温度与辣椒素对大鼠口腔黏膜的刺激效应

doi:10.19439/j.sjos.2023.04.005

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (4): 363-368.doi: 10.19439/j.sjos.2023.04.005

温度与辣椒素对大鼠口腔黏膜的刺激效应

杨一凡¹, 骆春梅^2,3, 李小兵¹, 杜芹²

1.四川大学华西口腔医学院儿童口腔科早期矫治专科,四川成都 610041;
2.电子科技大学附属四川省人民医院口腔科,四川成都 610072;
3.遵义医科大学口腔医学院,贵州遵义 563003

收稿日期:2022-04-29 修回日期:2022-06-08 发布日期:2023-09-07
通讯作者: 杜芹,E-mail：dududicn@163.com
作者简介:杨一凡（1998-）,男,硕士,E-mail：2293494044@qq.com
基金资助:
四川省科技厅重点研发项目基金（2023NSFSC1998）; 云南白药健康基金

Experimental study on the stimulation effect of temperature and capsaicin on oral mucosa of rats

YANG Yi-fan¹, LUO Chun-mei^2,3, LI Xiao-bing¹, DU Qin²

1. Department of Early Orthodontics, West China College of Stomatology, Sichuan University. Chengdu610041, Sichuan Province;
2. Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science, Technology. Chengdu610072, Sichuan Province;
3. College of Dentistry, Zunyi Medical University. Zunyi563003, Guizhou Province, China

Received:2022-04-29 Revised:2022-06-08 Published:2023-09-07

摘要/Abstract

摘要： 目的研究不同温度与辣椒素对口腔黏膜形态及上皮层内炎症因子表达的影响,观察温度及辣椒素引起的口腔黏膜变化。方法使用25、45、55 ℃生理盐水与辣椒素（反式-8-甲基-N-香草基-6-壬烯酰胺）溶液分别刺激大鼠口腔黏膜4周,随后处死大鼠,观察口腔黏膜状态并取大鼠口腔黏膜制作石蜡切片,进行H-E染色与免疫组织化学染色。光镜下观察大鼠口腔黏膜上皮组织的炎症状态与形态变化,检测并记录炎症因子TNF-α、IL-6和IL-8的表达。采用SPSS 22.0软件包进行统计学分析,利用Graphpad Prism 8.0软件进行统计图表绘制。结果使用不同温度生理盐水与辣椒素溶液刺激大鼠口腔后,H-E染色显示,55 ℃组大鼠的口腔黏膜表现出不同程度的上皮形态紊乱和固有层炎细胞浸润。免疫组织化学染色结果显示,55 ℃生理盐水与辣椒素溶液会促进黏膜上皮内IL-6、IL-8炎症因子的表达（P<0.05）,且辣椒素与温度对IL-8的表达具有协同作用。温度升高时,辣椒素对IL-8的表达作用增强。结论 55 ℃生理盐水和辣椒素溶液能对大鼠口腔黏膜产生刺激,可引起口腔黏膜组织固有层炎细胞浸润,促进口腔黏膜上皮内IL-6和IL-8炎症因子的表达。辣椒素对口腔黏膜组织中IL-8的表达影响随温度升高而增强。

关键词: 口腔黏膜病, 辣椒素, 温度刺激, 炎症反应, 大鼠

Abstract: PURPOSE: This study investigated the effects of different temperatures and capsaicin solution on changes of morphology and inflammatory factor expressions in the oral mucosa. METHODS: The oral mucosa of rats was stimulated with normal saline (NS) and capsaicin solution at 25, 45, and 55 ℃ respectively for 4 weeks, and then the rats were sacrificed with chloral hydrate. H-E staining and immunohistochemical staining of the oral mucosa were prepared. The morphological changes of oral mucosa epithelium were observed and the expressions of TNF-α, IL-6 and IL-8 were detected. SPSS 22.0 software package was used for statistical analysis, and Graphpad Prism 8.0 software was used for statistical graphing. RESULTS: When stimulated with NS and capsaicin solution at different temperatures, the results of H-E staining showed that there was no distinct injury in the mucosal epithelium at 25 ℃ and 45 ℃. Histopathological changes were observed in the oral mucosa at 55 ℃. The expressions of IL-6 and IL-8 in the epithelium were significantly increased (P< 0.05). CONCLUSIONS: 55 ℃ NS solution and 55 ℃ capsaicin solution stimulated oral mucosa of the rats and caused infiltration of inflammatory cells in the lamina propria of the oral mucosa. They also stimulated the oral mucosa of rats, resulting in a significant increase in the expression of IL-6 and IL-8 in the oral mucosal epithelium. The effect of capsaicin on IL-8 expression was enhanced with increasing temperature.

Key words: Oral mucosa disease, Capsaicin, Thermal stimulation, Inflammatory reaction, Rat

中图分类号:

R781.5

杨一凡, 骆春梅, 李小兵, 杜芹. 温度与辣椒素对大鼠口腔黏膜的刺激效应[J]. 上海口腔医学, 2023, 32(4): 363-368.

YANG Yi-fan, LUO Chun-mei, LI Xiao-bing, DU Qin. Experimental study on the stimulation effect of temperature and capsaicin on oral mucosa of rats[J]. Shanghai Journal of Stomatology, 2023, 32(4): 363-368.

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温度与辣椒素对大鼠口腔黏膜的刺激效应

Experimental study on the stimulation effect of temperature and capsaicin on oral mucosa of rats

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