Swell1基因在小鼠髁突软骨发育中的时空表达

doi:10.19439/j.sjos.2021.05.003

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (5): 462-466.doi: 10.19439/j.sjos.2021.05.003

Swell1基因在小鼠髁突软骨发育中的时空表达

陈龙¹, 代杰文^1,*, 沈国芳^1,2,*

1.上海交通大学医学院附属第九人民医院口腔颅颌面科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011;
2.上海健康医学院,上海 201318

收稿日期:2021-03-22 修回日期:2021-05-10 出版日期:2021-10-25 发布日期:2021-11-08
通讯作者: 沈国芳,E-mail:shengf@sumhs.edu.cn;代杰文,E-mail:daijiewen@163.com。^*共同通信作者
作者简介:陈龙（1994-）,男,硕士研究生,E-mail:chderek12@foxmail.com
基金资助:
国家自然科学基金（82071097）

Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage

CHEN Long¹, DAI Jie-wen¹, SHEN Guo-fang^1,2

1. Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
2. Shanghai University of Medicine & Health Sciences. Shanghai 201318, China

Received:2021-03-22 Revised:2021-05-10 Online:2021-10-25 Published:2021-11-08

摘要/Abstract

摘要： 目的: 探讨Swell1(LRRC8A)基因在小鼠髁突软骨中的时空表达模式。方法: 通过获取胚胎15.5、16.5、18.5天以及新生小鼠的髁突样本,利用H-E染色、免疫荧光染色和qRT-PCR探讨小鼠髁突显微结构及软骨发育相关基因和Swell1基因的时空表达变化。采用SPSS 25.0软件包对数据进行统计学分析。结果: Swell1基因表达于髁突发育过程中,从胚胎16.5天开始,在肥大软骨细胞层表达,之后表达逐渐增强,并在小鼠胚胎发育过程中持续表达,直至小鼠出生仍有明显表达。结论: Swell1在小鼠髁发育过程中主要在肥大软骨细胞中表达,可能参与软骨细胞肥大的调控。

关键词: 小鼠, 髁突, Swell1基因, 肥大软骨细胞

Abstract: PURPOSE: To explore the temporal and spatial expression pattern of Swell1 (LRRC8A) gene in mouse condylar cartilage. METHODS: By obtaining condyle samples of embryos at 15.5, 16.5, 18.5 days and newborn mice, H-E staining, immunofluorescence staining and qRT-PCR were used to explore the microstructure of mouse condyles and the temporal and spatial expression changes of genes related to cartilage development and Swell1 gene. Statistical analysis was performed using SPSS 25.0 software package. RESULTS: Swell1 gene expression began in the hypertrophic chondrocyte layer during the development of the condyle from embryonic day 16.5, and then gradually increased, and continued to be expressed during mouse embryonic development until the mouse was born. CONCLUSIONS: Swell1 is mainly expressed in hypertrophic chondrocytes during the development of mouse condyles, and it may be involved in the regulation of chondrocyte hypertrophy.

Key words: Mouse, Mandibular condyle, Swell1, Hypertrophic chondrocytes

中图分类号:

R782

陈龙, 代杰文, 沈国芳. Swell1基因在小鼠髁突软骨发育中的时空表达[J]. 上海口腔医学, 2021, 30(5): 462-466.

CHEN Long, DAI Jie-wen, SHEN Guo-fang. Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage[J]. Shanghai Journal of Stomatology, 2021, 30(5): 462-466.

参考文献

[1] Hirouchi H, Kitamura K, Yamamoto M, et al.Developmental characteristics of secondary cartilage in the mandibular condyle and sphenoid bone in mice[J]. Arch Oral Biol, 2018, 89(1): 84-92.
[2] Yahara Y, Takemori H, Okada M, et al.Pterosin b prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting sik3[J]. Nat Commun, 2016, 7: 10959.
[3] Sun MM, Beier F.Chondrocyte hypertrophy in skeletal development, growth, and disease[J]. Birth Defects Res C Embryo Today, 2014, 102(1): 74-82.
[4] Ji H, Li J, Shao J, et al.Histopathologic comparison of condylar hyperplasia and condylar osteochondroma by using different staining methods[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2017, 123(3): 320-329.
[5] Ripmeester EGJ, Timur UT, Caron MMJ, et al. Recent insights into the contribution of the changing hypertrophic chondrocyte phenotype in the development and progression of osteoarthritis [J]. Front Bioeng Biotechnol, 2018, 6: 18.e1-18.e25.
[6] Cahalan MD, Lewis RS.Role of potassium and chloride channels in volume regulation by T lymphocytes[J]. Soc Gen Physiol Ser, 1988, 43: 281-301.
[7] Osei-Owusu J, Yang J, Vitery MDC, et al.Molecular biology and physiology of volume-regulated anion channel (vrac)[J]. Curr Top Membr, 2018, 81: 177-203.
[8] Kumar L, Chou J, Yee CS, et al.Leucine-rich repeat containing 8a (lrrc8a) is essential for T lymphocyte development and function[J]. J Exp Med, 2014, 211(5): 929-942.
[9] Zhang Y, Xie L, Gunasekar SK, et al.Swell1 is a regulator of adipocyte size, insulin signalling and glucose homeostasis[J]. Nat Cell Biol, 2017, 19(5): 504-517.
[10] Yamada T, Strange K.Intracellular and extracellular loops of lrrc8 are essential for volume-regulated anion channel function[J]. J Gen Physiol, 2018, 150(7): 1003-1015.
[11] Qiu Z, Dubin AE, Mathur J, et al.Swell1, a plasma membrane protein, is an essential component of volume-regulated anion channel[J]. Cell, 2014, 157(2): 447-458.
[12] Hunziker EB.Mechanism of longitudinal bone growth and its regulation by growth plate chondrocytes[J]. Microsc Res Tech, 1994, 28(6): 505-519.
[13] Song H, Park KH.Regulation and function of sox9 during cartilage development and regeneration[J]. Semin Cancer Biol, 2020, 67(Pt 1): 12-23.
[14] Lu P, Ding Q, Li X, et al.Swell1 promotes cell growth and metastasis of hepatocellular carcinoma in vitro and in vivo[J]. EBioMedicine, 2019, 48: 100-116.
[15] Chen L, König B, Stauber T.Lrrc8 channel activation and reduction in cytosolic chloride concentration during early differentiation of c2c12 myoblasts[J]. Biochem Biophys Res Commun, 2020, 532(3): 482-488.
[16] Rubino S, Bach MD, Schober AL, et al. Downregulation of leucine-rich repeat-containing 8a limits proliferation and increases sensitivity of glioblastoma to temozolomide and carmustine[J]. Front Oncol, 2018, 8: 142.e1-142.e12.

Swell1基因在小鼠髁突软骨发育中的时空表达

Temporal and spatial expression of Swell1 gene in the development of mouse condyle cartilage

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘丽敏, 孙晶晶, 钱佳骏, 张春叶, 胡宇华, 李江. 171例颌面部骨软骨瘤及恶变病例临床病理分析[J]. 上海口腔医学, 2024, 33(3): 324-327.
[2]	郁莹, 翁清清, 罗银月, 姚姝冉, 易芳羽, 张颖. 过量氟对小鼠髁突软骨损伤及自噬相关因子LC3、p62表达的影响[J]. 上海口腔医学, 2024, 33(2): 113-116.
[3]	张雪冰, 李琦. PRF负载的Genistein促进肥胖小鼠骨缺损修复的实验研究[J]. 上海口腔医学, 2024, 33(1): 13-21.
[4]	应凯, 王亮, 朱永武, 李倩倩, 毛斌. 虚拟手术结合导板辅助7例髁突颈部骨折复位固定的效果评价[J]. 上海口腔医学, 2023, 32(1): 105-108.
[5]	沈刚. 含颌位因素错畸形与颌位重建基本原理[J]. 上海口腔医学, 2022, 31(6): 561-568.
[6]	张春香, 王天齐, 刘冬巧, 段小妮, 智铖, 王静, 林晨. 伴颞下颌关节紊乱病安氏Ⅱ类1分类错患者咀嚼运动中髁突轨迹分析[J]. 上海口腔医学, 2022, 31(4): 429-434.
[7]	姚志涛, 安玮, 买买提吐逊·吐尔地. 保守治疗对儿童单侧髁突骨折患者颌骨对称性的影响[J]. 上海口腔医学, 2021, 30(1): 77-80.
[8]	龚心仪, 黄湘如, 周巳入, 杨屹羚, 徐弘远, 金安婷, 代庆刚, 江凌勇. 诱导型成骨细胞特异Stat3基因敲除小鼠的构建及验证[J]. 上海口腔医学, 2020, 29(4): 337-342.
[9]	雷杰, 肖遥, 罗茂璇, 徐晓梅. 12~14岁骨性Ⅱ类高角女性拔牙矫治前、后髁突位置与上气道结构变化[J]. 上海口腔医学, 2020, 29(3): 281-286.
[10]	冷春涛, 李婷, 高晓蔚. 4种白细胞介素在种植体周围炎分期模型中的表达分析[J]. 上海口腔医学, 2020, 29(2): 162-167.
[11]	李文艳, 陈文静, 侯伟, 秦金炜, 段义峰, 穆锦全. 不同骨型人群髁突不对称性的锥形束CT评价[J]. 上海口腔医学, 2019, 28(5): 523-528.
[12]	陈霜, 钟群, 翁蓓军, 叶页, 钱玉梅. 牙列重度磨耗患者咬合重建前、后颞下颌关节的线性测量[J]. 上海口腔医学, 2019, 28(3): 275-278.
[13]	王明锋, 李爽, 刘琳. 减小后牙▉平面倾斜度对安氏Ⅱ类高角畸形髁突位置影响的研究[J]. 上海口腔医学, 2018, 27(4): 386-389.
[14]	沈刚. SGTB 矫形诱发髁突改建的生物机制及临床意义[J]. 上海口腔医学, 2018, 27(3): 225-229.
[15]	刘帅, 赵震锦, 赵瑞, 郑雪松, 王丽萍. 下颌偏斜患者髁突位置的meta分析[J]. 上海口腔医学, 2018, 27(2): 210-215.