胶原蛋白海绵对大鼠拔牙后牙槽窝愈合过程的影响

doi:10.19439/j.sjos.2023.03.004

上海口腔医学 ›› 2023, Vol. 32 ›› Issue (3): 241-245.doi: 10.19439/j.sjos.2023.03.004

胶原蛋白海绵对大鼠拔牙后牙槽窝愈合过程的影响

李俊福¹, 朱佳², 姜娟¹, 王旭霞³, 陈岱韻⁴, 赵作勤⁴

1.山东第一医科大学第二附属医院口腔科,山东泰安 271000;
2.湖州市南太湖医院口腔科,浙江湖州 313000;
3.山东大学齐鲁医学院口腔医学院·口腔医院口腔颌面外科,山东省口腔组织再生重点实验室, 山东省口腔生物材料与组织再生工程实验室,山东济南 250012;
4.山东第一医科大学口腔医学院,山东泰安 271000

收稿日期:2021-11-25 修回日期:2022-05-20 出版日期:2023-06-25 发布日期:2023-06-28
通讯作者: 赵作勤,E-mail：zzq458@163.com
作者简介:李俊福（1979-）,男,硕士,副主任医师,E-mail：xiaoshaguadt@126.com
基金资助:
泰安市科技创新计划项目（2020NS093）

Effect of collagen sponge on the healing process of alveolar fossa after tooth extraction in rats

LI Jun-fu¹, ZHU Jia², JIANG Juan¹, WANG Xu-xia³, CHEN Dai-yun⁴, ZHAO Zuo-qin⁴

1. Department of Stomatology, Second Affiliated Hospital of Shandong First Medical University. Tai'an 271000, Shandong Province;
2. Department of Stomatology, South Taihu Hospital. Huzhou 313000, Zhejiang Province;
3. Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University; Shandong Key Laboratory of Oral Tissue Regeneration; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Shandong University. Jinan 250012, Shandong Province;
4. School of Stomatology, Shandong First Medical University. Tai'an 271000, Shandong Province, China

Received:2021-11-25 Revised:2022-05-20 Online:2023-06-25 Published:2023-06-28

摘要/Abstract

摘要： 目的：探讨胶原蛋白海绵对拔牙创牙槽窝愈合过程的影响。方法：取健康雌性SD大鼠16只,首先建立大鼠拔牙模型,实验侧（右）牙槽窝内填入胶原蛋白海绵,对照侧（左）作空白处理。于拔牙术后1、2、4、8周处死大鼠,观察牙槽窝成骨情况,利用实时定量PCR检测实验侧与对照侧成骨相关基因表达变化。采用SPSS 19.0软件包进行统计学分析。结果：术后实验侧牙槽窝较对照侧愈合明显好,实验侧及对照侧组织中Osterix、Runx2及Vegf均有表达,实验侧组织相关基因在术后1、2、4、8周不同时间点的表达水平显著高于对照侧（P<0.05）。结论：胶原蛋白海绵填塞拔牙创牙槽窝可在一定程度上促进牙槽窝早期骨愈合,这一过程可能与胶原蛋白海绵调节成骨相关基因表达水平有关。

关键词: 胶原蛋白海绵, 牙槽窝, 基因表达, 实时定量PCR

Abstract: PURPOSE: To investigate the effect of collagen sponge on early bone healing process of alveolar fossa after tooth extraction in rats. METHODS: A total of 16 healthy female SD rats were selected. Animal models with tooth extraction were established. The right alveolar fossa inserted with collagen sponge was as the experimental group, and the left alveolar fossa was as the control group with treatment. The rats were sacrificed 1, 2, 4 and 8 weeks after tooth extraction, and the osteogenesis of alveolar fossa was observed. Real-time quantitative PCR (qt-PCR) was used to detect the changes of osteogenesis related gene expression. SPSS 19.0 software package was used for statistical analysis. RESULTS: After surgery, alveolar cavity healing was significantly better in the experimental group than in the control group. Osterix, Runx2 and Vegf genes were expressed in the experimental group and the control group, and the expression levels of related genes in the experimental group were significantly higher than the control group 1, 2, 4 and 8 weeks after surgery(P<0.05). CONCLUSIONS: Collagen sponge could promote early alveolar bone healing, possibly related to the expression level of osteogenic genes regulated by collagen sponge.

Key words: Collagen sponge, Alveolar fossa, Gene expression, Real-time quantitative PCR

中图分类号:

R782.1

李俊福, 朱佳, 姜娟, 王旭霞, 陈岱韻, 赵作勤. 胶原蛋白海绵对大鼠拔牙后牙槽窝愈合过程的影响[J]. 上海口腔医学, 2023, 32(3): 241-245.

LI Jun-fu, ZHU Jia, JIANG Juan, WANG Xu-xia, CHEN Dai-yun, ZHAO Zuo-qin. Effect of collagen sponge on the healing process of alveolar fossa after tooth extraction in rats[J]. Shanghai Journal of Stomatology, 2023, 32(3): 241-245.

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胶原蛋白海绵对大鼠拔牙后牙槽窝愈合过程的影响

Effect of collagen sponge on the healing process of alveolar fossa after tooth extraction in rats

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