上海口腔医学

上海口腔医学 ›› 2026, Vol. 35 ›› Issue (3): 245-251.doi: 10.19439/j.sjos.2026.03.004

• 论著 • 上一篇    下一篇

不同类型实验性牙周炎模型对小鼠牙周组织损伤及cGAS-STING 信号通路的影响

钱雪申1, 林旭馨2, 胡伟强2, 陈姣1, 夏荣1, 贾晓凤1   

  1. 1.安徽医科大学第二附属医院 口腔科,安徽 合肥 230601;
    2.福建省口腔疾病研究重点实验室,福建省口腔生物材料工程技术研究中心,福建省高校口腔医学重点实验室,福建医科大学口腔医学院/附属口腔医院,福建 福州 350002
  • 收稿日期:2026-02-06 修回日期:2026-03-26 发布日期:2026-07-02
  • 通讯作者: 贾晓凤,E-mail：812224596@qq.com
  • 作者简介:钱雪申（1992—）,女,博士,住院医师,E-mail：xueshenkq@fy.ahmu.edu.cn
  • 基金资助:
    安徽省教育厅高校科研项目（2025AHGXZK 40049）

Effect of different types of experimental periodontitis models on periodontal tissue damage and the cGASSTING signaling pathway in mice

Qian Xueshen1, Lin Xuxin2, Hu Weiqiang2, Chen Jiao1, Xia Rong1, Jia Xiaofeng1   

  1. 1. Department of Stomatology, The Second Affiliated Hospital of Anhui Medical University. Hefei 230601, Anhui Province;
    2. Fujian Key Laboratory of Oral Diseases, Fujian Provincial Engineering Research Center of Oral Biomaterial, Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University. Fuzhou 350002, Fujian Province, China
  • Received:2026-02-06 Revised:2026-03-26 Published:2026-07-02

PDF (PC)

6

摘要： 目的: 探讨不同类型实验性牙周炎模型对小鼠牙周组织的影响及其可能的分子机制。方法: 通过丝线结扎、丝线结扎联合牙龈卟啉单胞菌（Porphyromonas gingivalis, Pg ）局部注射、丝线结扎联合局部涂布Pg构建三种不同类型小鼠实验性牙周炎模型,采用Micro-CT分析骨体积（bone volume, BV）、骨体积分数（bone volume fraction, BV/TV）、骨小梁分离度（trabecular separation, Tb.Sp）和骨密度（bone mass density, BMD）水平,利用HE染色和Masson三色染色分析牙周组织的骨量变化,通过TRAP染色检测破骨细胞数量,通过免疫组织化学染色检测TNF-α、IL-1β、cGAS和STING的表达情况,采用RT-qPCR检测cGAS-STING信号通路相关基因STING、IFN-β和TBK1的表达水平。结果: 与单纯结扎及结扎联合Pg注射组相比,丝线结扎联合局部涂布Pg组小鼠牙周组织骨丧失最为明显,破骨细胞数量最多。与健康对照组相比,丝线结扎联合局部涂布Pg组小鼠牙周组织炎症因子TNF-α、IL-1β、cGAS和STING表达水平显著升高,且cGAS-STING信号通路相关基因STING、IFN-β和TBK1表达明显增加。结论: 通过丝线结扎联合局部涂布Pg构建的小鼠实验性牙周炎模型诱导更严重的牙周骨丧失,其潜在机制可能与cGAS-STING信号通路激活有关。

关键词: 牙周炎, 动物模型, 丝线结扎, 骨丧失

Abstract: PURPOSE: To investigate the effects of different types of experimental periodontitis models on periodontal tissues in mice and explore their potential molecular mechanisms. METHODS: Three models of experimental periodontitis in mice were established: ligature alone, ligature combined with local Porphyromonas gingivalis (Pg) injection, and ligature combined with local Pg topical application. Micro-CT was used to analyze bone volume (BV), bone volume fraction (BV/TV), trabecular separation (Tb.Sp), and bone mineral density (BMD). Histological analyses, including HE and Masson's trichrome staining, were performed to assess changes in alveolar bone. TRAP staining was used to detect osteoclast numbers. Immunohistochemistry was performed to evaluate the expression of TNF-α, IL-1β, cGAS, and STING. RT-qPCR was used to measure the expression of cGAS-STING pathway-related genes, including STING, IFN-β, and TBK1. RESULTS: Compared with the ligature alone and ligature + Pg injection groups, mice in the ligature + Pg topical application group exhibited the most severe alveolar bone loss and the highest number of osteoclasts. Compared with the control group, this model showed significantly increased expression of TNF-α, IL-1β, cGAS, and STING in periodontal tissues, as well as upregulated expression of cGAS-STING pathway-related genes STING, IFN-β, and TBK1. CONCLUSIONS: The ligature combined with local Pg topical application induces more severe periodontal bone loss in mice, and its underlying mechanism may be associated with activation of the cGAS-STING signaling pathway.

Key words: Periodontitis, Animal model, Ligature, Bone loss

中图分类号: