钛表面PDMS-CHXG抗菌涂层的生物相容性评价

doi:10.19438/j.cjoms.2026.03.003

摘要/Abstract

摘要： 目的: 评价纯钛表面聚二甲基硅氧烷（polydimethylsiloxane,PDMS）-氯己定（chlorhexidine digluconate,CHXG）涂层的生物相容性。方法: 将不同浓度CHXG接枝于经PDMS处理的钛片表面,将钛片随机分为Ti组（对照组）、P组（PDMS组）、C1组（0.4 mg/mL CHXG组）、C2组（0.8 mg/mL CHXG组）和C3组（1.6 mg/mL CHXG组）。采用扫描电镜观察表面形貌,能量分散型X射线能谱仪分析表面元素。体外实验采用细胞骨架染色、活/死细胞染色及CCK-8实验,系统评估体外培养牙龈上皮细胞的存活、黏附及增殖能力;将各组样品植入SD大鼠背部皮下进行体内实验,通过苏木精-伊红（HE）染色观察钛片周围的局部炎症反应。结果: 成功制备 PDMS-CHXG涂层,且氯元素含量随CHXG浓度升高而增加。Ti组、P组、C1组及C2组细胞存活率和增殖活性良好,细胞形态良好。C3组细胞存活率下降、黏附不良、增殖受到抑制。体内实验结果显示,Ti组、P组、C1组与 C2组周围组织炎症轻微,囊壁厚度相比无显著差异;C3组周围组织炎症细胞浸润明显、囊壁显著增厚。结论: PDMS-CHXG涂层在CHXG浓度≤0.8 mg/mL时具备良好的生物相容性。

关键词: 聚二甲基硅氧烷, 氯己定, 涂层, 人源牙龈上皮细胞, 背部皮下模型, 生物相容性

Abstract: PURPOSE: To evaluate the biocompatibility of polydimethylsiloxane-chlorhexidine (PDMS-CHXG) coatings constructed on titanium surfaces. METHODS: Titanium discs were modified with a PDMS coating, onto which CHXG was grafted at concentrations of 0.4, 0.8, and 1.6 mg/mL. The samples were randomly divided into 5 groups: Ti group (the control group), P group (PDMS only), C1 group (PDMS with 0.4 mg/mL CHXG), C2 group (PDMS with 0.8 mg/mL CHXG) and C3 group (PDMS with 1.6 mg/mL CHXG). Surface characteristics were examined using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). In vitro biocompatibility was assessed using human gingival epithelial cells through cytoskeleton staining, live/dead staining, and CCK-8 assays to evaluate cell viability, adhesion, and proliferation, respectively. For in vivo evaluation, the samples were implanted subcutaneously in Sprague-Dawley rats, and the surrounding tissue response was analyzed by HE staining. RESULTS: Successful fabrication of the PDMS-CHXG coating was verified, with a positive correlation observed between chlorine content and CHXG concentration. While the Ti, P, C1 and C2 groups showed robust cell health and proliferation with normal morphology, the C3 group led to reduced viability, poor adhesion, and proliferative suppression. Accordingly, in vivo data indicated minimal inflammation and similar, thin fibrous capsules for Ti, P, C1 and C2 implants, whereas the C3 group provoked a pronounced inflammatory response with significant fibrous capsule thickening. CONCLUSIONS: The study confirms that a PDMS-CHXG coating with a CHXG concentration of ≤0.8 mg/mL exhibits excellent overall biocompatibility.

Key words: Polydimethylsiloxane, Chlorhexidine, Coating, Gingival epithelial cells, Dorsal subcutaneous model, Biocompatibility

中图分类号:

R783.1

刘少梅, 夏荣. 钛表面PDMS-CHXG抗菌涂层的生物相容性评价[J]. 上海口腔医学, 2026, 35(3): 238-244.

Liu Shaomei, Xia Rong. Biocompatibility assessment of a PDMS-CHXG antibacterial coating on titanium[J]. Shanghai Journal of Stomatology, 2026, 35(3): 238-244.

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