成纤维细胞生长因子受体家族在头颈鳞癌细胞中的表达及定位

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (5): 517-521.

成纤维细胞生长因子受体家族在头颈鳞癌细胞中的表达及定位

石超吉, 周榕, 韩永, 陶文杰, 孙树洋, 张志愿

上海交通大学医学院附属第九人民医院·口腔医学院,上海市口腔医学重点实验室,上海 200011

收稿日期:2016-01-15 修回日期:2016-03-03 出版日期:2016-10-25 发布日期:2016-11-10
通讯作者: 张志愿,E-mail：zhzhy0502@163.com
作者简介:石超吉（1988-）,男,硕士,E-mail:scjtdcq@126.com
基金资助:
国家自然科学基金(81202131)

Expression and localization of FGFR family in squamous cell carcinoma of head and neck

SHI Chao-ji, ZHOU Rong, HAN Yong, TAO Wen-jie, SUN Shu-yang, ZHANG Zhi-yuan

Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2016-01-15 Revised:2016-03-03 Online:2016-10-25 Published:2016-11-10

摘要/Abstract

摘要： 目的探讨成纤维细胞生长因子受体在头颈鳞癌细胞中的表达及其定位。方法提取10种头颈鳞癌细胞系蛋白,采用Western印迹技术检测FGFR家族成员的表达;利用免疫荧光技术检测FGFR各型在SCC25和HN6细胞中的表达位置。应用Image J软件测量免疫印迹条带灰度值,GraphPad Prism 5.01对灰度值结果进行统计学分析。结果在10株头颈鳞癌细胞细中,6株表达FGFR1。FGFR2、3、4均在10株头颈鳞癌细胞细中表达。FGFR1、2、4表达于细胞核及细胞质,FGFR3仅表达于细胞质。结论FGFR在头颈鳞癌细胞细中共表达,可能与头颈鳞癌的发生、发展有关;FGFR家族不同亚型在头颈鳞癌细胞的表达定位存在差异。

关键词: 成纤维细胞生长因子受体, 鳞癌, 头颈部

Abstract: PURPOSE: To investigate the expression and localization of FGFR family in squamous cell carcinoma of head and neck (SCCHN) cell lines. METHODS: Total protein was extracted from 10 SCCHN cell lines and the expression of FGFR was detected by Western blot. The localization of FGFR was further demonstrated by immunofluorescence staining in SCC25 and HN4 cell lines. Gray value was measured by Image J. GraphPad Prism 5.01 software package was used for data processing and analysis. RESULTS: FGFR1 expression was detected in 6/10 cell lines and FGFR2, 3, 4 was detectable in all cell lines. The expression of FGFR1, 2, 4 was mainly in the nucleus and cytoplasm while FGFR3 was predominantly localized in cytoplasm. CONCLUSIONS: FGFR shows co-expression in SCCHN cell lines, which may be associated with the tumorigenesis and development of SCCHN.

Key words: Fibroblast growth factor receptors, Squamous cell carcinoma, Head and neck

中图分类号:

R739.8

石超吉, 周榕, 韩永, 陶文杰, 孙树洋, 张志愿. 成纤维细胞生长因子受体家族在头颈鳞癌细胞中的表达及定位[J]. 上海口腔医学, 2016, 25(5): 517-521.

SHI Chao-ji, ZHOU Rong, HAN Yong, TAO Wen-jie, SUN Shu-yang, ZHANG Zhi-yuan. Expression and localization of FGFR family in squamous cell carcinoma of head and neck[J]. Shanghai Journal of Stomatology, 2016, 25(5): 517-521.

参考文献

[1] Turner N, Grose R. Fibroblast growth factor signalling: from development to cancer[J]. Nat Rev Cancer, 2010, 10(2): 116-129.
[2] Dieci MV, Arnedos M, Andre F, et al. Fibroblast growth factor receptor inhibitors as a cancer treatment: from a biologic rationale to medical perspectives [J]. Cancer Discov, 2013, 3(3): 264-279.
[3] Grose R, Dickson C. Fibroblast growth factor signaling in tumorigenesis[J]. Cytokine Growth Factor Rev, 2005, 16(2): 179-186.
[4] Haddad RI, Shin DM. Recent advances in head and neck cancer [J]. N Engl J Med, 2008, 359(11): 1143-1154.
[5] Brooks AN, Kilgour E, Smith PD. Molecular pathways: fibroblast growth factor signaling: a new therapeutic opportunity in cancer [J]. Clin Cancer Res, 2012, 18(7): 1855-1862.
[6] Greenman C, Stephens P, Smith R, et al. Patterns of somatic mutation in human cancer genomes [J]. Nature, 2007, 446(7132): 153-158.
[7] Hagel M, Miduturu C, Sheets M, et al. First selective small molecule inhibitor of FGFR4 for the treatment of hepatocellular carcinomas with an activated FGFR4 signaling pathway[J]. Cancer Discov, 2015, 5(4): 424-437.
[8] Gavine PR, Mooney L, Kilgour E, et al. AZD4547: an orally bioavailable, potent, and selective inhibitor of the fibroblast growth factor receptor tyrosine kinase family[J]. Cancer Res, 2012, 72(8): 2045-2056.
[9] Vairaktaris E, Ragos V, Yapijakis C, et al. FGFR-2 and -3 play an important role in initial stages of oral oncogenesis [J]. Anticancer Res, 2006, 26(6B): 4217-4221.
[10] Nguyen PT, Tsunematsu T, Yanagisawa S, et al. The FGFR1 inhibitor PD173074 induces mesenchymal-epithelial transition through the transcription factor AP-1[J]. Br J Cancer, 2013, 109(8): 2248-2258.
[11] Zhang Y, Hiraishi Y, Wang H, et al. Constitutive activating mutation of the FGFR3b in oral squamous cell carcinomas [J]. Int J Cancer, 2005, 117(1): 166-168.
[12] Kim HS, Lee SE, Bae YS, et al. Fibroblast growth factor receptor 1 gene amplification is associated with poor survival in patients with resected esophageal squamous cell carcinoma[J]. Oncotarget, 2015, 6(4): 2562-2572.
[13] Göke F, Bode M, Franzen A, et al. Fibroblast growth factor receptor 1 amplification is a common event in squamous cell carcinoma of the head and neck[J]. Mod Pathol, 2013, 26(10): 1298-1306.
[14] Ipenburg NA, Koole K, Liem KS, et al. Fibroblast growth factor receptor family members as prognostic biomarkers in head and heck squamous cell carcinoma: A systematic review [J]. Target Oncol, 2016, 11(1): 17-27.
[15] Dutra RL, de Carvalho MB, Dos Santos M, et al. FGFR4 profile as a prognostic marker in squamous cell carcinoma of the mouth and oropharynx [J]. PLoS One, 2012, 7(11): e50747.
[16] Streit S, Bange J, Fichtner A, et al. Involvement of the FGFR4 Arg388 allele in head and neck squamous cell carcinoma [J]. Int J Cancer, 2004, 111(2): 213-217.
[17] Murase H, Inokuchi M, Takagi Y, et al. Prognostic significance of the co-overexpression of fibroblast growth factor receptors 1, 2 and 4 in gastric cancer [J]. Mol Clin Oncol, 2014, 2(4): 509-517.