IL-35在慢性牙周炎血清及龈沟液中的表达及相关性

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 455-459.

IL-35在慢性牙周炎血清及龈沟液中的表达及相关性

刘迪昕, 靳赢, 林晓萍

中国医科大学附属盛京医院口腔科,辽宁沈阳 110004

收稿日期:2014-07-02 出版日期:2015-08-20 发布日期:2015-09-10
作者简介:刘迪昕（1988-）,女,硕士研究生,
基金资助:
高等学校博士学科点专项科研基金 (20112104110013)

Expression and correlation of IL-35 in gingival crevicular fluid and serum with chronic periodontitis

LIU Di-xin, JIN Ying, LIN Xiao-ping

Department of Stomatology, Shengjing Hospital of China Medical University. Shenyang 110004, Liaoning Province, China

Received:2014-07-02 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by Higher School Specialized Research Fund for Doctoral Program (20112104110013) and Science and Technology Plan Project of Liaoning Province (2012225015)

摘要/Abstract

摘要： 目的通过检测牙周炎患者血清及龈沟液中白细胞介素35( IL-35)的水平,分析其与临床指标的相关性,初步探讨IL-35在牙周疾病过程中的变化情况。方法按纳入标准选择20例中、重度牙周炎患者作为实验组,20例健康人群作为对照组;记录患者的一般信息、牙周袋探诊深度( PD)和临床附着丧失( CAL) 情况;收集静脉血清及龈沟液样本,采用酶联免疫吸附法(ELISA)测定血清及龈沟液中的IL-35水平;利用SPSS19.0软件包进行Pearson秩相关检验,分析IL-35的表达及与临床指标的关系。结果实验组血清及龈沟液中IL-35平均浓度分别为（330.42±19.23）ng/mL、(205.56±14.42) ng/mL,对照组分别为(206.89±45.01) ng/mL、(101.88±3.34 ) ng/mL,实验组血清及龈沟液中IL-35水平显著高于对照组( P<0.01)。实验组血清及龈沟液中IL-35水平分别与PD和CAL呈负相关。结论慢性牙周炎可导致血清及龈沟液中IL-35水平显著升高, 其升高程度与牙周炎呈负相关。

关键词: 白细胞介素35, 血清, 龈沟液, 慢性牙周炎, 全身性疾病

Abstract: PURPOSE: To investigate the concentration of IL-35 in serum and gingival crevicular fluid (GCF) and the correlation between IL-35 and periodontal clinical parameters, and explore the possible role of IL-35 in chronic periodontitis (CP). METHODS: Twenty patients with moderate or severe chronic periodontitis were selected as experimental group and 20 healthy people were included as control group. The patients’ baseline information, probing depth (PD) and clinical attachment level (CAL) were recorded. Their serum and GCF were collected and then the concentration of IL-35 in GCF and serum were detected by ELISA. SPSS19.0 software package was used for statistical analysis. RESULTS: The concentration of IL-35 in GCF and serum of chronic periodontitis were significantly higher than the control group (P<0.001). There was significant negative correlation between IL-35 and clinical parameters. CONCLUSIONS: Chronic periodontitis may increase the concentration of IL-35 in serum and GCF; IL-35 level is negatively correlated with periodontitis.

Key words: Interleukin 35, Serum, Gingival crevicular fluid, Chronic periodontitis, Systemic disease Shanghai J Stomatol, 2015, 24(4):455-459.

中图分类号:

R780.2

刘迪昕, 靳赢, 林晓萍. IL-35在慢性牙周炎血清及龈沟液中的表达及相关性[J]. 上海口腔医学, 2015, 24(4): 455-459.

LIU Di-xin, JIN Ying, LIN Xiao-ping. Expression and correlation of IL-35 in gingival crevicular fluid and serum with chronic periodontitis[J]. Shanghai Journal of Stomatology, 2015, 24(4): 455-459.

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