牙龈卟啉单胞菌和肿瘤相关巨噬细胞的表达水平与口腔鳞癌临床预后的相关性分析

doi:10.19439/j.sjos.2025.02.004

上海口腔医学 ›› 2025, Vol. 34 ›› Issue (2): 132-138.doi: 10.19439/j.sjos.2025.02.004

牙龈卟啉单胞菌和肿瘤相关巨噬细胞的表达水平与口腔鳞癌临床预后的相关性分析

李慕秋¹, 李晨曦^1,*, 魏巍¹, 陈程², 王冰¹, 龚忠诚^1,*, 郭伟³, 任国欣³

1.新疆医科大学第一附属医院（附属口腔医院）口腔颌面肿瘤外科, 新疆维吾尔自治区口腔医学研究所,新疆乌鲁木齐 830054;
2.新疆大学软件学院,新疆乌鲁木齐 830046;
3.上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院, 国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室, 上海市口腔医学研究所,上海 200011

收稿日期:2024-05-27 修回日期:2024-07-10 出版日期:2025-04-25 发布日期:2025-05-15
通讯作者: 龚忠诚,E-mail: gzc740904@xjmu.edu.cn;李晨曦,E-mail: lichenximed@163.com。^*共同通信作者
作者简介:李慕秋（1995-）,男,硕士研究生,E-mail: 1574617946@qq.com
基金资助:
国家自然科学基金（82360481）; 口腔颌面发育与再生湖北省重点实验室开放课题基金（2022kqhm008）; 新疆维吾尔自治区科研创新项目（XJ2023G174,XJ2024G176）

Study on the correlation between the expression of Porphyromonas gingivalis, tumor-associated macrophages and the clinical prognosis of oral squamous cell carcinoma

Li Muqiu¹, Li Chenxi¹, Wei Wei¹, Chen Cheng², Wang Bing¹, Gong Zhongcheng¹, Guo Wei³, Ren Guoxin³

1. Department of Oral and Maxillofacial Oncology & Surgery, the First Affiliated Hospital(Affiliated Stomatological Hospital) of Xinjiang Medical University, Stomatological Research Institute of Xinjiang Uygur Autonomous Region. Urumqi 830054, Xinjiang Uygur Autonomous Region;
2. College of Software Engineering, Xinjiang University. Urumqi 830046, Xinjiang Uygur Autonomous Region;
3. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2024-05-27 Revised:2024-07-10 Online:2025-04-25 Published:2025-05-15

摘要/Abstract

摘要： 目的：探讨口腔鳞癌（oral squamous cell carcinoma,OSCC）肿瘤微环境中牙龈卟啉单胞菌（Porphyromonas gingivalis,P. gingivalis）和肿瘤相关巨噬细胞（tumor-associated macrophage,TAM）的表达水平及其临床意义。方法：选取新疆医科大学第一附属医院2008年6月—2020年6月收治的200例OSCC患者并收集相关临床资料,按照免疫组织化学（immunohistochemistry,IHC）染色结果分为P. gingivalis强表达组（n=139）和弱表达组（n=61）。IHC技术分别定量2组标本DOK3及TAM的表达量。比较2组患者三者表达水平与各临床病理指标之间的相关性及其对患者预后的影响。采用R软件4.2.2对数据进行统计学分析。结果：患者死亡、临床晚期、T3~T4期、发生区域淋巴结转移、肿瘤≥3 cm及中低分化、复发与P. gingivalis高水平表达呈显著相关（P<0.05）。DOK3的强阳性表达与P. gingivalis高水平表达患者的年龄、生存状态、病理T分期、N分期及肿瘤复发显著相关（P<0.05）;而TAM的强阳性表达则与T分期、临床分期和肿瘤分化程度显著相关（P<0.05）。此外,P. gingivalis的表达与DOK3及TAM的表达呈显著正相关（P<0.01）。P. gingivalis、DOK3、TAM强阳性表达的OSCC患者的预后较弱表达的患者差（P<0.05）。结论：P. gingivalis的免疫表达水平与DOK3及TAM呈正相关。三者的高表达水平与OSCC患者不良预后有关,临床上可考虑作为评价患者预后的标志物。

关键词: 口腔鳞癌, 牙龈卟啉单胞菌, 免疫微环境, 肿瘤相关巨噬细胞, 临床预后

Abstract: PURPOSE: To explore the immunoexpression and clinical significance regarding Porphyromonas gingivalis (P. gingivalis) and tumor-associated macrophage (TAM) in the tumor microenvironment of oral squamous cell carcinoma (OSCC). METHODS: A total of 200 OSCC patients admitted to the First Affiliated Hospital of Xinjiang Medical University between June 2008 and June 2020 were divided into P.gingivalis-strong-expression group(n=139) and P. gingivalis-weak-expression group (n=61), based on the immunohistochemical(IHC) staining. The detection of DOK3 and TAM was performed to quantify their expression level in two groups, respectively. The associations between the three mentioned biomarkers and clinicopathologic indicators were compared between the two groups, and the impact on patients' prognosis was also analyzed. R 4.2.2 software was used for data analysis. RESULTS: Death, late-stage cancer, T3-T4 and N(+) stage, large size (≥3 cm), moderate and poor differentiation, and recurrence exhibited significant associations with strong immunoexpression of P. gingivalis(P<0.05). Several variables of OSCC patients with strong immunoexpression of P. gingivalis including age, survival status, T and N stage, recurrence exhibited significant associations with strong immunoexpression of DOK3 (P<0.05); whereas T stage, clinical stage and tumor differentiation exhibited significant associations with strong immunoexpression of TAM (P<0.05). Additionally, the immunoexpression of P. gingivalis was positively associated with the immunoexpression of DOK3 and TAM(P<0.01). The prognosis of OSCC patients with strong positive expression of P. gingivalis, DOK3 and TAM was worse than that of patients with weak immunoexpression, respectively(P<0.05). CONCLUSIONS: P. gingivalis is positively correlated with DOK3 and TAM. In addition, the strong immunoexpression levels of P. gingivalis, DOK3 and tumor-associated macrophage are associated with poor prognosis in patients with OSCC. Collectively, the mentioned three biomarkers can be considered as important indicators for evaluating clinical prognosis.

Key words: Oral squamous cell carcinoma, Porphyromonas gingivalis, Immune microenvironment, Tumor-associated macrophage, Clinical prognosis

中图分类号:

R739.8

李慕秋, 李晨曦, 魏巍, 陈程, 王冰, 龚忠诚, 郭伟, 任国欣. 牙龈卟啉单胞菌和肿瘤相关巨噬细胞的表达水平与口腔鳞癌临床预后的相关性分析[J]. 上海口腔医学, 2025, 34(2): 132-138.

Li Muqiu, Li Chenxi, Wei Wei, Chen Cheng, Wang Bing, Gong Zhongcheng, Guo Wei, Ren Guoxin. Study on the correlation between the expression of Porphyromonas gingivalis, tumor-associated macrophages and the clinical prognosis of oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2025, 34(2): 132-138.

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牙龈卟啉单胞菌和肿瘤相关巨噬细胞的表达水平与口腔鳞癌临床预后的相关性分析

Study on the correlation between the expression of Porphyromonas gingivalis, tumor-associated macrophages and the clinical prognosis of oral squamous cell carcinoma

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