富氢水减轻丝线诱导的SD大鼠妊娠牙龈炎

doi:10.19439/j.sjos.2018.03.007

上海口腔医学 ›› 2018, Vol. 27 ›› Issue (3): 252-256.doi: 10.19439/j.sjos.2018.03.007

富氢水减轻丝线诱导的SD大鼠妊娠牙龈炎

石运芝¹，靳松²，郑慧慧¹，秦汉¹，宋国华³，秦树存³

1.泰山医学院口腔医学院，山东泰安 271000；
2.泰安市中心医院口腔科，山东泰安 271099；
3.泰山医学院动脉粥样硬化研究所, 山东泰安 271000

收稿日期:2017-06-02 修回日期:2017-09-21 出版日期:2018-07-20 发布日期:2018-07-20
通讯作者: 秦树存，E-mail: 13583815481@163.com
作者简介:石运芝（1973-），女，博士，副教授，E-mail: yzshi@tsmc.edu.cn
基金资助:
国家自然科学基金（81670422）；山东省泰山学者工程专项基金(ts201511057，20151110）；山东省医药卫生科技发展计划项目(2015WS0108)；泰山医学院高层次课题培养计划项目（2016GCC12）

Hydrogen rich water attenuates pregnancy gingivitis induced by ligation in SD rats

SHI Yun-zhi¹, JIN Song², ZHENG Hui-hui¹, QIN Han¹, SONG Guo-hua³, QIN Shu-cun³.

1.School of Stomatology, Taishan Medical University. Taian 271000；
2.Department of Stomatology, Taian City Central Hospital. Taian 271099；
3.Institute of Atherosclerosis, Taishan Medical University. Taian 271000, Shandong Province,China

Received:2017-06-02 Revised:2017-09-21 Online:2018-07-20 Published:2018-07-20

摘要/Abstract

摘要： 目的： 探讨氢气对牙龈炎孕鼠牙龈局部损伤的保护作用及其可能的机制。方法： 取SD雌鼠，采用结扎法诱导牙龈炎后，与雄鼠合笼交配。将孕鼠随机分为对照组、造模组和氢水组。对照组和造模组孕鼠饮用纯水，氢水组给予饱和氢水，1天2次。于妊娠第16天处死孕鼠，ELISA法检测各实验组孕鼠牙龈中Prog、SOD、TNF-α的水平，免疫组织化学和Western 免疫印迹法检测牙龈中PR、NFκB和TNF-α的表达。采用SPSS13.0软件包对数据进行统计学分析。结果： 丝线结扎可诱导SD大鼠妊娠期牙龈炎，PR主要分布于各实验组牙龈上皮细胞的胞质中，孕激素（Prog）及其受体（PR）的水平在各实验组孕鼠牙龈局部无显著差异（P>0.05）。造模组大鼠牙龈内SOD含量显著降低，NFκB和TNF-α表达显著升高。与造模组相比，氢水组孕鼠牙龈炎症反应明显减轻，牙龈内NFκB和TNF-α的表达下调。结论： 孕激素及其受体可能在实验大鼠妊娠牙龈炎的发生与发展过程中发挥间接作用，氢气可能通过抑制氧化应激相关炎症反应，减轻实验大鼠的妊娠牙龈炎。

关键词: 妊娠牙龈炎, 富氢水, SD大鼠, 氧化应激

Abstract: PURPOSE: To investigate the effect of hydrogen rich water on experimental gingivitis in SD rats during pregnancy. METHODS: Female SD rats mated with male ones were chosen to induce experimental gingivitis after ligation for 2 weeks. The pregnant rats were randomly divided into control group, model group and HW group. In the control and model group, rats were given pure water, while animals in the HW group were given hydrogen-rich water twice a day. All pregnant animals were sacrificed on day 16 of pregnancy. The level of Prog, SOD and TNF-α in the gingiva of different groups were measured by ELISA, the expression of PR, NFκB and TNF-α were determined by immunohistochemistry and Western blot. SPSS 13.0 software package was used for data analysis. RESULTS: Pregnancy gingivitis of SD rats could be induced by thread ligation. PR was mainly distributed in the gingival epithelium, while there was no significant difference of Prog and PR in the gingiva among different groups(P>0.05). Furthermore, in the model group, lower SOD level as well as higher NFκB and TNF-α level were found in the gingiva. Compared with the model group, the inflammatory response of pregnancy gingivitis in HW group was significantly suppressed along with decreased NFκB and TNF-α. CONCLUSIONS: Progesterone and its receptor may play an indirect role in the process of pregnancy gingivitis of rats. Hydrogen rich water may be beneficial in suppressing pregnancy gingivitis progress by decreasing inflammatory response related to gingival oxidative stress.

Key words: Pregnancy gingivitis, Hydrogen rich water, SD rats, Oxidative stress

中图分类号:

R781.4

石运芝，靳松，郑慧慧，秦汉，宋国华，秦树存. 富氢水减轻丝线诱导的SD大鼠妊娠牙龈炎[J]. 上海口腔医学, 2018, 27(3): 252-256.

SHI Yun-zhi, JIN Song, ZHENG Hui-hui, QIN Han, SONG Guo-hua, QIN Shu-cun.. Hydrogen rich water attenuates pregnancy gingivitis induced by ligation in SD rats[J]. Shanghai Journal of Stomatology, 2018, 27(3): 252-256.

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富氢水减轻丝线诱导的SD大鼠妊娠牙龈炎

Hydrogen rich water attenuates pregnancy gingivitis induced by ligation in SD rats

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