上海口腔医学

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (5): 491-496.doi: 10.19439/j.sjos.2022.05.008

• 论著 • 上一篇    下一篇

不同浓度GO对GelMA机械性能及生物相容性的影响

鲍菁1,*, 秦尉2,*, 朱青3#, 钱文昊1#   

  1. 1.上海市徐汇区牙病防治所,上海 200032;
    2.上海交通大学附属第九人民医院 口腔修复科,上海交通大学口腔医学院,国家口腔医学中心, 国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011;
    3.上海和睦家医院,上海 200336
  • 收稿日期:2021-02-25 修回日期:2021-05-11 出版日期:2022-10-25 发布日期:2022-11-01
  • 通讯作者: 朱青,E-mail: jane_zhu1988@126.com;钱文昊,E-mail：pingyanlaoto@163.com。 #共同通信作者
  • 作者简介:鲍菁(1990-),女,硕士,主治医师,E-mail：baojing9523@163.com;秦尉(1989-),女,博士,E-mail：827946040@qq.com。*并列第一作者
  • 基金资助:
    徐汇区医学科研项目（SHXH201739）

Enhanced mechanical properties, cytocompatibility of GelMA hydrogels by incorporating graphene oxide

BAO Jing1, QIN Wei2, ZHU Qing3, QIAN Wen-hao1   

  1. 1. Shanghai Xuhui District Dental Center. Shanghai 200032;
    2. Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; National Center for Stomatology; College of Stomatology, Shanghai Jiao Tong University; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
    3. Shanghai United Family Hospital. Shanghai 200336, China
  • Received:2021-02-25 Revised:2021-05-11 Online:2022-10-25 Published:2022-11-01

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摘要： 目的: 采用光固化技术合成掺杂氧化石墨烯（graphene oxide, GO）的甲基丙烯酸化明胶（methacrylated gelatin,GelMA）3D凝胶,评价其机械强度和生物相容性。方法: 将GelMA和50、100、200 μg/mL GO混合后紫外光(ultravioletray, UV)固化,使用扫描电镜(scanning electron microscope,SEM)观察水凝胶结构,万能测试仪压力实验计算弹性模量,CCK-8及死/活细胞染色检测大鼠骨髓源性间充质干细胞（rat bone marrow-derived mesenchymal stem cells, rBMSC）的增殖活性。采用SPSS 23.0软件包对数据进行统计学分析。结果: GelMA/GO复合凝胶具有高度多孔结构。加入GO可提高GelMA的机械性能,GelMA/100 μg组及GelMA/200 μg 组弹性模量显著增强, GelMA/100 μg组吸水率增加,GelMA/200 μg组体积膨胀率减小。当GO浓度为50 μg/mL时,rBMSCs黏附与增殖能力最强（P<0.05）;当GO浓度≥100 μg/mL时,rBMSCs的活性降低。结论: 合适浓度的GO可提高GelMA的机械性能和生物相容性。鉴于优先考虑生物性,建议50 μg/mL GO/GelMA 用于骨组织工程。

关键词: 光固化水凝胶, 纳米材料, 机械性能, 生物相容性

Abstract: PURPOSE: To construct graphene oxide/methacrylated gelatin(GO/GelMA) hydrogel and to investigate its mechanical property and biocompatibility. METHODS: 50, 100, 200 μg/mL GO was added to GelMA and mixed thoroughly. The micromorphology of hydrogels was observed under scanning electron microscope(SEM). Compression text was used to assess lastic modulus, CCK-8 and live/dead cell staining was conducted to assess the viability of rat bone marrow-derived mesenchymal stem cells (rBMSCs). The data were analyzed using SPSS 23.0 software package. RESULTS: SEM showed a highly porous structure of the hydrogel. After adding GO, the elastic modulous increased (100, 200 μg/mL), swelling ratio increased(100 μg/mL) and expansion ratio decreased(200 μg/mL). The viability increased in 50 μg/mL GO and decreased in 100 and 200 μg/mL GO. CONCLUSIONS: Proper concentration of GO can increase the mechanical property and biocompatibility. 50 μg/mL GO/GelMA was suggested in bone tissue engineering given priority to biocompatibility.

Key words: Phote-cure hydrogel, Nanomaterial, Mechanical property, Biocompatibility

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