effects of clear aligners and customized lingual appliance on treating bimaxillary dentoalveolar protrusion patients with first premolar extractions. METHODS: Fifty-four patients with bimaxillary dentoalveolar protrusion treated in Shanghai Ninth People Hospital were involved in the retrospective study. Twenty-five cases used clear aligners and 29 cases used customized lingual appliance. All of them were treated by extracting 4 first premolars and retracting anterior teeth with strong anchorage. The changes of anterior tooth and soft tissue adduction before and after treatment were compared by lateral cephalometric measurements. SPSS 26.0 software package was used for data analysis. RESULTS: The total course of treatment in the clear aligners group (46.32±7.37 months) was about 10.8 months longer than that in the customized lingual appliance group (35.55±5.90 months) (P<0.05). There was no significant difference in upper incisor retraction, lower incisor inclination and overjet reduction between the two groups(P>0.05). There were significant differences in upper lip retraction, lower lip retraction, upper incisor torque reduction, and overbite reduction between the two groups(P<0.05). Customized lingual appliance group showed a significant improvement of lips retraction and overbite reduction in orthodontic treatment. For the correction of overjet, there was no significant difference between the two groups (P=0.337). The data of U1-OP (the distance between edge of the upper central incisor and the functional occlusal plane) was not in normal distribution, and there was no significant difference between the two groups(P=0.184). CONCLUSIONS: The two techniques can both retract the anterior teeth and lips to improve the profile. However, the customized lingual appliance was more effective in improving the soft tissue profile of patients with bimaxillary dentoalveolar protrusion, with shorter treatment course. Reference | Related Articles | Metrics

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Biological characteristics and osteogenic differentiation of magnesium-doped nanoporous titanium coating ZHAO Shan, ZHANG Lin, LI Sheng-nan, KANG Nan, MENG Jian, LI Xiao-dong Shanghai Journal of Stomatology    2024, 33 (1): 6-12.   DOI: 10.19439/j.sjos.2024.01.002 Abstract （309）      PDF （2088KB）（456）       Knowledge map    PURPOSE: Bioactive magnesium ions were successfully incorporated into the nanoporous titanium base coating by micro-arc oxidation(MAO), and its physical properties and osteogenic effects were explored. METHODS: Non-magnesium-containing and magnesium-containing titanium porous titanium coatings(MAO, MAO-mg) were prepared by changing the composition of MAO electrolyte and controlling the doping of magnesium in porous titanium coatings. The samples were characterized by scanning electron microscope (SEM), roughness, contact angle and energy dispersive X-ray spectrometer (EDS). Mg2+ release ability of magnesium-doped nanoporous titanium coatings was determined by inductively coupled plasma/optical emission spectrometer(ICP-OES). The structure of the cytoskeleton was determined by live/dead double staining, CCK-8 detection of material proliferation-toxicity, and staining of β-actin using FITC-phalloidin. The effects of the coating on osteogenic differentiation in vitro were determined by alizarin red (ARS), alkaline phosphatase (ALP) staining and real-time polymerase chain reaction (qRT-PCR). SPSS 25.0 software package was used for statistical analysis. RESULTS: The MAO electrolyte with magnesium ions did not change the surface characteristics of the porous titanium coating. Each group prepared by MAO had similar microporous structure(P>0.05). There was no significant difference in surface roughness and contact angle between MAO treatment group (MAO, MAO-mg)(P>0.05), but significantly higher than that of Ti group (P<0.05). With the passage of cell culture time, MAO-mg group promoted cell proliferation (P<0.05). MAO-mg group was significantly higher than other groups in ALP and ARS staining. The expression of Runx2 mRNA (P<0.05), ALP(P<0.05) and osteocalcin OCN(P<0.05) in MAO-mg group was significantly higher than that in Ti and MAO groups. CONCLUSIONS: MAO successfully prepared magnesium-containing nanoporous titanium coating, and showed a significant role in promoting osteogenic differentiation. Reference | Related Articles | Metrics

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Curcumin targeting miR-155-5p/TP53INP1axis induced oxidative stress to regulate salivary gland tumor cell proliferation, apoptosis DU Hong-liang, HE Deng-qi, WANG Jia-Li, CHE Yin-fu, YAN Qing-han Shanghai Journal of Stomatology    2022, 31 (5): 483-490.   DOI: 10.19439/j.sjos.2022.05.007 Abstract （353）      PDF （1709KB）（453）       Knowledge map    PURPOSE: To investigate the mechanism of curcumin targeting miR-155-5p/TP53INP1 axis to induce oxidative stress to regulate salivary gland tumor cell proliferation and apoptosis. METHODS: A253 cells were cultured by adding curcumin and transfected with miR-155-5p mimic and/or pcDNA3.1-TP53INP1. Cell proliferation was detected by CCK-8 assay cell apoptosis was detected by flow cytometry, cell migration ability was detected by scratch test. The targeting relationship between miR-155-5p and TP53INP1 was verified by dual luciferase reporter assay. miR-155-5p, TP53INP1 mRNA expression was detected by qRT-PCR. Western blot was performed to detect expression of TP53INP1, Caspase8, Caspase3, Bcl-2, Bax protein; and ELISA was used to determine SOD, Gpx, and MDA content. Statistical analysis was performed using SPSS 22.0 software package. RESULTS: Dual luciferase reporter assay confirmed that TP53INP1 was a downstream target regulatory molecule of miR-155-5p. Compared with DMSO group, cell apoptosis, Caspase8, Caspase3, Bax protein expression and TP53INP1 expression were significantly increased in curcumin group, while Bcl-2 protein expression, miR-155-5p mRNA and number of cell migration were significantly decreased(P<0.05). Compared with curcumin + miR-155-5p mimic group, cell apoptosis, Caspase8, Caspase3, Bax protein expression was significantly increased in curcumin + pcDNA3.1-TP53INP1 group and curcumin + miR-155-5p mimic + pcDNA3.1-TP53INP1 group; Bcl-2 protein expression was significantly increased(P<0.05), SOD, GSH-PX activities and number of cell migration were significantly decreased and MDA content was significantly increased in curcumin+pcDNA3.1-TP53INP1 group (P<0.05). CONCLUSIONS: Curcumin inhibited A253 cell proliferation and promoted A253 cell apoptosis. The mechanism may be related to targeting miR-155-5p/TP53INP1 axis to induce oxidative stress regulation. Reference | Related Articles | Metrics

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Comparative analysis of the effect of autologous dentin particles, implantation of Bio-Oss on guided bone regeneration in the treatment of periodontal bone defects MAO Xin-xia, LIN Xiao-wei, WEI Xuan, LI Qin Shanghai Journal of Stomatology    2022, 31 (5): 530-534.   DOI: 10.19439/j.sjos.2022.05.015 Abstract （508）      PDF （597KB）（450）       Knowledge map    PURPOSE: To analyze the effect of autologous dentin particles and Bio-Oss bone powder implantation on guided bone regeneration in the treatment of periodontal bone defects. METHODS: Eighty-two patients with periodontal bone defect treated in Zhangye People's Hospital from May 2018 to May 2020 who underwent guided bone regeneration treatment were selected and divided into experimental group and control group according to random number table method, with 41 cases in each group. The control group was implanted with Bio-Oss during the operation, and the experimental group was implanted with autologous dentin particles during operation. Periodontal pocket depth and attachment loss, buccal soft tissue profile, buccal soft tissue thickness, red aesthetic score, success rate, and complications were compared between the two groups of patients. SPSS 22.0 software package was used for statistical analysis. RESULTS: Comparison within the group showed that 1 month and 1 year after operation, the periodontal pocket depth and attachment loss of the two groups were significantly reduced(P<0.05); and 1 year after operation, the periodontal pocket depth and attachment loss of the two groups were significantly lower than 1 month after surgery(P<0.05). Comparison between groups showed that 1 month and 1 year after operation, the periodontal pocket depth and attachment loss of the experimental group were significantly lower than those of the control group (P<0.05). Comparison within groups showed that 1 month and 1 year after operation, the buccal soft tissue profile of the two groups were significantly higher than that before operation (P<0.05); and 1 year after operation, the buccal soft tissue profile of the two groups were significantly lower than 1 month after operation. Comparison between groups showed that 1 year after operation, the buccal soft tissue profile of the experimental group was significantly higher than that of the control group (P<0.05). Comparison within groups showed that 1 month and 1 year after surgery, the thickness of the buccal soft tissue of the two groups were significantly higher than that before surgery. Comparison between groups showed that there was no significant difference in the thickness of the buccal soft tissue at each time point between the two groups(P>0.05). Comparison within groups showed that 1 month and 1 year after operation, the red aesthetic scores of patients in the two groups were significantly higher than those before operation (P<0.05); in addition, the red aesthetic scores of the two groups 1 year after surgery were significantly higher than those 1 month after surgery (P<0.05). Comparison between groups showed that 1 month and 1 year after surgery, the red aesthetic scores of the experimental group were significantly higher than those of the control group (P<0.05). One year after operation, the success rate of the experimental group and the control group were 90.24% (37/41) and 85.37% (35/41), respectively, and there was no significant difference between the two groups(P>0.05). There was no significant difference in the incidence of complications between the two groups(P>0.05). CONCLUSIONS: Autologous dentin particles can be used to guide bone regeneration to treat patients with periodontal bone defects, which can reduce the depth of periodontal pockets and loss of attachment, improve the buccal soft tissue profile and red aesthetic score, and the success rate is equivalent to implantation of Bio-Oss. Therefore, it is a safe and reliable treatment method. Reference | Related Articles | Metrics

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Study of the morphological difference of upper jaw impressions taken by intra-oral digital scanning, functional impression technique PENG Yan, WU Xue -ying, ZHONG Qun, FANG Shuo-bo, YE Ye Shanghai Journal of Stomatology    2022, 31 (5): 512-516.   DOI: 10.19439/j.sjos.2022.05.012 Abstract （360）      PDF （834KB）（448）       Knowledge map    PURPOSE: To compare the difference in distal-extension models taken by intra-oral digital scanning and functional impression technique. METHODS: Twenty patients with dentition defect in upper jaw, including 10 Kennedy Ⅰ defect and another 10 Kennedy Ⅱ defect, were chosen to treat with intraoral digital scanning and functional impression technique respectively. Laboratory cast scanner was used to scan the plaster casts made from the functional impression to obtain the three-dimensional data. All the data were stored in STL format. The 3D data collecting from intra-oral digital scanning and functional impression from the same patient were compared by Geomagic Qualify 12 software, and the trueness of the difference on free end morphology were analyzed. Paired t test was used to analyze the difference of the alveolar mucosa variable with SPSS 20.0 software package. RESULTS: There was no significant difference between the group of Kennedy I defect and Kennedy Ⅱ defect(P>0.05） regarding the two methods of impression. All the cases presented the tendency that with distance from distal teeth increasing, the average RMS also increased. CONCLUSIONS: Whether using intra-oral digital scanning or functional impression technique, the type of dentition defect was not obviously related to the difference of RMS in distal-extension tissues. In addition, there is positive correlation between the length of distal-extension edentulous area and difference of RMS, which needs further studies with more cases. Reference | Related Articles | Metrics

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Effect of keratinized gingival width on periodontal regenerative surgery for the treatment of intrabony defects: a retrospective study LIU Hao-hao, HU Yi, ZHANG Chen, CHEN Mei-hua Shanghai Journal of Stomatology    2022, 31 (5): 507-511.   DOI: 10.19439/j.sjos.2022.05.011 Abstract （406）      PDF （1199KB）（437）       Knowledge map    PURPOSE: To evaluate the effect of keratinized tissue width (KTW) on periodontal regenerative surgery for the treatment of intrabony defects. METHODS: The clinical data of 14 patients (44 intrabony defect sites) treated with periodontal regenerative surgery were retrospectively analyzed at baseline and 2-year of follow-up. Forty four sites were divided into KTW2 mm group and KTW≤2 mm group according to KTW at baseline. Periodontal clinical indicators of the 2 groups were analyzed by SPSS 25.0 software package. RESULTS: At 2-year post-treatment, probing depth (PD) and clinical attachment loss (CAL) in the 2 groups were decreased significantly compared with those at baseline(P<0.05). There was no significant difference in ΔPD, but △CAL in KTW2 mm group was significantly greater than that in KTW≤2 mm group. CONCLUSIONS: Periodontal regenerative surgery for the treatment of intrabony defects can effectively reduce periodontal pocket inflammation, decrease periodontal pocket depth and increase the level of attachment. When the width of the keratinized gingiva is insufficient(KTW≤2 mm), the regeneration of the attachment level obtained by surgery is limited, and the efficacy of regenerative surgery is poor. Reference | Related Articles | Metrics

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Efficiency of clear aligners in extrusion of posterior teeth evaluated by3-dimensional model superimposition ZHAO Jia-yuan, HU Cheng-si, JIN Yu, ZHANG Ling-ling, YI Fang, CHEN Jun-jie, ZHOU Ping, LU Yan-qin, LI Cheng-ri Shanghai Journal of Stomatology    2022, 31 (5): 556-560.   DOI: 10.19439/j.sjos.2022.05.020 Abstract （369）      PDF （1209KB）（437）       Knowledge map    PURPOSE: To explore the efficiency of posterior teeth extrusion with clear aligners by 3-dimensional model superimposition, which provides a reference for the design of clinical programs. METHODS: We selected 24 patients with clear aligners whose posterior teeth were designed to extend more than 0.5 mm, and a total of 126 teeth were included. Digital models were obtained before and after treatment by intraoral scanning with iTero, named as "actual initial" and "achieved" digital models. Initial and final models from the ClinCheck, labeled as "virtual initial" and "predicted" models respectively. Initial, predicted, and achieved digital dental models were exported as stereolithography files and subsequently imported into Geomagic Studio. Extrusive measurements were made from the superimposition of the initial and predicted models (predicted movement) and from the superimposed initial and achieved models (achieved movement). Statistical analysis was performed with SPSS 23.0 and GraphPad Prism 5.0 software package. The extrusion efficiency of the posterior teeth was calculated, at the meanwhile the influencing factors were analyzed. RESULTS: The mean extrusion efficiency of posterior teeth during clear aligners treatment was 30.2%. The actual extrusion was linearly related to the expected(P<0.05), and the linear regression equation was y=0.305x-0.010. The difference between the actual and the predicted extrusion was positively correlated with the number of appliances(P<0.05), and also positively correlated with the predicted extrusion value(P<0.001). This difference was larger in low-angle patients than in patients with average angle (P<0.05). CONCLUSIONS: The extrusion rate of posterior teeth is relatively low during clear aligners treatment, and the average efficiency is 30.2%. Vertical growth pattern affects the extrusion efficiency. So more overcorrections can be designed for average angle patients. Reference | Related Articles | Metrics

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Analysis of potential pathogenic factors of trigeminal neuralgia in rats LIU Yue-min, CHAI Ying, WEI Wen-bin, LIU Zhi-yang, HAN Zi-xiang, CHEN Min-jie Shanghai Journal of Stomatology    2023, 32 (1): 33-39.   DOI: 10.19439/j.sjos.2023.01.007 Abstract （306）      PDF （1559KB）（429）       Knowledge map    PURPOSES: Transcriptomics-based analysis of key transcriptional molecules in the pathogenesis of trigeminal neuropathic pain was conducted to screen key molecules in the pathogenesis of trigeminal neuralgia. METHODS: Rat trigeminal nerve pathological pain model, namely chronic constriction injury of distal infraorbital nerve (IoN-CCI), was constructed and animal behaviors postsurgery were observed and analyzed. Trigeminal ganglia were collected for RNA-seq transcriptomics analysis. StringTie was used to annotate and quantify genome expression. DESeq2 was applied to compare between groups with P value less than 0.05 and fold change greater than 2 times and less than 0.5 times to screen differential genes, and display them with volcano graphs and cluster graphs. ClusterProfiler software was used to perform GO function enrichment analysis of differential genes. RESULTS: On the fifth postoperative day (POD5), the rat's face-grooming behavior increased to a peak; on the seventh postoperative day (POD7), the von-frey value dropped to the lowest value, indicating that the mechanical pain threshold of rats was significantly decreased. RNA-seq analysis of IoN-CCI rat ganglia found that the significantly up-regulated signaling pathways included B cell receptor signaling pathway, cell adhesion, complement and coagulation cascade pathways; significantly down-regulated pathways were related to systemic lupus erythematosus. Multiple genes among Cacna1s, Cox8b, My1, Ckm, Mylpf, Myoz1, Tnnc2 were involved in mediating the occurrence of trigeminal neuralgia. CONCLUSIONS: B cell receptor signaling pathway, cell adhesion, complement and coagulation cascade pathways, neuroimmune pathways are closely related to the occurrence of trigeminal neuralgia. The interaction of multiple genes among Cacna1s, Cox8b, My11, Ckm, Mylpf, Myoz1, Tnnc2 leads to the occurrence of trigeminal neuralgia. Reference | Related Articles | Metrics

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A correlation study between the occlusal cant and temporomandibular joint space in patients with mandibular lateral deviation LI Shuang, XIAO Rong-rong, WANG Ming-feng Shanghai Journal of Stomatology    2024, 33 (3): 318-323.   DOI: 10.19439/j.sjos.2024.03.019 Abstract （267）      PDF （1159KB）（429）       Knowledge map    PURPOSE: To investigate the correlation between the occlusal canting and the bilateral temporomandibular joint (TMJ) space in adult and juvenile mandibular deviation patients and study the mutual influence between the occlusal canting and mandibular position, in order to provide references for clinical treatment. METHODS: CBCT data of mandibular deviation patients（20 adults,20 juveniles）were selected. Inivo5 Dental Anatomage software was used to reconstruct the structures. The occlusal cant and vertical height of the bilateral maxillary from canines to first molars were measured, and the vertical heights difference between the same teeth on both sides was calculated. The anterior, superior and posterior space of temporomandibular joint were measured respectively in both groups. Pearson correlation analysis between the occlusal canting and bilateral condylar space was carried out by using SPSS 17.0 software package. RESULTS: In the juvenile group, negative correlations were found between the occlusal cant and the superior TMJ space on the deviated side (P<0.05). Negative correlation was found between the vertical height difference of bilateral canines and the anterior TMJ space on the deviated side in the juvenile group(P<0.05). In the adult group, no significant correlation was observed among those correlated examination (P>0.05). CONCLUSONS: The occlusal canting is moderately correlated with mandibular position in the early stage of mandibular deviation patients. Early treatment of mandibular deviation is of great importance in preventing its progression into severe skeletal malocclusion, and more attention should be paid on the correction of the canted frontal occlusion plane. Reference | Related Articles | Metrics