异普黄酮对大鼠正畸牙复发过程中牙周组织改建的影响

doi:10.19439/j.sjos.2021.01.005

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (1): 23-27.doi: 10.19439/j.sjos.2021.01.005

异普黄酮对大鼠正畸牙复发过程中牙周组织改建的影响

代书林^1,2,3, 王旭霞^2,4, 乜福娇^1,2, 崔群^1,2, 韩媛媛^1,2, 张君^1,2

1.山东大学齐鲁医学院口腔医学院·口腔医院口腔正畸科,山东济南 250012;
2.山东省口腔组织再生重点实验室,山东省口腔生物材料与组织再生工程实验室,山东济南 250012;
3.冠县中心医院口腔科,山东冠县 252500;
4.山东大学齐鲁医学院口腔医学院·口腔医院口腔颌面外科,山东济南 250012

收稿日期:2019-11-25 修回日期:2020-01-09 出版日期:2021-02-25 发布日期:2021-04-02
通讯作者: 张君,E-mail:zhangj@sdu.edu.cn
作者简介:代书林（1987-）,男,在读硕士研究生,主治医师,E-mail：daishulin007@126.com
基金资助:
山东省自然科学基金（ZR2019MH113）

Effect of ipriflavone on reconstruction of periodontal tissues during recurrence of orthodontic teeth in rats

DAI Shu-lin^1,2,3, WANG Xu-xia^2,4, NIE Fu-jiao^1,2, CUI Qun^1,2, HAN Yuan-yuan^1,2, ZHANG Jun^1,2

1. Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University. Jinan 250012;
2. Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration. Jinan 250012;
3. Department of Stomatology, Guanxian Central Hospital. Guanxian 252500;
4. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University. Jinan 250012, Shandong Province, China

Received:2019-11-25 Revised:2020-01-09 Online:2021-02-25 Published:2021-04-02

摘要/Abstract

摘要： 目的:探讨异普黄酮对大鼠正畸牙移动复发过程中牙周组织改建的影响。方法:选择8周龄雄性SD大鼠24只,随机分为2组,即异普黄酮组(IP组)和对照组,每组12只。首先建立大鼠正畸牙移动后复发模型,连续加力10 d后去除加力装置。加力装置移除后,IP组给予10 mg/(kg·d)异普黄酮灌胃,对照组给予等量生理盐水灌胃。给药后0、1、3、7、10 d,局麻下制备2组大鼠上颌印模,灌注石膏模型,测量、评估复发距离。药物灌注10 d后,收集组织标本,H-E染色观察牙周组织改建,免疫组织化学染色观察骨形态发生蛋白2（BMP-2）的表达。利用Image-Pro 6.0软件分析染色切片吸光度值,采用SPSS 22.0软件包对数据进行统计学分析。结果:拆除矫治器后10 d内,2组正畸牙移动均有明显复发,IP组的复发距离显著小于对照组,第10 天时仍显著小于对照组。H-E染色和免疫组织化学染色结果显示,与体内对照组相比,IP组的牙周组织中有更多的新骨形成和更多的BMP-2表达。结论:在正畸牙移动复发过程中,异普黄酮能促进牙周组织中BMP-2的表达,促进牙周组织骨改建,有效降低正畸牙移动复发速率。

关键词: 异普黄酮, 正畸治疗, 复发, BMP-2

Abstract: PURPOSE: To investigate the effect of ipriflavone on reconstruction of periodontal tissues during recurrence of orthodontic teeth. METHODS: Twenty-four male SD rats were randomized into 2 groups, ipriflavone group(IP group) and control group, there were 12 rats in each group. The model of recurrence after movement of orthodontic teeth in rats was established. After continuous loading for 10 days, the loading devices were removed. Rats in ipriflavone group (IP group) were given ipriflavone intragastrically for 10 mg/(kg·d) after the devices were removed, while rats in the control group were given an equivalent amount of normal saline after the devices were removed. On the 0th, 1st, 3rd, 7th, and 10th day of administration, the rat maxillary impression and plaster model of two groups were prepared under local anesthesia, the distance between maxillary first molar lingual sulcus point and third molar in lingual groove point was measured to evaluate the relapse distance. After drug infusion for 10 days, the collected tissue specimens were stained with H-E to observe periodontal reconstruction, and expression of bone morphogenetic protein-2(BMP-2) was detected by immunohistochemical staining. Software Image-Pro 6.0 was used to analyze the optical density values of the stained sections. The data were analyzed with SPSS 22.0 software package. RESULTS: After removing the orthopaedic devices for 10 days , there was a significant recurrence of the movement of the orthodontic teeth in both groups. The recurrent distance of IP group was significantly smaller than that of the control group, and still significantly smaller than that of the control group at 10 d. H-E staining and immunohistochemical staining results showed that the IP group had more new bone formation and more BMP-2 expression in the periodontal tissues compared to the control group in vivo. CONCLUSIONS: In the process of recurrence of orthodontic tooth movement, ipriflavone can promote the expression of BMP-2 in periodontal tissue, improve bone remodeling of periodontal tissue, and effectively reduce the recurrent rate of orthodontic tooth movement.

Key words: Ipriflavone, Orthodontic treatment, Recurrence, BMP-2

中图分类号:

783.5

代书林, 王旭霞, 乜福娇, 崔群, 韩媛媛, 张君. 异普黄酮对大鼠正畸牙复发过程中牙周组织改建的影响[J]. 上海口腔医学, 2021, 30(1): 23-27.

DAI Shu-lin, WANG Xu-xia, NIE Fu-jiao, CUI Qun, HAN Yuan-yuan, ZHANG Jun. Effect of ipriflavone on reconstruction of periodontal tissues during recurrence of orthodontic teeth in rats[J]. Shanghai Journal of Stomatology, 2021, 30(1): 23-27.

参考文献

[1] Arliss RM, Biermann CA.Do soy isoflavones lower cholesterol, inhibit atherosclerosis, and play a role in cancer prevention?[J]. Holist Nurs Pract, 16(5): 40-48.
[2] Kruger MC, Wolber FM.Osteoporosis: modern paradigms for last century's bones[J]. Nutrients, 2016, 8(6): 376
[3] Guiglia R, Fede OD, Lo Russo L, et al.Osteoporosis, jawbones and periodontal disease[J]. Med Oral Patol Oral Cir Bucal, 2013, 18(1): e93-e99.
[4] Jagelaviciene E, Vaitkeviciene I, Šilingaite D, et al.The relationship between vitamin D and periodontal pathology[J]. Medicina (Kaunas), 2018, 54(3): E45.
[5] E LL, Xu WH, Feng L, et al. Estrogen enhances the bone regeneration potential of periodontal ligament stem cells derived from osteoporotic rats and seeded on nano-hydroxyapatite/collagen/poly(L-lactide)[J]. Int J Mol Med, 2016, 37(6): 1475-1486.
[6] Hughes FJ, Collyer J, Stanfield M, et al.The effects of bone morphogenetic protein-2, -4 , and -6 on differentiation of rat osteoblast cells in vitro[J]. Endocrinology, 1995, 136(6): 2671-2677.
[7] Zhou N, Li Q, Lin X, et al.BMP-2 induces chondrogenic differentiation, osteogenic differentiation and endochondral ossification in stem cells[J]. Cell Tissue Res, 2016, 366(1): 101-111.
[8] Koh JT, Zhao Z, Wang Z, et al.Combinatorial gene therapy with BMP2/7 enhances cranial bone regeneration[J]. J Dent Res, 2008, 87(9): 845-849.
[9] King GN, King N, Cruchley AT, et al.Recombinant human bone morphogenetic protein-2 promotes wound healing in rat periodontal fenestration defects[J]. J Dent Res, 1997, 76(8): 1460-1470.
[10] Gao X, Usas A, Tang Y, et al.A comparison of bone regeneration with human mesenchymal stem cells and muscle-derived stem cells and the critical role of BMP[J]. Biomaterials, 2014, 35(25): 6859-6870.
[11] Kim SJ, Kang Y, Park J, et al.Effects of low-intensity laser therapy on periodontal tissue remodeling during relapse and retention of orthodontically moved teeth[J]. Lasers Med Sci, 28(1): 325-333.
[12] Kim TW, Yoshida Y, Yokoya K, et al.An ultrastructural study of the effects of bisphosphonate administration on osteoclastic bone resorption during relapse of experimentally moved rat molars[J]. Am J Orthod Dentofacial Orthop, 1999, 115(6): 645-653.
[13] AlSwafeeri H, ElKenany W, Mowafy M, et al. Effect of local administration of simvastatin on postorthodontic relapse in a rabbit model[J]. Am J Orthod Dentofacial Orthop,153(6): 861-871.
[14] Vieira GM, Chaves SB, Ferreira VM, et al.The effect of simvastatin on relapse of tooth movement and bone mineral density in rats measured by a new method using micro tomography[J]. Acta Cir Bras, 30(5): 319-327.
[15] Hassan AH, Al-Hubail A, Al-Fraidi AA.Bone inductive proteins to enhance postorthodontic stability[J]. Angle Orthod, 80(6): 1051-1060.
[16] Fill TS, Carey JP, Toogood RW, et al.Experimentally determined mechanical properties of, and models for, the periodontal ligament: critical review of current literature[J]. J Dent Biomech, 2011: 312980.
[17] Ashizawa Y, Sahara N.Quantitative evaluation of newly formed bone in the alveolar wall surrounding the root during the initial stage of experimental tooth movement in the rat[J]. Arch Oral Biol, 1998, 43(6): 473-484.
[18] Li Y, Xing X, Wang H, et al.Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone[J]. Acta Pharmacol Sin, 2012, 33(1): 66-74.
[19] Liao MH, Tai YT, Cherng YG, et al.Genistein induces oestrogen receptor-α gene expression in osteoblasts through the activation of mitogen-activated protein kinases/NF-κB/activator protein-1 and promotes cell mineralisation[J]. Br J Nutr,111(1): 55-63.
[20] Ming LG, Chen KM, Xian CJ.Functions and action mechanisms of flavonoids genistein and icariin in regulating bone remodeling[J]. J Cell Physiol, 2013, 228(3): 513-521.
[21] Wang PP, Zhu XF, Yang L, et al.Puerarin stimulates osteoblasts differentiation and bone formation through estrogen receptor, p38 MAPK, and Wnt/β-catenin pathways[J]. J Asian Nat Prod Res, 2012, 14(9): 897-905.
[22] Hsu YL, Kuo PL.Diosmetin induces human osteoblastic differentiation through the protein kinase C/p38 and extracellular signal-regulated kinase 1/2 pathway[J]. J Bone Miner Res, 2008, 23(6): 949-960.
[23] Zeng W, Yan Y, Zhang F, et al.Chrysin promotes osteogenic differentiation via ERK/MAPK activation[J]. Protein Cell, 2013, 4(7): 539-547.
[24] Wang C, Meng MX, Tang XL, et al.The proliferation, differentiation, and mineralization effects of puerarin on osteoblasts in vitro[J]. Chin J Nat Med, 2014, 12(6): 436-442.
[25] Ying X, Sun L, Chen X, et al.Silibinin promotes osteoblast differentiation of human bone marrow stromal cells via bone morphogenetic protein signaling[J]. Eur J Pharmacol, 2013, 721(1-3): 225-230.
[26] Sheu SY, Tsai CC, Sun JS, et al.Stimulatory effect of puerarin on bone formation through co-activation of nitric oxide and bone morphogenetic protein-2/mitogen-activated protein kinases pathways in mice[J]. Chin Med J (Engl), 2012, 125(20): 3646-3653.

异普黄酮对大鼠正畸牙复发过程中牙周组织改建的影响

Effect of ipriflavone on reconstruction of periodontal tissues during recurrence of orthodontic teeth in rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	宫汝娟, 何磊. 正畸联合骨水平种植体修复在牙列缺损中的应用效果评价[J]. 上海口腔医学, 2024, 33(1): 76-79.
[2]	裴婧, 张林, 贾云香, 陈蔚华. BRAF V600E在成釉细胞瘤、成釉细胞癌和囊肿中的表达[J]. 上海口腔医学, 2023, 32(6): 630-634.
[3]	沈刚. 口腔正畸理论更新与技术研发的几点思考[J]. 上海口腔医学, 2023, 32(2): 113-119.
[4]	鞠雅琼, 徐晓明, 韩祥永, 马茵, 王桂香. 口腔专科护理模式对青少年正畸患者牙周健康的影响[J]. 上海口腔医学, 2022, 31(6): 657-660.
[5]	林怡君, 鄢洁雅, 王天鸽, 周志捷, 廖盛轩, 毛丽霞, 刘加强. 减数正畸治疗中中切牙牙根及牙槽骨形态变化的锥形束CT测量分析[J]. 上海口腔医学, 2022, 31(2): 211-216.
[6]	罗号, 袁壮, 吴开柳, 贺捷, 孟箭. 92例上颌骨成釉细胞瘤不同手术方式的效果评价[J]. 上海口腔医学, 2022, 31(1): 71-74.
[7]	刘帆, 刘琳, 王艳红. Insignia固定矫治器定制系统在正畸治疗中的效果评价[J]. 上海口腔医学, 2022, 31(1): 96-99.
[8]	徐万林, 朱云, 卢浩, 丁继平, 姚原, 刘胜文, 张陈平, 杨雯君. 腮腺区广泛型复发性多形性腺瘤的面神经处理及术后放疗经验：附10例报告[J]. 上海口腔医学, 2021, 30(6): 667-672.
[9]	刘明瑾, 李乐, 陈菊芳, 郝鑫, 杨儒, 田玉楼. 舌刺联合头帽颏兜与前方牵引矫治前牙反的疗效比较[J]. 上海口腔医学, 2021, 30(4): 429-434.
[10]	程洁, 张栋, 谢丽丽, 王璞, 李娟, 郝玮. 双颌前突患者正畸治疗后颞下颌关节紊乱病发生状况及危险因素分析[J]. 上海口腔医学, 2021, 30(4): 439-443.
[11]	葛伶伶, 吕倩, 白雪, 吴邵鸿, 姜喜玲. 青少年与成年人正畸治疗后上颌切牙牙根吸收程度比较[J]. 上海口腔医学, 2021, 30(2): 187-190.
[12]	黄丹妮, 陈文鑫, 熊浩峰, 胡鑫, 毛婷, 苏彤. 口腔鳞状细胞癌患者术后服用二甲双胍对预后的影响[J]. 上海口腔医学, 2021, 30(1): 61-65.
[13]	林嘉旭, 张汝弘, 张健娜, 巫晓平. 不同正畸治疗措施对错畸形患者龈沟液趋化因子CX3CL1、RANKL/OPG水平的影响[J]. 上海口腔医学, 2021, 30(1): 85-88.
[14]	李琴, 赵旭春, 王明朗, 李媛媛, 彭怡. 不同矫治技术对牙周炎患者上切牙区牙槽骨改变的影响[J]. 上海口腔医学, 2020, 29(6): 651-655.
[15]	韩爽, 赵军伟, 笪海芹, 夏平, 钱碧璇. 数字化隐形矫正技术治疗33例成人牙周病伴错畸形患者的效果评价[J]. 上海口腔医学, 2020, 29(4): 386-389.