次氯酸溶液的稳定性及对口腔组织刺激性的体外研究

doi:10.19439/j.sjos.2024.02.006

上海口腔医学 ›› 2024, Vol. 33 ›› Issue (2): 141-147.doi: 10.19439/j.sjos.2024.02.006

次氯酸溶液的稳定性及对口腔组织刺激性的体外研究

刘昭辰^1,2, 孙培³, 潘克清³, 王培彦¹, 张慧³, 袁昌青³, 邓婧^2,3

1.青岛大学口腔医学院,山东青岛 266003;
2.青岛市口腔数字医学与3D打印工程实验室,山东青岛 266003;
3.青岛大学附属医院口腔内科,山东青岛 266003

收稿日期:2023-07-25 修回日期:2023-09-21 出版日期:2024-04-25 发布日期:2024-05-14
通讯作者: 邓婧,E-mail: dengjing3333@126.com
作者简介:刘昭辰（1997-）,女,在读硕士研究生,E-mail: chuangshao1997@163.com
基金资助:
青岛市口腔医学攀峰学科项目

An in vitro experiment on the stability and irritant of hypochlorous acid in oral cavity

LIU Zhao-chen^1,2, SUN Pei³, PAN Ke-qing³, WANG Pei-yan¹, ZHANG Hui³, YUAN Chang-qing³, DENG Jing^2,3

1. School of Stomatology, Qingdao University. Qingdao 266003;
2. Dental Digital Medicine & 3D Printing Engineering Laboratory of Qingdao. Qingdao 266003;
3. Department of Stomatology, Affiliated Hospital of Qingdao University. Qingdao 266003, Shandong Province, China

Received:2023-07-25 Revised:2023-09-21 Online:2024-04-25 Published:2024-05-14

摘要/Abstract

摘要： 目的: 研究不同次氯酸（hypochlorous acid, HClO）溶液在模拟临床使用条件下的理化性能及杀灭粪肠球菌的稳定性,并检测HClO对口腔软、硬组织和细胞的相容性。方法: 在模拟条件下监测2种不同生产工艺HClO样本的理化指标（有效氯浓度、氧化还原电位和pH值）变化,通过悬液定量杀菌试验测定不同浓度梯度HClO溶液的杀菌对数值。将牙髓组织、舌组织及牙本质块分别浸泡于PBS、100 ppm HClO、200 ppm HClO和3% NaClO 4组溶液中,用称重法和显微硬度仪检测其对软、硬组织的影响。通过CCK-8法检测HClO、NaClO及其10倍稀释液对人牙龈成纤维细胞的毒性作用。采用GraphPad PRIS 8.0软件对数据进行统计学分析。结果: 在模拟条件下,HClO溶液的有效氯浓度随时间缓慢衰减,1个月内衰减程度<20 ppm,pH值及氧化还原电位值相对稳定。各样本浓度衰减前后其杀菌对数值均>5.00,HClO处理后的软组织重量及牙本质显微硬度值无显著改变（P>0.05）,HClO及其10倍稀释液处理5 min的细胞活性远高于NaClO（P<0.001）。结论: HClO溶液的理化性能及杀菌稳定性良好,能满足临床需求。相比NaClO,HClO细胞毒性低、组织相容性好,有望成为牙髓再生领域安全高效的消毒产品。

关键词: 微酸性次氯酸水, 根管消毒, 稳定性, 组织溶解, 牙髓再生

Abstract: PURPOSE: To study the stability of physicochemical properties and sterilizing effect about two commercially available hypochlorous acid (HClO) products under simulated clinical conditions, and to evaluate the compatibility of HClO on soft and hard tissues and cells in oral cavity. METHODS: Samples of HClO solution with different production processes were prepared, to detect the changes of physicochemical indexes of each sample over time under simulated clinical conditions (shielded from light at 20-25 ℃, open the cover for 5 minutes every day), including free available chlorine, oxidation-reduction potential and pH. Through suspension quantitative germicidal test, the antibiosis-concentration curve of HClO solution was made, so as to calibrate the change of antibacterial ability of disinfectant with the decrease of available chlorine content during storage. Pulp, tongue and dentine were immersed in PBS, 100 ppm HClO, 200 ppm HClO and 3% NaClO. The influence on soft and hard tissues was evaluated by weighing method and microhardness test. The toxic effects of HClO, NaClO and their 10-fold diluent on human gingival fibroblasts were determined by CCK-8 cytotoxicity assay. GraphPad PRIS 8.0 software was used to analyze the data. RESULTS: Under simulated conditions, the free available chlorine (FAC) of HClO solution decayed with time, and the attenuation degree was less than 20 ppm within 1 month. The bactericidal effect of each HClO sample was still higher than 5log after concentration decay. There was no obvious dissolution and destruction to soft and hard tissues for HClO(P>0.05). The cell viability of HClO to human gingival fibroblast cells (HGFC) was greater than 80%, which was much higher than 3% NaClO (P<0.001). CONCLUSIONS: The bactericidal effect and stability of HClO solution can meet clinical needs, which has low cytotoxicity and good histocompatibility. It is expected to become a safe and efficient disinfection product in the field of living pulp preservation and dental pulp regeneration.

Key words: Slightly acidic hypochlorous acid, Root canal disinfection, Stability, Tissue dissolution, Pulp regeneration

中图分类号:

R783.1

刘昭辰, 孙培, 潘克清, 王培彦, 张慧, 袁昌青, 邓婧. 次氯酸溶液的稳定性及对口腔组织刺激性的体外研究[J]. 上海口腔医学, 2024, 33(2): 141-147.

LIU Zhao-chen, SUN Pei, PAN Ke-qing, WANG Pei-yan, ZHANG Hui, YUAN Chang-qing, DENG Jing. An in vitro experiment on the stability and irritant of hypochlorous acid in oral cavity[J]. Shanghai Journal of Stomatology, 2024, 33(2): 141-147.

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次氯酸溶液的稳定性及对口腔组织刺激性的体外研究

An in vitro experiment on the stability and irritant of hypochlorous acid in oral cavity

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