LINC 00478在口腔鳞癌中的表达及对细胞增殖、迁移、侵袭的影响

doi:10.19439/j.sjos.2025.02.003

上海口腔医学 ›› 2025, Vol. 34 ›› Issue (2): 126-131.doi: 10.19439/j.sjos.2025.02.003

LINC 00478在口腔鳞癌中的表达及对细胞增殖、迁移、侵袭的影响

赵谦^1,2,*, 韩林孜^3,*, 赵璐⁴, 王莉莉⁵, 庄立琨⁵, 袁荣涛⁴

1.青岛大学口腔医学院,山东青岛 266003;
2.临沂市中心医院口腔科,山东临沂 276401;
3.青岛大学附属青岛市口腔医院,山东青岛 266001;
4.青岛市市立医院（康复大学青岛医院）口腔医学中心,5.中心实验室,山东青岛 266071

收稿日期:2024-01-26 修回日期:2024-03-07 出版日期:2025-04-25 发布日期:2025-05-15
通讯作者: 袁荣涛,E-mail: yuanrongtao@163.com
作者简介:赵谦（1998-）,男,硕士研究生,E-mail: m17865788280@163.com;韩林孜（1995-）,男,医师,E-mail: 351544159@qq.com。^*并列第一作者

Expression of LINC 00478 in oral squamous cell carcinoma and its effect on proliferation, migration and invasion

Zhao Qian^1,2, Han Linzi³, Zhao Lu⁴, Wang Lili⁵, Zhuang Likun⁵, Yuan Rongtao⁴

1. School of Stomatology, Qingdao University. Qingdao 266003;
2. Department of Stomatology, Linyi Central Hospital. Linyi 276401;
3. Qingdao Stomatological Hospital Affiliated to Qingdao University. Qingdao 266001;
4. Center of Stomatology, 5. Central Lab, Qingdao Municipal Hospital. Qingdao 266071, Shandong Province, China

Received:2024-01-26 Revised:2024-03-07 Online:2025-04-25 Published:2025-05-15

摘要/Abstract

摘要： 目的：探讨LINC 00478在口腔鳞癌（oral squamous cell carcinoma,OSCC）中的表达差异,分析LINC 00478与OSCC患者预后的关系。探讨LINC 00478对OSCC增殖、迁移和侵袭的影响,为OSCC的分子检测与临床诊疗提供新的潜在方案。方法：通过TCGA数据库下载OSCC转录组数据样本信息,使用R软件研究OSCC肿瘤组织与正常组织中LINC 00478的表达差异;分析LINC 00478表达与OSCC患者预后的关系。收集26例OSCC患者的癌及癌旁组织样本,通过RT-PCR检测OSCC样本中LINC 00478的表达,明确LINC 00478在OSCC组织中的差异表达。体外实验研究LINC 00478对CAL27细胞增殖、侵袭、迁移的影响,利用CCK-8实验观察LINC 00478对CAL27增殖能力的影响,Transwell实验检测LINC 00478对CAL27迁移和侵袭的影响。采用GraphPad Prism 8.0.2软件对数据进行统计学分析。结果：TCGA数据库结果显示,LINC 00478在OSCC组织中的表达显著低于癌旁组织(P<0.001),且其高表达代表LINC 00478与患者预后较好相关（P<0.05）。CCK-8结果显示,过表达LINC 00478可显著抑制CAL27的增殖能力（P<0.05）。Transwell结果表明,过表达LINC 00478可显著抑制CAL27的迁移、侵袭能力(P<0.05)。结论：LINC 00478在OSCC中显著低表达,且其高表达与预后呈显著正相关。过表达LINC 00478基因将抑制CAL27的增殖、迁移及侵袭能力,在OSCC中可能发挥抑癌作用。

关键词: LINC 00478, 口腔鳞癌, 增殖, 侵袭, 迁移

Abstract: PUEPOSE: To examine the expression difference of LINC 00478 in oral squamous cell carcinoma (OSCC) and analyze its relationship with the prognosis of OSCC patients. To investigate the effects of LINC 00478 on proliferation, migration, and invasion of OSCC cells, with the goal of offering a new potential approach for molecular detection and clinical diagnosis of OSCC. METHODS: Transcriptome data samples from OSCC were obtained from TCGA, and R software was utilized to assess the expression variance of LINC 00478 in OSCC tumor and normal tissues. Tissue samples from 26 OSCC patients, including cancer and adjacent tissues, were collected for RT-PCR analysis to determine the differential expression of LINC 00478 in OSCC tissues. In vitro experiments were conducted to explore the impact of LINC 00478 on proliferation, invasion, and migration of CAL27 cells. CCK-8 assay was used to observe the proliferation capability of LINC 00478 in CAL27 cells, while Transwell experiment was employed to assess its effects on cell migration and invasion. GraphPad Prism 8.0.2 software was used for statistical analysis of the data. RESULTS: Analysis of the TCGA database revealed that LINC 00478 was weakly expressed in OSCC tissues compared to adjacent normal tissues (P<0.001), with high-expression LINC 00478 correlating with improved patient prognosis(P<0.05). Furthermore, LINC 00478 was significantly under-expressed in 26 OSCC tissues (P<0.001). Experimental data from CCK-8 assays indicated that upregulation of LINC 00478 suppressed CAL27 proliferation (P<0.05). Additionally, Transwell experiments demonstrated that high-expression LINC 00478 significantly inhibited CAL27 migration and invasion (P<0.05). CONCLUSIONS: LINC 00478 is significantly under-expressed in OSCC, and its high expression is positively correlated with a better prognosis. Over-expression of the LINC 00478 gene has been shown to inhibit the proliferation, migration, and invasion of CAL27 cells, suggesting a potential role in inhibiting cancer progression in OSCC.

Key words: LINC 00478, Oral squamous cell carcinoma, Proliferation, Invasion, Migration

中图分类号:

R739.8

赵谦, 韩林孜, 赵璐, 王莉莉, 庄立琨, 袁荣涛. LINC 00478在口腔鳞癌中的表达及对细胞增殖、迁移、侵袭的影响[J]. 上海口腔医学, 2025, 34(2): 126-131.

Zhao Qian, Han Linzi, Zhao Lu, Wang Lili, Zhuang Likun, Yuan Rongtao. Expression of LINC 00478 in oral squamous cell carcinoma and its effect on proliferation, migration and invasion[J]. Shanghai Journal of Stomatology, 2025, 34(2): 126-131.

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LINC 00478在口腔鳞癌中的表达及对细胞增殖、迁移、侵袭的影响

Expression of LINC 00478 in oral squamous cell carcinoma and its effect on proliferation, migration and invasion

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