不同根管消毒方法对粪肠球菌清除作用的体外研究

doi:10.19439/j.sjos.2017.02.002

上海口腔医学 ›› 2017, Vol. 26 ›› Issue (2): 134-138.doi: 10.19439/j.sjos.2017.02.002

不同根管消毒方法对粪肠球菌清除作用的体外研究

孙楚文, 朱亚琴

上海交通大学医学院附属第九人民医院·口腔医学院口腔综合科, 上海市口腔医学重点实验室,上海 200011

收稿日期:2016-09-01 修回日期:2016-10-30 出版日期:2017-04-25 发布日期:2017-05-04
通讯作者: 朱亚琴,E-mail:zyq1590@163.com
作者简介:孙楚文（1990-）,女,硕士,住院医师,E-mail：s_icebin6002@163.com
基金资助:
国家自然科学基金（81271134）; 上海高校高峰高原学科建设项目

Elimination of Entercoccus faecalis with different disinfection methods in root canals in vitro

SUN Chu-wen, ZHU Ya-qin

Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2016-09-01 Revised:2016-10-30 Online:2017-04-25 Published:2017-05-04

摘要/Abstract

摘要： 目的: 比较被动超声荡洗（passive ultrasonic irrigation,PUI）与半导体激光,及两者联用对根管内粪肠球菌的清除作用,并与3%NaClO消毒效果进行比较,为临床选择根管消毒方法提供参考。方法: 选取50颗成人单根管离体前磨牙,建立粪肠球菌生物膜感染根管模型,随机将其均分为5组并做不同处理。A组为生理盐水冲洗组,B组为3%NaClO溶液冲洗组（阳性对照组）,C组为PUI处理组,D组为半导体激光处理组,E组为PUI联合半导体激光处理组。选用纸尖法取样,稀释培养48 h后,计算每个样本菌落形成单位（colony-forming units,CFU）值。采用SAS 8.02软件包对数据进行统计学分析。结果: PUI处理组、半导体激光处理组及联合处理组样本的CFU值,与生理盐水冲洗组相比均存在显著差异（P<0.01）,3组结果均显著低于生理盐水冲洗组,而3组之间则无显著差异（P>0.05）。3%NaClO溶液冲洗组（阳性对照组）的CFU值均显著低于其余4组（P<0.01）。结论: PUI、半导体激光消毒及其两者联用均对粪肠球菌生物膜具有显著消毒效果,显著优于单纯使用生理盐水冲洗,但三者之间效果相当;3%NaClO冲洗对粪肠球菌生物膜具有最强的消毒作用。

关键词: 根管消毒, 粪肠球菌, 次氯酸钠, 被动超声荡洗, 半导体激光

Abstract: PURPOSES: To compare the elimination effect against E.faecalis in root canals with different methods. METHODS: Fifty extracted premolars with single root canal were selected. After cleaning and autoclaving, they were contaminated by E.faecalis and incubated for 28 days as models. Then the models were randomly and equally divided into 5 groups and treated as below: specimens in group A were treated with saline irrigation, specimens in group B were treated with 3% NaClO irrigation (as positive control), specimens in group C were treated with PUI, specimens in group D were treated with diode laser radiation, specimens in group E were treated with combination of PUI and diode laser radiation. The specimens from root canals were collected by paper points. The bacterial suspensions were later serially diluted and plated on tryptic soy agar plates to enumerate the CFUs after 48 hours. Statistical analysis was performed using SAS 8.02 software package. RESULTS: As with all parts of the root canal in aggregate, the CFUs of the specimens treated with PUI, diode laser radiation and the combination of them were significantly lower than the specimens treated with saline irrigation (P<0.01), but there was no significant difference among 3 groups (P>0.05). However, the specimens treated with 3% NaClO irrigation had the best effect of disinfection. The number of CFUs in the specimens treated with 3% NaClO was almost zero. There was no significant difference between this group and others in CFUs(P<0.01). CONCLUSIONS: Specimens treated with PUI, diode laser radiation and the combination of them showed great effect of elimination against biofilm of Enterococcus faecalis compared with saline irrigation. Irrigation with 3% NaClO was the most efficient method in this experiment.

Key words: Root canal disinfection, Enterococcus faecalis, Sodium hypochlorite, PUI, Diode laser

中图分类号:

R781.3

孙楚文, 朱亚琴. 不同根管消毒方法对粪肠球菌清除作用的体外研究[J]. 上海口腔医学, 2017, 26(2): 134-138.

SUN Chu-wen, ZHU Ya-qin. Elimination of Entercoccus faecalis with different disinfection methods in root canals in vitro [J]. Shanghai Journal of Stomatology, 2017, 26(2): 134-138.

参考文献

[1] Lorencetti KT, Silva-Sousa YT, Nascimento GE, et al. Influence of apical enlargement in cleaning of curved canals using negative pressure system [J]. Braz Dent J, 2014, 25(5): 430-434.
[2] Frough Reyhani M, Rahimi S, Fathi Z, et al. Evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of Enterococcus faecalis [J]. J Dent Res Clin Dent Prospects, 2015, 9(3): 138-143.
[3] Ganesh A, Nagendrababu V, John A, et al. The effect of addition of an EPS degrading enzyme with and without detergent to 2% chlorhexidine on disruption of Enterococcus faecalis biofilm: a confocal laser scanning microscopic study [J]. J Clin Diagn Res, 2015, 9(11): ZC61-65.
[4] Kivanc BH, Arsu HD, Yanar NÖ, et al. Apical extrusion of sodium hypochlorite activated with two laser systems and ultrasonics: a spectrophotometric analysis [J]. BMC Oral Health, 2015, 15(1): 71.
[5] Wang Z, Shen Y, Haapasalo M. Effect of smear layer against disinfection protocols on Enterococcus faecalis -infected dentin [J]. J Endod, 2013, 39(11): 1395-1400.
[6] Giardino L, Estrela C, Generali L, et al. The in vitro effect of irrigants with low surface tension on Enterococcus faecalis [J]. Iran Endod J, 2015, 10(3): 174-178.
[7] Weller RN, Brady JM, Bernier WE. Efficacy of ultrasonic cleaning [J]. J Endod, 1980, 6(9): 740-743.
[8] Khaord P, Amin A, ShahMB, et al. Effectiveness of different irrigation techniques on smear layer removal in apical thirds of mesial root canals of permanent mandibular first molar: A scanning electron microscopic study[J]. J Conserv Dent, 2015, 18(4): 321-326.
[9] Mozo S, Llena C, Chieffi N, et al. Effectiveness of passive ultrasonic irrigation in improving elimination of smear layer and opening dentinal tubules [J]. J Clin Exp Dent, 2014, 6(1): 47-52.
[10] Kanumuru NR, Subbaiah R. Bacterial efficacy of Ca(OH) 2 against E.faecalis compared with three dental lasers on root canal dentin- an in vitro study [J]. J Clin Diagn Res, 2014, 8(11): ZC135-137.
[11] Gerek M, Asci S, Yaylali DI. Ex vivo evaluation of antibacterial effects of Nd:YAG and diode lasers in root canals [J]. Biotechnol Biotechnol Equip, 2014, 24(3): 2031-2034.
[12] López-Jiménez L, Arnabat-Domínguez J, Viñas M, et al. Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers [J]. Med Oral Patol Oral Cir Bucal, 2015, 20(1): e45-51.
[13] Joy J, Mathias J, Sagir VMM, et al. Bacterial biofilm removal using static and passive ultrasonic irrigation [J]. J Int Oral Health, 2015, 7(7): 42-47.
[14] Brittan J, Sprague S, Huntley S, et al. Collagen-like peptide sequences inhibit bacterial invasion of root dentine [J]. Int Endod J, 2016, 49(5): 462-470.
[15] Ran S, Gu S, Wang J, et al. Dentin tubule invasion by Enterococcus faecalis under stress conditions ex vivo [J]. Eur J Oral Sci, 2015, 123(5): 362-368.
[16] Lim Z, Cheng JL, Lim TW, et al. Light activated disinfection: an alternative endodontic disinfection strategy [J]. Aust Dent J, 2009, 54(2): 108-114.
[17] Boutsioukis C, Psimma Z, van der Sluis LW. Factors affecting irrigant extrusion during root canal irrigation: a systematic review [J]. Int Endod J, 2013, 46(7): 599-618.
[18] Guerreiro-Tanomaru JM, Chávez-Andrade GM, Faria-Júnior NB, et al. Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: an ex vivo study [J]. Braz Dent J, 2015, 26(4): 342-346.
[19] Sohrabi K, Sooratgar A, Zolfagharnasab K, et al. Antibacterial activity of diode laser and sodium hypochlorite in Enterococcus faecalis -contaminated root canals [J]. Iran Endo J, 2016, 11(1): 8-12.
[20] Alfredo E, Marchesan MA, Sousa-Neto MD, et al. Temperature variation at the external root surface during 980-nm diode laser irradiation in the root canal [J]. J Dent, 2008, 36(7): 529-534.
[21] Krause T, Liewehr F, Hann C. The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline and citric acid on Enterococcus faecalis [J]. J Endod, 2007, 33(1): 28-30.
[22] Preethee T, Kandaswamy D, Arathi G. Bactericidal effect of the 908 nm diode laser on Enterococcus faecalis in infected root canals [J]. J Conserv Dent, 2012, 15(1): 46-50.
[23] Mehrvarzfar P, Saghiri MA, Asatourian A, et al. Additive effect of a diode laser on the antibacterial activity of 2.5% NaOCl, 2% CHX and MTAD against Enterococcus faecalis contaminating root canals: an in vitro study [J]. J Oral Sci, 2011, 53(3): 355-360.

不同根管消毒方法对粪肠球菌清除作用的体外研究

Elimination of Entercoccus faecalis with different disinfection methods in root canals in vitro

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘昭辰, 孙培, 潘克清, 王培彦, 张慧, 袁昌青, 邓婧. 次氯酸溶液的稳定性及对口腔组织刺激性的体外研究[J]. 上海口腔医学, 2024, 33(2): 141-147.
[2]	方颖, 黄文燕, 苗国厚, 孟斯, 彭博, 曾素娟. 新型生物活性玻璃对乳牙根尖周炎常见细菌抗菌效果评价[J]. 上海口腔医学, 2024, 33(1): 49-53.
[3]	刘慧楠, 张富华, 蒲灼光. 不同荡洗策略治疗有瘘型慢性根尖周炎的临床效果比较[J]. 上海口腔医学, 2023, 32(2): 188-192.
[4]	单晓阳, 孙莉青, 王月月, 杨楠, 孙慧斌. 微创开髓下3种根管消毒方法对粪肠球菌清除效果比较[J]. 上海口腔医学, 2022, 31(1): 17-23.
[5]	张彬, 杨贝贝, 郜珍燕, 李莉, 安虹. 半导体激光辅助直接盖髓术在龋源性露髓患牙中的效果评价[J]. 上海口腔医学, 2020, 29(5): 554-556.
[6]	陆佳萍, 黄正蔚, 钱文昊. 根管负压冲洗对根尖推出物及根管内粪肠球菌的影响[J]. 上海口腔医学, 2019, 28(6): 601-604.
[7]	汪嘉, 赵申, 冉淑君, 孙喆, 梁景平. 不同根管冲洗方式对粪肠球菌生物膜清除效果评价[J]. 上海口腔医学, 2019, 28(3): 246-250.
[8]	郭彪, 路荣建, 葛成. 半导体激光辅助治疗78例窦道型根尖周脓肿的效果观察[J]. 上海口腔医学, 2018, 27(4): 427-429.
[9]	毕静, 刘尧, 陈旭. iRoot FM对牙髓卟啉单胞菌抗菌效果评价[J]. 上海口腔医学, 2017, 26(5): 488-491.
[10]	陆春慧, 钟群. 应用水激光和超声进行根管消毒的效果评价[J]. 上海口腔医学, 2017, 26(3): 314-316.
[11]	王昊明, 周梦琪, 洪瑾. Er:YAG激光对犬根尖周组织影响的组织学评价[J]. 上海口腔医学, 2016, 25(6): 657-662.
[12]	江美媛, 游清玲. 半导体激光理疗联合A型肉毒毒素治疗颞下颌关节紊乱病的效果评价[J]. 上海口腔医学, 2016, 25(6): 758-761.
[13]	陈晖, 张旸, 汪俊. 全脱位牙延期再植根面牙周膜不同去除方法的比较[J]. 上海口腔医学, 2016, 25(3): 296-300.
[14]	杨红丽，唐睿，龚瑜，张玉皓. 根管预备及根管消毒期间诊间疼痛的临床观察[J]. 上海口腔医学, 2016, 25(2): 227-230.
[15]	张富华，李矛，韦智君，赵兵. 纳米银颗粒联合氢氧化钙对饥饿期粪肠球菌生物膜的作用[J]. 上海口腔医学, 2016, 25(1): 11-15.