吸烟对慢性牙周炎患者龈沟液及牙龈组织中β防御素2、3的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (6): 735-738.

吸烟对慢性牙周炎患者龈沟液及牙龈组织中β防御素2、3的影响

范云¹，陈栋¹，叶新¹，余方方¹，李玮²

1.郑州大学口腔医学院牙周病科，河南郑州450052；
2.郑州人民医院颐和医院口腔科，河南郑州450052

收稿日期:2015-01-23 出版日期:2015-12-25 发布日期:2016-01-04
通讯作者: 陈栋 ,Tel:0371-87097128,E-mail:chendongfmmu@163.com E-mail:fanyunkq@163.com
作者简介:范云1986-，女，硕士，医师

The impact of smoking on human beta defensin 2,3 in gingival crevicular fluid and gingival tissue of patients with chronic periodontitis

FAN Yun¹, CHEN Dong¹, YE Xin¹, YU Fang-fang¹, LI Wei².

1.Department of Periodontics, Zhengzhou University School of Stomatology. Zhengzhou 450052;
2.Department of Stomatology, Zhengzhou People’s Hospital. Zhengzhou 450052, Henan Province, China

Received:2015-01-23 Online:2015-12-25 Published:2016-01-04

摘要/Abstract

摘要： 目的探讨吸烟对慢性牙周炎患者龈沟液（gingival crevicular fluid,GCF）及牙龈组织中β防御素(human beta def-ensin,hBD)2、3表达的影响。方法将研究对象分为吸烟慢性牙周炎组和非吸烟慢性牙周炎组。采用酶联免疫吸附法（Elisa）检测hBD2、3的浓度；反转录多聚合酶链反应(RT-PCR)检测hBD2、3mRNA的表达，并做半定量分析。相关数据采用SPSS 17．0软件包进行统计学分析。结果在GCF中，hBD2、3在吸烟组的水平表达均低于非吸烟组；hBD2、3在2组牙龈组织样本中均有mRNA表达，其中吸烟组较非吸烟组mRNA表达水平减弱。结论吸烟可使GCF及牙龈组织中hBD2、3的表达发生改变，提示吸烟可对牙周宿主免疫防御系统产生消极影响。

关键词: 吸烟, 慢性牙周炎, β防御素2, β防御素3

Abstract: PURPOSE To study the impact of smoking on human beta defensin 2,3 expression in gingival crevicular fluid and gingival tissue of patients with chronic periodontitis. METHODS The study subjects were divided into chronic periodontitis with smoking and without smoking. Enzyme linked immunosorbent assay (ELISA) was used to detect the concentration of hBD2,3. RT-PCR was used to detect the mRNA expression of hBD2,3. SPSS17.0 software package was used for statistical analysis. RESULTS In GCF, the expression level of hBD2,3 in the smoking group was significantly lower than that in the non-smoking group. The mRNA expression level of hBD2,3 in the smoking group was weakened compared with that in the non-smoking group.CONCLUSIONS Smoking can change the expression of hBD2,3 in both GCF and gingivae, which indicates that smoking may have a negative effect on the immune defense system of the periodontal host.

Key words: Smoking, Chronic periodontitis, Human beta defensin 2, Human beta defensin 3

中图分类号:

R781.4

引用本文

范云，陈栋，叶新，余方方，李玮. 吸烟对慢性牙周炎患者龈沟液及牙龈组织中β防御素2、3的影响[J]. 上海口腔医学, 2015, 24(6): 735-738.

FAN Yun, CHEN Dong, YE Xin, YU Fang-fang, LI Wei.. The impact of smoking on human beta defensin 2,3 in gingival crevicular fluid and gingival tissue of patients with chronic periodontitis[J]. Shanghai Journal of Stomatology, 2015, 24(6): 735-738.

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