IL-34在慢性根尖周病损组织中的表达及临床意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (1): 53-57.

IL-34在慢性根尖周病损组织中的表达及临床意义

马楠，曲柳，徐莉雅，于雅琼，仇丽鸿

中国医科大学口腔医学院牙体牙髓病科；
辽宁省口腔医学研究所牙体牙髓病学研究室；
辽宁省口腔疾病转化医学研究中心，辽宁沈阳 110002

收稿日期:2015-07-13 修回日期:2015-09-18 出版日期:2016-02-25 发布日期:2016-03-09
通讯作者: 仇丽鸿，Tel:024-22895932，E-mail:drqlh@yahoo.com E-mail:graduatemn1990cmu@163.com
作者简介:马楠(1990-)，女，硕士研究生，医师
基金资助:
沈阳市科学技术基金(F15-199-1-56)

Expression of IL-34 in chronic periapical lesions and its clinical significance

MA Nan, QU Liu, XU Li-ya, YU Ya-qiong, QIU Li-hong.

Department of Endodontics, School of Stomatology, China Medical University;
Lab of Endodontics, Liaoning Institute of Dental Research;
Research Center of Translational Medicine, Liaoning Provincial Institute of Oral Diseases. Shenyang 110002, Liaoning Province, China

Received:2015-07-13 Revised:2015-09-18 Online:2016-02-25 Published:2016-03-09

摘要/Abstract

摘要： 目的： 比较慢性根尖周病损组织和健康牙周膜组织中白细胞介素34(Interleukin-34, IL-34)的表达水平，探讨IL-34在慢性根尖周炎发病中的作用。方法： 收集25例诊断为慢性根尖周炎患牙的根尖周组织作为病例组，22例因正畸拔除的健康牙的牙周膜作为对照组，应用实时荧光定量PCR(real-time PCR, RT-PCR)检测IL-34mRNA的表达；应用免疫组织化学法检测IL-34蛋白的表达，采用图像分析软件检测病例组中IL-34蛋白的表达水平。采用SPSS13.0软件包对数据进行统计学分析。结果： IL-34mRNA在慢性根尖周炎中的表达水平(3.53±3.07)显著高于对照组(1.07±0.76)，IL-34蛋白在慢性根尖周病损组织中阳性表达，主要定位于淋巴细胞、浆细胞及巨噬细胞中，且病例组中IL-34蛋白表达水平显著高于对照组(P<0.01)。结论： IL-34可能与慢性根尖周炎症密切相关。

关键词: 白细胞介素34, 慢性根尖周炎, 实时定量PCR, 免疫组织化学

Abstract: PURPOSE: To observe the expression of IL-34 mRNA in chronic periapical lesions and healthy periodontal ligaments and discuss the role of IL-34 in the etiology of chronic apical periodontitis. METHODS： A total of 25 periapical tissues from chronic apical periodontitis and 22 normal periodontal ligament tissue from extracted healthy teeth for orthodontic reason were selected. The expression of IL-34mRNA was detected by real-time PCR; the expression of IL-34 protein was detected by immunohistochemical analysis. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: The level of IL-34 mRNA expression in periapical lesions (3.53±3.07) was significantly higher than that of the normal control (1.07±0.76); IL-34 was positively expressed in lymphocytes, plasma cells and macrophages. Image analysis software indicated that the level of IL-34 protein was significantly higher in periapical lesions than that in normal control (P<0.01). CONCLUSIONS: IL-34 may be closely related to inflammation of chronic apical periodontitis.

Key words: IL-34, Chronic apical periodontitis, RT-PCR, Immunohistochemstry

中图分类号:

R781.34

马楠，曲柳，徐莉雅，于雅琼，仇丽鸿. IL-34在慢性根尖周病损组织中的表达及临床意义[J]. 上海口腔医学, 2016, 25(1): 53-57.

MA Nan, QU Liu, XU Li-ya, YU Ya-qiong, QIU Li-hong.. Expression of IL-34 in chronic periapical lesions and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(1): 53-57.

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