慢性牙周炎患者治疗前、后血清降钙素基因相关肽水平检测的临床意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (4): 448-452.

慢性牙周炎患者治疗前、后血清降钙素基因相关肽水平检测的临床意义

晏莺^{1, 2}, 向学熔^{1, 2}, 王春^{1, 2}, 叶国^{1, 2}, 范小平^{2, 3}, 杨梅^{1, 2}

1.重庆医科大学附属口腔医院牙周病科,重庆 401147; 2.口腔疾病与生物医学重庆市重点实验室,重庆 401147; 3.重庆医科大学附属口腔医院正畸科,重庆 401147

收稿日期:2016-03-25 出版日期:2016-08-25 发布日期:2016-09-06
通讯作者: 向学熔,E-mail:cqzxfxp@sina.com
作者简介:晏莺（1990- ）,女,硕士,E-mail:1436997194@qq.com
基金资助:
重庆市渝中区科技计划项目（20130138）; 重庆市渝北区科技计划项目[2015（社）10号]; 重庆市科技委员会计划项目（cstc2014jcyjA10055）

Clinical significance of calcitonin gene-related peptide level before and after treatment in patients with chronic periodontitis

YAN Ying^{1, 2}, XIANG Xue-rong^{1, 2}, WANG Chun^{1, 2}, YE Guo^{1, 2}, FAN Xiao-ping^{2, 3}, YANG Mei^{1, 2}

1. Department of Periodontology, Stomatological Hospital of Chongqing Medical University. Chongqing 401147;
2.Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences. Chongqing 401147;
3.Department of Orthodontics, Stomatological Hospital of Chongqing Medical University. Chongqing 401147, China

Received:2016-03-25 Online:2016-08-25 Published:2016-09-06

摘要/Abstract

摘要： 目的探讨慢性牙周炎患者治疗前、后血清中降钙素基因相关肽（calcitonin gene-related peptide,CGRP）水平的变化及意义。方法随机选择 2014 年 8 月—2015 年 6月在我院牙周科就诊的健康对照组30例,轻度、中度、重度牙周炎患者各30例,抽取空腹外周静脉血,离心、分离上清液,运用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测 CGRP浓度。慢性牙周炎患者轻度、中度、重度3组分别于基础治疗后3个月再次检测 CGRP浓度。采用SPSS 22.0软件包对数据进行统计学分析。结果ELISA检测结果显示,健康组静脉血中CGRP的含量显著高于牙周炎患者。随着牙周炎炎症程度加重,静脉血中CGRP的含量依次降低,重度牙周炎患者的含量最低,差异显著（P<0.01）;轻度、中度、重度牙周炎患者基础治疗3个月后,血清中CGRP含量相比同组治疗前显著升高（P<0.05）,治疗后牙周炎患者组间CGRP含量相比差异无显著性（P>0.05）。结论静脉血中CGRP的含量与慢性牙周炎存在相关性,其含量随慢性牙周炎逐渐加重而降低,推测CGRP可能参与慢性牙周炎的发生、发展过程。慢性牙周炎治疗后血清中CGRP含量明显增加,CGRP可作为慢性牙周炎患者治疗效果的临床检测指标。

关键词: 降钙素基因相关肽, 慢性牙周炎, 酶联免疫吸附法

Abstract: PURPOSE: To explore the clinical significance of calcitonin gene-related peptide (CGRP) levels in patients with chronic periodontitis before and after treatment, and to detect the calcitonin gene-related peptide content in human venous blood. METHODS: Thirty healthy controls and thirty patients with mild, moderate, severe periodontitis were enrolled from August 2014 to June 2015.CGRP level in the patients' peripheral blood was detected by ELISA. Three months after periodontal treatment, CGRP level in mild, moderate, severe periodontitis patients' peripheral blood was re-examined by ELISA. Then the correlation between calcitonin gene-related peptide and inflammation of chronic periodontitis was analyzed with SPSS 22.0 software package. RESULTS: The content of CGRP in healthy controls was significantly higher than that in patients with periodontitis. With the aggravation of periodontal inflammation, blood level of CGRP decreased gradually, and the lowest was in patients with severe periodontitis (P<0.01). Three months after periodontal treatment, CGRP content was significantly higher compared with that before treatment (P<0.05), but no significant difference was found in patients with different degree of periodontitis (P>0.05). CONCLUSIONS: The level of CGRP in venous blood decreased with the increasing severity of chronic periodontitis, and CGRP was negatively correlated with the degree of inflammation of chronic periodontitis. CGRP may be involved in the occurrence and development of chronic periodontitis. CGRP content in serum of patients with chronic periodontitis after treatment was significantly increased, CGRP may be used as the basis for clinical detection of chronic periodontitis.

Key words: Calcitonin gene-related peptide, Chronic periodontitis, Enzyme-linked immunosorbent assay

中图分类号:

R781.42

晏莺, 向学熔, 王春, 叶国, 范小平, 杨梅. 慢性牙周炎患者治疗前、后血清降钙素基因相关肽水平检测的临床意义[J]. 上海口腔医学, 2016, 25(4): 448-452.

YAN Ying, XIANG Xue-rong, WANG Chun, YE Guo, FAN Xiao-ping, YANG Mei. Clinical significance of calcitonin gene-related peptide level before and after treatment in patients with chronic periodontitis[J]. Shanghai Journal of Stomatology, 2016, 25(4): 448-452.

参考文献

[1] 孟焕新. 牙周病学 [M]. 第3版. 北京: 人民卫生出版社, 2008: 156-158.
[2] 石丽萍, 何艳艳, 王丽. 大鼠实验性牙周炎治疗前后血清中IL-18与sTREM-1的浓度变化[J].口腔医学研究, 2015, 31(6): 591-593.
[3] 宁婉辰, 乔聪聪, 王丽. 黑龙江地区PECAM-1多态性与慢性牙周炎的相关研究 [J].口腔医学研究,2015,31(2): 167-170.
[4] Lee HJ, Jeong GS, Pi SH, et al. Heme oxygenase-1 protects human periodontal ligament cells against substance P-induced RANKL expression [J]. J Periodontal Res, 2010, 45(3): 367-374.
[5] Yan XZ, Ge SH, Sun QF, et al. A pilot study evaluating the effect of recombinant human bone morphogenetic protein-2 and recombinant human beta-nerve growth factor on the healing of Class Ⅲ furcation defects in dogs [J]. J Periodontol, 2010, 81(9): 1289-1298.
[6] 叶青. 降钙素基因相关肽5247单核苷酸多态性（A/C）对人牙周膜成纤维细胞生物学特性的影响 [D]. 上海: 第二军医大学, 2012.
[7] Shinbara H, Okubo M, Kimura K, et al. Participation of calcitonin gene related peptide released via axon reflex in the local increase in muscle blood flow following manual acupuncture[J]. Acupunct Med, 2013, 31(1): 81-87.
[8] Porseva V, Strelkov A, Shilkin V, et al. Age-related changes in sensory neurons containing calcitonin gene-related peptide under conditions of afferentation deficit in rats [J]. Ontogenez, 2012, 43(6): 405-412.
[9] 黄声富, 韩光丽, 程祥荣, 等. 大鼠正畸牙移动过程中牙周组织内降钙素基因相关肽的变化 [J]. 口腔医学研究, 2003, 19(2): 115-117.
[10] Sun Y, Tao R, Zhang M, et al. Expression of calcitonin gene-related peptide in rat pulp and periodontal tissues by indirect immunofluorescence method [J]. Monoclon Antib Immunodiagn Immunother, 2013, 32(6): 404-408.
[11] Li J, Kreicbergs A, Bergström J, et al. Site-specific CGRP innervation coincides with bone formation during fracture healing and modeling: A study in rat angulated tibia [J]. J Orthop Res, 2007, 25(9): 1204-1212.
[12] Wang L, Shi X, Zhao R, et al. Calcitonin-gene-related peptide stimulates stromal cell osteogenic differentiation and inhibits RANKL induced NF-κB activation, osteoclastogenesis and bone resorption [J]. Bone, 2010, 46(5): 1369-1379.
[12] Mrak E, Guidobono F, Moro G, et al. Calcitonin gene-related peptide(CGRP) inhibits apoptosis in human osteoblasts by β-catenin stabilization[J]. J Cell Physiol, 2010, 225(3): 701-708.
[13] Villa I, Mrak E, Rubinacci A, et al. CGRP inhibits osteoprotegerin production in human osteoblast-like cells via cAMP/PKA-dependent pathway [J]. Am J Physiol Cell Physiol, 2006, 291(3): C529-C537.
[14] Granholm S, Lundberg P, Lerner UH. Expression of the calcitonin receptor, calcitonin receptor-like receptor, and receptor activity modifying proteins during osteoclast differentiation [J]. J Cell Biochem, 2008, 104(3): 920-933.
[15] Lundy FT, Salmon AL, Lamey PJ, et al. Carboxypeptidase-mediated metabolism of calcitonin gene-related peptide in human gingival crevicular fluid-a role in periodontal inflammation? [J]. J Clin Periodontol, 2000, 27(7): 499-505.
[16] Mishima T, Ito Y, Hosono K, et al. Calcitonin gene-related peptide facilitates revascularization during hindlimb ischemia in mice [J]. Am J Physiol Heart Circ Physiol, 2011, 300(2): H431-H439.
[17] Suzuki A, Uemura T, Nakamura H. Control of bone remodeling by nervous system. Neural involvement in fracture healing and bone regeneration[J]. Clin Calcium, 2010, 20(12): 1820-1827.