IGF2BP1在口腔鳞癌细胞耐药中的作用及机制探讨

doi:10.19439/j.sjos.2021.05.002

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (5): 456-461.doi: 10.19439/j.sjos.2021.05.002

IGF2BP1在口腔鳞癌细胞耐药中的作用及机制探讨

谢非, 秦星, 童桐, 蒋英英, 张建军

上海交通大学医学院附属第九人民医院口腔颌面-头颈肿瘤科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011

收稿日期:2020-05-28 修回日期:2020-06-28 出版日期:2021-10-25 发布日期:2021-11-08
通讯作者: 张建军,E-mail:zjjshuobo@163.com
作者简介:谢非（1994-）,女,硕士研究生,E-mail:xiefeix@hotmail.com
基金资助:
国家自然科学基金（81972573、81772933）; 上海市科学技术委员会科研计划项目（18JC1413700）; 上海高水平地方高校创新团队

Insight into the role of IGF2BP1 in drug resistant mechanism of oral squamous cell carcinoma

XIE Fei, QIN Xing, TONG Tong, JIANG Ying-ying, ZHANG Jian-jun

Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2020-05-28 Revised:2020-06-28 Online:2021-10-25 Published:2021-11-08

摘要/Abstract

摘要： 目的: 探讨IGF2BP1在口腔鳞癌细胞顺铂耐药中的作用和机制。方法: 运用小剂量间歇诱导法诱导顺铂敏感的口腔鳞癌细胞HN30,建立顺铂耐药口腔鳞癌细胞系HN30/DDP。通过蛋白免疫印迹方法检测亲本株与耐药株的IGF2BP1表达差异;利用siRNA和慢病毒过表达载体分析IGF2BP1基因表达水平降低或升高对癌细胞顺铂耐药的影响;应用MTT法检测IGF2BP1基因降低或升高后口腔鳞癌细胞对顺铂的IC₅₀。采用SPSS 17.0软件包对数据进行统计学分析。结果: 成功建立顺铂耐药的口腔鳞癌细胞系HN30/DDP,耐药细胞株较亲本株的耐药性明显提高。敲低HN30/DDP中的 IGF2BP1 表达水平,细胞耐药性降低;反之,亲本株细胞过表达 IGF2BP1后,细胞耐药性提高。Akt 信号通路活化是介导 IGF2BP1 促进口腔鳞癌细胞顺铂耐药的关键因素。结论: IGF2BP1 与口腔鳞癌的顺铂耐药密切相关。IGF2BP1 可通过激活下游 Akt 信号通路,促进口腔鳞癌细胞耐药。

关键词: 口腔鳞癌, IGF2BP1, 化疗耐药, 顺铂

Abstract: PURPOSE: This study focused on the role and mechanism of IGF2BP1 in cell cisplatin resistance of oral squamous cell carcinoma. METHODS: Low-dose intermittent induction method was used to induce cisplatin-sensitive oral squamous cell carcinoma cell line HN30, and establish cisplatin-resistant cell line HN30/DDP. Western blot was used to detect the protein expression level of IGF2BP1 in parental and resistant cell line. Knockdown or overexpression of IGF2BP1 by RNAi and lentivirus transfection method was utilized to analyze the effect of decreased or increased the gene expression of IGF2BP1 on cisplatin resistance. MTT method was used to detect the change of IC₅₀. Statistical analysis of data was performed using SPSS 17.0 software package. RESULTS: Cisplatin-resistant oral squamous cell carcinoma cell line was successfully established. The IC₅₀ of the drug-resistant cells was significantly higher than that of the parental cells. Knocking down the expression level of IGF2BP1 in drug-resistant strains reduced cell resistance; on the contrary, after overexpression of IGF2BP1 in parental cells dramatically increased cisplatin resistance. Mechanically, activation of Akt signaling pathway was the key factor mediating IGF2BP1 to promote cisplatin resistance in oral squamous cell carcinoma. CONCLUSIONS: IGF2BP1 is significantly associated with cisplatin resistance in oral squamous cell carcinoma. IGF2BP1 can promote cisplatin resistance of oral squamous cells by activating downstream Akt signaling pathway.

Key words: Oral squamous cell carcinoma, IGF2BP1, Chemotherapy resistance, Cisplatin

中图分类号:

R739.8

谢非, 秦星, 童桐, 蒋英英, 张建军. IGF2BP1在口腔鳞癌细胞耐药中的作用及机制探讨[J]. 上海口腔医学, 2021, 30(5): 456-461.

XIE Fei, QIN Xing, TONG Tong, JIANG Ying-ying, ZHANG Jian-jun. Insight into the role of IGF2BP1 in drug resistant mechanism of oral squamous cell carcinoma[J]. Shanghai Journal of Stomatology, 2021, 30(5): 456-461.

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IGF2BP1在口腔鳞癌细胞耐药中的作用及机制探讨

Insight into the role of IGF2BP1 in drug resistant mechanism of oral squamous cell carcinoma

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