低幅高频振动对比格犬种植体周围骨愈合和种植体骨结合的影响

doi:10.19439/j.sjos.2021.01.004

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (1): 17-22.doi: 10.19439/j.sjos.2021.01.004

低幅高频振动对比格犬种植体周围骨愈合和种植体骨结合的影响

汪淑华^*, 孙哲^*, 厉辉, 叶鑫健, 郑胜, 冯剑颖

浙江中医药大学口腔医学院·附属口腔医院,浙江杭州 310053

收稿日期:2019-10-28 修回日期:2020-01-10 出版日期:2021-02-25 发布日期:2021-04-02
通讯作者: 冯剑颖,E-mail: twohorsejy@163.com
作者简介:汪淑华（1989-）,女,博士,讲师,E-mail：wangshuhua101@163.com; 孙哲（1995-）,男,在读硕士研究生,住院医师,E-mail: 752065843@qq.com。*并列第一作者
基金资助:
国家自然科学基金（8117079）; 浙江省自然科学基金（LQ21H270001）

The effect of low-magnitude high-frequency loading on peri-implant bone healing and implant osseointegration in Beagle dogs

WANG Shu-hua, SUN Zhe, LI Hui, YE Xin-jian, ZHENG Sheng, FENG Jian-ying

School of Stomatology, Zhejiang Chinese Medical University. Hangzhou 310053, Zhejiang Province, China

Received:2019-10-28 Revised:2020-01-10 Online:2021-02-25 Published:2021-04-02

摘要/Abstract

摘要： 目的:低幅高频（low-magnitude high-frequency,LMHF）振动已被证实在骨愈合中起到重要作用。本研究旨在评估LMHF振动直接加载于纯钛种植体后,对种植体周骨愈合及种植体骨结合的影响。方法:6只拔除下颌前磨牙及磨牙的雄性比格犬常规饲养3个月后,在双侧下颌骨前磨牙及磨牙区分别植入3颗纯钛种植体（Aadva Standard Implant Narrow,3.3 mm×8 mm）。每只比格犬随机选择一侧每天加载LMHF振动（实验组）,另一侧不进行干预（对照组）。使用特制的夹具和加载装置,将LMHF振动（8 μm、100 Hz）直接加载于种植体基台上,每周测试种植体稳定系数（ISQ值）。2周后,随机选择3只比格犬实施安乐死,8周后对剩余3只比格犬实施安乐死。组织标本固定及染色后,进行显微计算机体层扫描（micro-CT）和组织形态学分析。采用 SPSS 13.0 软件包对数据进行统计学分析。结果:实验组在加载2周LMHF振动后,与对照组相比,感兴趣区域2（距离种植体100~500 μm周围区域）组织周围成骨量显著增加（P<0.05）;8周后2组之间无显著差异（P>0.05）。在研究期间,2组间ISQ值和显微CT结果差异均无统计学意义（P>0.05）。结论:种植体早期愈合期间,低幅高频振动对种植体周围骨愈合和骨结合有促进作用。

关键词: 低幅高频振动, 种植体骨结合, 比格犬

Abstract: PURPOSE: Low-magnitude, high-frequency（LMHF） loading has been confirmed to play an important role in bone healing. The present study aimed to evaluate the effect of LMHF loading applied directly to titanium dental implants on peri-implant bone healing and implant. METHODS: The mandibular premolars and molars were extracted from six male Beagle dogs. Three months post-extraction, six titanium implants (Aadva Standard Implant Narrow, 3.3 mm×8 mm) were inserted into the mandibular premolar and molar area (three implants per side) in each of the six dogs. In each animal, one side was randomly selected to undergo daily LMHF loading (experimental group), while the other side had no further intervention (control group). The loading was applied directly to the implant abutment using an individual jig and a custom-made loading device (8 μm,100 Hz). The implant stability quotient (ISQ) was tested every week. Three dogs were euthanized after 2 weeks, and three were euthanized after 8 weeks. Tissue samples were fixed and stained for micro-computed tomography(micro-CT) and histomorphometric analyses. The data were statistically analyzed using SPSS 13.0 software package. RESULTS: The experimental group had significantly increased peri-implant bone volume relative to tissue volume in region of interest 2 (100-500 μm) compared with the control group after 2 weeks of loading (P<0.05); however, there was no significant difference between the two groups after 8 weeks (P>0.05). The ISQ value and the micro-CT results did not differ between the two groups during the study period. CONCLUSIONS: LMHF loading positively influences peri-implant bone healing and osseointegration in the early healing period.

Key words: Low-magnitude high-frequency loading, Implant osseointegration, Beagle dog

中图分类号:

R782.1

汪淑华, 孙哲, 厉辉, 叶鑫健, 郑胜, 冯剑颖. 低幅高频振动对比格犬种植体周围骨愈合和种植体骨结合的影响[J]. 上海口腔医学, 2021, 30(1): 17-22.

WANG Shu-hua, SUN Zhe, LI Hui, YE Xin-jian, ZHENG Sheng, FENG Jian-ying. The effect of low-magnitude high-frequency loading on peri-implant bone healing and implant osseointegration in Beagle dogs[J]. Shanghai Journal of Stomatology, 2021, 30(1): 17-22.

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低幅高频振动对比格犬种植体周围骨愈合和种植体骨结合的影响

The effect of low-magnitude high-frequency loading on peri-implant bone healing and implant osseointegration in Beagle dogs

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