大鼠胫骨放射性骨坏死模型的建立及评估

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (5): 513-516.

• 论著 • 下一篇

大鼠胫骨放射性骨坏死模型的建立及评估

韩伟¹, 周勇², 吴轶群³, 王志勇¹, 张雷², 邹多宏², 张凯⁴, 王元银², 何家才², 张志愿³

1.南京市口腔医院·南京大学医学院附属口腔医院口腔颌面外科,江苏南京 210008；
2.安徽医科大学附属口腔医院牙种植中心,安徽省口腔疾病研究省级重点实验室,安徽合肥 230032；
3.上海交通大学医学院附属第九人民医院·口腔医学院,上海 200011；
4.蚌埠医学院第一附属医院口腔科,安徽蚌埠 233000

收稿日期:2016-01-11 修回日期:2016-05-04 出版日期:2016-10-25 发布日期:2016-11-10
通讯作者: 邹多宏,E-mail：zdhyy@ahmu.edu.cn
作者简介:韩伟(1979-),男,博士,副主任医师,E-mail: doctorhanwei@hotmail.com
基金资助:
国家自然科学基金 (31370983,81302351); 安徽省杰出青年基金 (1508085J08); 安徽省教育厅项目 (KJ2013Z203); 安徽省科技攻关计划 (1401045013, 1604a0802082); 高校优秀青年人才支持计划重点项目 (gxyqZD2016058); 江苏省自然科学基金（BK20131080）

Establishment and assessment of tibial osteoradionecrosis in rats

HAN Wei¹, ZHOU Yong², WU Yi-qun³, WANG Zhi-yong¹, ZHANG Lei², ZOU Duo-hong², ZHANG Kai⁴, WANG Yuan-yin², HE Jia-cai², ZHANG Zhi-yuan³

1.Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University. Nanjing 210008, Jiangsu Province;
2.Department of Dental Implant Center, Stomatologic Hospital & College, Anhui Medical University, Key Laboratory of Oral Diseases Research of Anhui Province. Hefei 230032, Anhui Province;
3.Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine. Shanghai 200011;
4.Department of Stomatology, the First Hospital Affiliated to Bengbu Medical College. Bengbu 233000, Anhui Province, China

Received:2016-01-11 Revised:2016-05-04 Online:2016-10-25 Published:2016-11-10

摘要/Abstract

摘要： 目的建立大鼠胫骨放射性骨坏死动物模型,通过大体观察和组织病理学方法对该模型进行评估。方法选择6只Sprague-Dawley （SD）大鼠（体重200~250 g）,使用直线加速器对 SD 大鼠右侧胫骨区进行60 Gy的照射,照射分4次完成,每次间隔2周。放疗后 1、2、3及4周,大体观察放疗区变化,评估大鼠下肢放疗后毛发及皮肤改变。放疗后4周,利用组织病理学方法比较放疗区与非放疗区骨组织的变化,通过影像学检查判断骨破坏情况。采用SPSS 13.0 软件包对数据进行统计学分析。结果SD 大鼠在完成放疗后1周,放疗区无显著变化,但与对侧相比,放疗侧下肢爪心颜色明显变红;放疗后2~3周,放疗区毛发脱落;放疗后4周,放疗区毛发完全脱落,且皮肤发生溃疡;放疗4周后组织病理切片H-E染色显示,放疗侧骨量显著少于对照侧,包括骨壁厚度、骨髓细胞及骨小梁数目。影像学检查发现放疗区明显骨破坏。结论使用60 Gy剂量对SD 大鼠胫骨分别进行4次照射,可成功构建大鼠胫骨放射性骨坏死模型。

关键词: 放射性骨坏死, 大鼠, 动物模型

Abstract: PURPOSE: To establish a rat tibial osteoradionecrosis model and assess the model by observing clinical manifestation and histopathological examinations. METHODS: The right tibia of 6 Sprague Dawley (SD) rats (weighing 200-250 g) was irradiated by a linear accelerator (single fraction 15 Gy, total dose 60 Gy) every second week for four times. Hair and cutaneous changes of the irradiated region were observed one, two, three and four weeks after irradiation, respectively. Histopathological examination was employed to compare the bone tissue between the irradiated region and irradiation-free region. X-ray film was taken to evaluate the bone destruction. SPSS13.0 software package was used for statistical analysis. RESULTS: One week after irradiation, no significant change was observed in the irradiated region. However, the palm of the irradiated limb turned red remarkably in contrast with the opposite one. Two and three weeks after irradiation, trichomadesis was observed in the irradiated region. Four weeks after irradiation, complete trichomadesis and skin ulceration occurred in the irradiated region. In histopathological examinations, hematoxylin-eosin staining showed that bone mass including bone plate thickness, bone marrow cell, bone trabecula decreased significantly in the irradiated region compared with the control side. Moreover, significant bone destruction was found in irradiated group. CONCLUSIONS: We have successfully established a rat tibial model of osteoradionecrosis with radiation at dosage of 60 Gy divided into 4 times.

Key words: Osteoradionecrosis, Rat, Animal model

中图分类号:

R782.1

韩伟, 周勇, 吴轶群, 王志勇, 张雷, 邹多宏, 张凯, 王元银, 何家才, 张志愿. 大鼠胫骨放射性骨坏死模型的建立及评估[J]. 上海口腔医学, 2016, 25(5): 513-516.

HAN Wei, ZHOU Yong, WU Yi-qun, WANG Zhi-yong, ZHANG Lei, ZOU Duo-hong, ZHANG Kai, WANG Yuan-yin, HE Jia-cai, ZHANG Zhi-yuan. Establishment and assessment of tibial osteoradionecrosis in rats[J]. Shanghai Journal of Stomatology, 2016, 25(5): 513-516.

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大鼠胫骨放射性骨坏死模型的建立及评估

Establishment and assessment of tibial osteoradionecrosis in rats

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