新型生物活性玻璃对乳牙根尖周炎常见细菌抗菌效果评价

doi:10.19439/j.sjos.2024.01.008

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (1): 49-53.doi: 10.19439/j.sjos.2024.01.008

新型生物活性玻璃对乳牙根尖周炎常见细菌抗菌效果评价

方颖¹, 黄文燕¹, 苗国厚², 孟斯¹, 彭博¹, 曾素娟¹

1.广州医科大学附属口腔医院儿童口腔科,广东省口腔组织修复与重建工程技术研究中心, 广州市口腔再生医学基础与应用研究重点实验室,广东广州 510182;
2.广州医科大学附属口腔医院,广东省口腔组织修复与重建工程技术研究中心, 广州市口腔再生医学基础与应用研究重点实验室,广东广州 510182

收稿日期:2023-08-07 修回日期:2023-09-20 出版日期:2024-02-25 发布日期:2024-03-07
通讯作者: 曾素娟,E-mail：Zengsujuan@foxmai.com
作者简介:方颖（1989-）,女,硕士研究生,主治医师,E-mail: fangy_2639@163.com
基金资助:
广州市卫生健康科技项目（20221A010064）

Study on the antimicrobial effect of new bioactive glass against common bacteria in apical periodontitis of deciduous teeth

FANG Ying¹, HUANG Wen-yan¹, MIAO Guo-hou², MENG Si¹, PENG bo¹, ZENG Su-juan¹

1. Department of Pediatric Dentistry, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine. Guangzhou 510182;
2. Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine. Guangzhou 510182, Guangdong Province, China

Received:2023-08-07 Revised:2023-09-20 Online:2024-02-25 Published:2024-03-07

摘要/Abstract

摘要： 目的: 研究不同浓度新型生物活性玻璃（bioactive glass,BG）对乳牙根尖周炎常见细菌的抗菌效果。方法: 采用纸片扩散法检测并观察新型BG处理粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌后形成的抑菌环直径（mm）,同时测定粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌的最小抑菌浓度（minimal inhibitory concentration,MIC）、最小杀菌浓度（minimal bactericidal concentration,MBC）和最小菌斑清除浓度（minimal biofilm eradication concentration,MBEC）,20、40、60、80 mg/mL 新型BG处理3种菌的混合菌斑24 h,于激光共聚焦显微镜（confocal laser scanning microscopy,CLSM）下摄片观察新型BG对混合菌斑的抑菌效果。采用GraphPad Prism 10.0软件对数据进行统计学分析。结果: 新型BG对乳牙根尖周炎常见细菌显示出很强的抗菌潜力;新型BG对菌斑的 MBEC 显著大于对粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌的 MIC 和 MBC。随着新型BG浓度加大,混合菌斑死菌数量显著增加,与空白对照组有显著差异（P<0.05）。结论: 新型BG对乳牙根尖周炎常见细菌粪肠球菌、牙龈卟啉单胞菌和具核梭杆菌具有显著抗菌功效。

关键词: 生物活性玻璃, 粪肠球菌, 牙龈卟啉单胞菌, 具核梭杆菌, 抗菌效果

Abstract: PURPOSE: To study the antimicrobial effect of different concentrations of new bioactive glass(BG) on common bacteria in apical periodontitis of deciduous teeth. METHODS: The diameter (mm) of the inhibitory rings formed after treatment of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum with the new bioactive glass was detected and observed by paper diffusion method, and the minimal inhibitory concentration(MIC), minimal bactericidal concentration (MBC) and minimal biofilm eradication concentration (MBEC) of E. faecalis, P. gingivalis and C. pseudomallei were determined. The mixed plaques of the three bacteria were treated with 20, 40, 60 and 80 mg/mL of the new bioactive glass for 24 h. The results were analyzed by laser confocal microscopy. The antibacterial effect of the new bioactive glass on the mixed plaque was observed by confocal laser scanning microscopy (CLSM). Statistical analysis was performed with GraphPad Prism 10.0 software. RESULTS: The new bioactive glass showed strong antibacterial potential against the common bacteria of apical periodontitis; the MBEC of the new bioactive glass on the plaque was significantly greater than MIC and MBC of Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, and as the concentration of the new bioactive glass increased, the number of dead bacteria in the mixed plaque increased, and there was significant difference from that of the blank control group (P<0.05). CONCLUSIONS: The novel bioactive glass shows significant antibacterial efficacy against Enterococcus faecalis, Porphyromonas gingivalis and Clostridium nucleatum, which are the common bacteria in apical periodontitis of deciduous teeth.

Key words: Bioactive glass, Enterococcus faecalis, Porphyromonas gingivalis, Clostridium perfringens, Antibacterial effect

中图分类号:

R788.2

方颖, 黄文燕, 苗国厚, 孟斯, 彭博, 曾素娟. 新型生物活性玻璃对乳牙根尖周炎常见细菌抗菌效果评价[J]. 上海口腔医学, 2024, 33(1): 49-53.

FANG Ying, HUANG Wen-yan, MIAO Guo-hou, MENG Si, PENG bo, ZENG Su-juan. Study on the antimicrobial effect of new bioactive glass against common bacteria in apical periodontitis of deciduous teeth[J]. Shanghai Journal of Stomatology, 2024, 33(1): 49-53.

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新型生物活性玻璃对乳牙根尖周炎常见细菌抗菌效果评价

Study on the antimicrobial effect of new bioactive glass against common bacteria in apical periodontitis of deciduous teeth

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