人β防御素在牙髓组织中的表达及与炎症因子的关系

doi:10.19439/j.sjos.2017.04.004

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (4): 368-373.doi: 10.19439/j.sjos.2017.04.004

人β防御素在牙髓组织中的表达及与炎症因子的关系

翟越¹, 黄枧英², 朴玄¹, 纪芳³, 费照亮⁴, 陶疆¹

1．上海交通大学医学院附属第九人民医院·口腔医学院口腔综合科,上海市口腔医学重点实验室,上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011；
2.江西省妇幼保健院新生儿疾病筛查中心中心实验室, 江西南昌 330002；
3.上海交通大学医学院附属第九人民医院·口腔医学院口腔正畸科,上海市口腔医学重点实验室, 上海市口腔医学研究所,国家口腔疾病临床研究中心,上海 200011；
4.中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海 200031

收稿日期:2017-02-15 修回日期:2017-04-06 出版日期:2017-08-25 发布日期:2017-09-01
通讯作者: 陶疆, E-mail: taojiang_doctor@hotmail.com
作者简介:翟越（1991-）,女,硕士,E-mail:zhaiyuekouqiang@yeah.net
基金资助:
国家自然科学基金（81200756、81500813）; 上海高校高峰高原学科建设项目

Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

ZHAI Yue¹, HUANG Jian-ying², HYUN Park¹, JI Fang³, FEI Zhao-liang⁴, TAO Jiang¹

1.Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine;Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011;
2.Central Laboratory, Center for Neonatal Disease Screening, Maternal and Child Health Hospital of Jiangxi Province. Nanchang 330002, Jiangxi Province;
3.Department of Orthodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai 200011;
4.Institute of Biochemistry and Cell Biology,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences.Shanghai 200031, China

Received:2017-02-15 Revised:2017-04-06 Online:2017-08-25 Published:2017-09-01

摘要/Abstract

摘要： 目的探讨人β防御素（human β-defensin, HBD）家族成员在人牙髓组织中的表达,以HBD2为代表,探讨HBD在牙髓炎症反应中所受调控及HBD家族成员之间的调节关系。方法在NCBI GEO Profiles数据库中检索HBD在人牙髓组织中表达的基因芯片信息,并运用反转录聚合酶链式反应（RT-PCR）进行验证。运用4种炎症因子TNF-α、IL-1α、IL-1β和IL-6不同组合刺激人牙髓细胞,采用实时荧光定量核酸扩增检测法（qPCR）检测相应组分对HBD2表达水平的影响;HBD110预处理及LPS刺激牙髓细胞后,采用qPCR方法检测TNF-α、IL-1α和HBD2的表达水平。采用GraphPad Prism 5.01 对实验组和对照组的结果进行统计学分析。结果在NCBI GEO Profiles数据库中检索到27种HBD家族成员在人牙髓组织中表达。TNF-α、IL-1α、IL-1β和IL-6联合作用,可显著提升HBD2的表达水平。HBD110通过调控TNF-α和IL-1α的表达,提高HBD2的表达水平。结论除已有报道的HBD1、HBD2、HBD3和HBD4外,其他HBD家族成员在人牙髓组织中表达,且HBD2在牙髓组织中受炎症因子及其他HBD家族成员的调控。

关键词: 人β, 防御素, 牙髓, 炎症反应

Abstract: To investigate the expression of human β-defensin(HBD) in human dental pulp tissue and to explore the regulation of HBD in pulp inflammation and the relationship among HBD family members. METHODS: The gene expression of HBD in human dental pulp tissue was assessed in NCBI GEO profiles and was verified by RT-PCR. Human dental pulp cells were stimulated with TNF-α, IL-1α, IL-1β and IL-6 in different combinations and the expression of HBD2 was analyzed by qPCR. Human dental pulp cells were pretreated with HBD110 and then stimulated with LPS and the expression of TNF-α,IL-1α and HBD2 were analyzed by qPCR. GraphPad Prism 5.01 was used to analyze the results of the experimental and the control groups. RESULTS: 27 HBDs were found to express in human dental pulp tissue in NCBI GEO Profiles. The joint overexpression of TNF-α, IL-1α, IL-1β and IL-6 increased the expression of HBD2; HBD110 increased the expression of HBD2 by increasing the expression of TNF-α and IL-1α. CONCLUSIONS: Many other HBDs have positive expression in human dental pulp issue besides of HBD1, HBD2, HBD3, HBD4 and the inflammation factors and other HBDs can regulate the expression of HBD2 in dental pulp.

Key words: Human β, -defensin, Dental pulp, Inflammation

中图分类号:

R781.3

翟越, 黄枧英, 朴玄, 纪芳, 费照亮, 陶疆. 人β防御素在牙髓组织中的表达及与炎症因子的关系[J]. 上海口腔医学, 2017, 26(4): 368-373.

ZHAI Yue, HUANG Jian-ying, HYUN Park, JI Fang, FEI Zhao-liang, TAO Jiang. Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue[J]. Shanghai Journal of Stomatology, 2017, 26(4): 368-373.

参考文献

[1] Pazgier M, Hoover DM, Yang D, et al. Human beta-defensins [J]. Cell Mol Life Sci, 2006, 63(11): 1294-1313.
[2] Joly S, Maze C, Mccray PB, Jr, et al. Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms [J]. J Clin Microbiol, 2004, 42(3): 1024-1029.
[3] Wilson SS, Wiens ME, Smith JG. Antiviral mechanisms of human defensins [J]. J Mol Biol, 2013, 425(24): 4965-4980.
[4] Schneider JJ, Unholzer A, Schaller M, et al. Human defensins [J]. J Mol Med (Berl), 2005, 83(8): 587-595.
[5] Matsuzaki K. Why and how are peptide-lipid interactions utilized for self-defense? Magainins and tachyplesins as archetypes [J]. Biochim Biophys Acta, 1999, 1462(1-2): 1-10.
[6] Boniotto M, Jordan WJ, Eskdale J, et al. Human beta-defensin 2 induces a vigorous cytokine response in peripheral blood mononuclear cells[J]. Antimicrob Agents Chemother, 2006, 50(4): 1433-1441.
[7] Kobayashi GS, Alvizi L, Sunaga DY, et al. Susceptibility to DNA damage as a molecular mechanism for non-syndromic cleft lip and palate [J]. PLoS One, 2013, 8(6): e65677.
[8] Dommisch H, Winter J, Acil Y, et al. Human beta-defensin (hBD-1, -2) expression in dental pulp[J]. Oral Microbiol Immunol, 2005, 20(3): 163-166.
[9] 王丽丽. 人β防御素在牙髓组织中的免疫组织化学研究[D]. 山东: 山东大学, 2011.
[10] Dommisch H, Winter J, Willebrand C, et al. Immune regulatory functions of human beta-defensin-2 in odontoblast-like cells [J]. Int Endod J, 2007, 40(4): 300-307.
[11] Lee SI, Kang SK, Jung HJ, et al. Muramyl dipeptide activates human beta defensin 2 and pro-inflammatory mediators through Toll-like receptors and NLRP3 inflammasomes in human dental pulp cells [J]. Clin Oral Investig, 2015, 19(6): 1419-1428.
[12] Hazlett L, Wu M. Defensins in innate immunity [J]. Cell Tissue Res, 2011, 343(1): 175-188.
[13] Baik JE, Ryu YH, Han JY, et al. Lipoteichoic acid partially contributes to the inflammatory responses to Enterococcus faecalis [J]. J Endod, 2008, 34(8): 975-982.
[14] Lee SI, Min KS, Bae WJ, et al. Role of SIRT1 in heat stress- and lipopolysaccharide-induced immune and defense gene expression in human dental pulp cells [J]. J Endod, 2011, 37(11): 1525-1530.
[15] Kim YS, Min KS, Lee SI, et al. Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells [J]. J Endod, 2010, 36(1): 64-69.

人β防御素在牙髓组织中的表达及与炎症因子的关系

Expression of human β-defensin and its relationship with inflammatory factor in human dental pulp tissue

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	姚旭飞, 蓝博, 季育, 周雪君. 微创环切术对牙列缺损种植患者炎症反应及牙槽嵴顶骨吸收的影响[J]. 上海口腔医学, 2024, 33(4): 398-402.
[2]	王静, 徐娜, 任慧迪. TNF-α通过ERK1/2-Runx2信号通路调控SHED成骨分化能力的实验研究[J]. 上海口腔医学, 2024, 33(2): 135-140.
[3]	刘昭辰, 孙培, 潘克清, 王培彦, 张慧, 袁昌青, 邓婧. 次氯酸溶液的稳定性及对口腔组织刺激性的体外研究[J]. 上海口腔医学, 2024, 33(2): 141-147.
[4]	潘耀耀, 马晓楠, 陈呈. iRoot BP plus对成人龋源性露髓治疗疗效及对牙髓血流的影响[J]. 上海口腔医学, 2024, 33(2): 160-163.
[5]	江煜, 石磊, 蔡佳. 超声冲洗结合氯己定在牙髓炎根管治疗中的应用效果评价[J]. 上海口腔医学, 2024, 33(2): 170-174.
[6]	张安妮, 丁灿灿, 黄丽苹, 李适廷. 抑制连接蛋白43介导半通道活性促进脂多糖诱导的人牙髓细胞成牙本质分化[J]. 上海口腔医学, 2024, 33(1): 22-29.
[7]	刘岩, 单丹妮, 孙晶, 邹雨希, 袁长永. Let-7a对人牙髓干细胞增殖、凋亡和成骨分化的影响[J]. 上海口腔医学, 2023, 32(5): 468-474.
[8]	杨一凡, 骆春梅, 李小兵, 杜芹. 温度与辣椒素对大鼠口腔黏膜的刺激效应[J]. 上海口腔医学, 2023, 32(4): 363-368.
[9]	李宗谕, 李晓琳, 尉鹏功, 曲柳, 仇丽鸿, 于雅琼. 白藜芦醇在人牙髓干细胞成牙本质向分化中的调控机制研究[J]. 上海口腔医学, 2023, 32(2): 132-136.
[10]	胡逸鹏, 欧晓艳, 钟鸿梅. miR-124对牙髓间充质干细胞成骨分化的影响及机制探讨[J]. 上海口腔医学, 2022, 31(5): 466-470.
[11]	马智菲, 周梦琪, 王丽珍, 承清, 洪瑾. Er:YAG 激光在小型猪牙髓血运重建术中的应用效果评价[J]. 上海口腔医学, 2022, 31(4): 367-373.
[12]	王雪纯, 吴艳棋, 梅鹏, 张博钧, 朱敏. 低强度牵张应力对异丙肾上腺素诱导下牙周膜成纤维细胞炎症反应的影响[J]. 上海口腔医学, 2022, 31(3): 225-231.
[13]	付洪海, 孙乐刚, 丁昌成, 马向瑞, 黄玉梅. miR-31-5p对牙髓干细胞HIF-1α/BNIP3信号通路及成骨相关因子表达的影响[J]. 上海口腔医学, 2022, 31(3): 237-242.
[14]	林晓明, 苏江凌, 卜翠萍. 显微镜下血运重建术与根尖诱导成形术治疗恒牙牙髓坏死的疗效比较[J]. 上海口腔医学, 2022, 31(3): 318-321.
[15]	王瑞, 杨谛, 于雅琼, 郭佳杰, 仇丽鸿. 牙髓卟啉单胞菌内毒素对成骨细胞分化的抑制作用[J]. 上海口腔医学, 2021, 30(4): 350-354.