烤瓷牙修复对龈沟液内IL-23表达水平的影响

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (1): 58-62.

烤瓷牙修复对龈沟液内IL-23表达水平的影响

方絮，靳赢，高科北，王玉，林晓萍

中国医科大学附属盛京医院口腔科，辽宁沈阳 110004

收稿日期:2015-01-26 修回日期:2015-03-26 出版日期:2016-02-25 发布日期:2016-03-09
通讯作者: 林晓萍， Tel: 024-96615-61522，E-mail:HXiaoping_ba@yahoo.com E-mail:124033104@qq.com
作者简介:方絮（1987- ），女，硕士研究生
基金资助:
高等学校博士学科点专项科研基金 (20112104110013)；辽宁省科学技术计划项目（2012225015）；沈阳市科学技术项目计划（F13-221-9-28）

The influence of porcelain-fused-to-metal on the expression level of IL-23 in gingival crevicular fluid

FANG Xu, JIN Ying, GAO Ke-bei, WANG Yu, LIN Xiao-ping.

Department of Stomatology, Shengjing Hospital of China Medical University. Shenyang 110004, Liaoning Province, China

Received:2015-01-26 Revised:2015-03-26 Online:2016-02-25 Published:2016-03-09

摘要/Abstract

摘要： 目的： 探讨IL-23在牙周炎发病机制中的作用及烤瓷牙修复对牙周组织中IL-23表达水平的影响。方法： 选取正常组10例，牙龈炎组14例，烤瓷熔附金属全冠（PFM）牙龈炎组14例和PFM牙周炎组14例病例，采用Florida牙周探针探查各受试牙，记录临床指标（GI、SBI、AL、PD）后采集龈沟液，用双抗体夹心酶联免疫吸附试验(ELISA)检测龈沟液中IL-23的表达水平，采用SPSS13.0软件包对数据进行统计学分析。结果： 正常组龈沟液中IL-23表达水平（227.047±29.880）pg/mL低于炎性组，PFM牙周炎组（511.327±138.846）pg/mL高于PFM牙龈炎组（347.359±218.260）pg/mL(P<0.05)，较牙龈炎组（330.353±196.266）pg/mL差异显著(P<0.01)；IL-23的表达水平与临床指标（GI、SBI、AL、PD）间呈正相关关系（Pearson相关系数r>0.4，P<0.05），PFM牙龈炎组牙周破坏程度重于牙龈炎组，AL有显著差异(P<0.05)，PD差异显著(P<0.01)，且PD与IL-23表达水平变化关系最为密切，差异最为显著（Pearson相关系数r>0.5，P<0.01）。结论： IL-23 在牙周炎发病机制中发挥重要作用，烤瓷牙修复加重牙周组织破坏并影响IL-23表达水平。

关键词: IL-23, 烤瓷牙修复, 龈沟液, 牙周炎

Abstract: PURPOSE: To investigate the role of IL-23 in the pathogenesis of periodontitis, and the influence of porcelain-fused-to-metal (PFM) on periodontal tissue and the expression level of IL-23. METHODS： The gingival crevicular fluid (GCF) samples were collected in 10 periodontally healthy individuals, 14 cases with gingivitis, 14 cases with gingivitis and PFM, and 14 cases with periodontitis and PFM. The teeth were detected and recorded the clinical parameters（GI, SBI, AL, PD）by Florida probe. The concentration of IL-23 in GCF was detected by ELISA assays.SPSS13.0 software package was used for statistical analysis. RESULTS: The expression of IL-23 in GCF of normal group (227.047±29.880)pg/mL was below inflammatory groups. A significant overexpression of IL-23 in GCF was detected in periodontitis with PFM group(511.327±138.846)pg/mL compared to gingivitis group (330.353±196.266)pg/mL (P<0.01) and gingivitis with PFM group (347.359±218.260)pg/mL (P<0.05). The expression level of IL-23 in GCF was positively correlated with clinical parameters (GI, SBI, AL, PD) (r>0.4, P<0.05). There was a high degree of periodontal destruction in gingivitis with PFM group compared to gingivitis group, and significant difference existed in AL (P<0.05) and PD (P<0.01). The change of IL-23 expression was most positively correlated with PD (r>0.5, P<0.01). CONCLUSIONS: IL-23 might play an important role in the pathogenesis of periodontitis. PFM could have influence on the destruction of periodontal tissues and the expression level of IL-23.

Key words: IL-23, Porcelain-fused-to-metal, Gingival crevicular fluid, Periodontitis

中图分类号:

R781.4

方絮，靳赢，高科北，王玉，林晓萍. 烤瓷牙修复对龈沟液内IL-23表达水平的影响[J]. 上海口腔医学, 2016, 25(1): 58-62.

FANG Xu, JIN Ying, GAO Ke-bei, WANG Yu, LIN Xiao-ping.. The influence of porcelain-fused-to-metal on the expression level of IL-23 in gingival crevicular fluid[J]. Shanghai Journal of Stomatology, 2016, 25(1): 58-62.

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