低氧诱导因子1α在小鼠下颌第一磨牙萌出过程中的表达及意义

doi:10.19439/j.sjos.2021.01.003

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (1): 13-16.doi: 10.19439/j.sjos.2021.01.003

低氧诱导因子1α在小鼠下颌第一磨牙萌出过程中的表达及意义

胡秀, 康非吾

同济大学口腔医学院,同济大学附属口腔医院口腔颌面外科, 上海牙组织修复与再生工程技术研究中心,上海 200072

收稿日期:2019-10-18 修回日期:2020-01-04 出版日期:2021-02-25 发布日期:2021-04-02
通讯作者: 康非吾,E-mail：kfw@tongji.edu.cn
作者简介:胡秀（1992-）,女,硕士研究生,E-mail：1650565288@qq.com
基金资助:
国家自然科学基金（81670961）; 上海市科学技术委员会科研资助项目（医学处,医学引导类,16411961100）

Expression of hypoxia inducible factor-1alpha during mouse mandibular first molar eruption and its significance

HU Xiu, KANG Fei-wu

Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration. Shanghai 200072, China

Received:2019-10-18 Revised:2020-01-04 Online:2021-02-25 Published:2021-04-02

摘要/Abstract

摘要： 目的:研究小鼠下颌第一磨牙萌出过程中低氧诱导因子1α（hypoxia inducible factor-1alpha,HIF-1α）的表达,并探讨其表达与牙萌出及破骨细胞活性之间的关系。方法:分离出生后1.5~14.5 d小鼠的下颌骨,连续切片,对每组切片进行苏木精-伊红（H-E）染色、抗酒石酸酸性磷酸酶染色以及免疫组织化学染色。采用SPSS 25.0软件包进行统计学分析。结果:小鼠下颌第一磨牙萌出过程中,其冠方牙槽骨厚度逐渐减小,破骨细胞数量逐渐减少,HIF-1α在萌出过程中的表达逐渐减少。结论:小鼠下颌第一磨牙萌出过程中,HIF-1α表达越少,破骨细胞数量也越少。HIF-1α可能通过影响破骨细胞活性而参与小鼠下颌第一磨牙的萌出。

关键词: 低氧诱导因子1α, 牙萌出, 破骨细胞

Abstract: PURPOSE: To investigate the expression of hypoxia inducible factor-1alpha (HIF-1α) during the eruption of mouse lower first molar, and to explore the correlation between its expression and the activity of osteoclasts. METHODS: Mouse mandibles were dissected from mice at day 1.5 to 14.5 after birth. They were then serially sectioned and stained respectively using hemotoxylin, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemical staining. SPSS 25.0 software package was used for data analysis. RESULTS: During the eruption of mouse lower first molar, the thickness of the crown alveolar bone gradually decreased, so did the number of osteoclasts and the expression of HIF-1α. CONCLUSIONS: During the eruption of mouse lower first molar, the less expression of HIF-1α, the fewer osteoclasts there were. HIF-1α may be involved in the eruption of mouse lower first molar by regulating the activity of osteoclasts.

Key words: Hypoxia inducible factor-1 alpha, Tooth eruption, Osteoclasts

中图分类号:

R782.1

胡秀, 康非吾. 低氧诱导因子1α在小鼠下颌第一磨牙萌出过程中的表达及意义[J]. 上海口腔医学, 2021, 30(1): 13-16.

HU Xiu, KANG Fei-wu. Expression of hypoxia inducible factor-1alpha during mouse mandibular first molar eruption and its significance[J]. Shanghai Journal of Stomatology, 2021, 30(1): 13-16.

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低氧诱导因子1α在小鼠下颌第一磨牙萌出过程中的表达及意义

Expression of hypoxia inducible factor-1alpha during mouse mandibular first molar eruption and its significance

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