正畸应力变化下牙龈组织α-SMA、Ⅰ型和Ⅲ型胶原变化及龈沟液MMP-2、TIMP-2的表达

doi:10.19439/j.sjos.2021.04.012

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (4): 402-405.doi: 10.19439/j.sjos.2021.04.012

正畸应力变化下牙龈组织α-SMA、Ⅰ型和Ⅲ型胶原变化及龈沟液MMP-2、TIMP-2的表达

姜莉萍, 唐镇

南昌大学附属口腔医院正畸科, 江西省口腔生物医学重点实验室, 江西南昌 330006

收稿日期:2020-06-22 修回日期:2020-08-11 出版日期:2021-08-25 发布日期:2021-09-23
通讯作者: 唐镇,E-mail:836048616@qq.com
作者简介:姜莉萍（1985-）,女,硕士研究生,主治医师,E-mail:mkinhy001@163.com
基金资助:
江西省卫生计生委科技计划（20191071）

Changes of α-SMA, type I and type Ⅲ collagen in gingival tissue and expression of MMP-2 and TIMP-2 in gingival crevicular fluid under orthodontic force

JIANG Li-ping, TANG Zhen

Department of Orthodontics, Affiliated Stomatological Hospital of Nanchang University, Jiangxi Provincial Key Laboratory of Oral Biomedicine. Nanchang 330006, Jiangxi Province, China

Received:2020-06-22 Revised:2020-08-11 Online:2021-08-25 Published:2021-09-23

摘要/Abstract

摘要： 目的: 探讨正畸应力变化下牙龈组织α平滑肌肌动蛋白（α-SMA）、Ⅰ型及Ⅲ型胶原变化以及龈沟液基质金属蛋白酶2（MMP-2）、金属蛋白酶组织抑制因子2（TIMP-2）的表达。方法: 选取南昌大学附属口腔医院2018年4月—2019年4月收治的正畸患者74例,随机分为A组（24例,0 g力）、B组（25例,75 g力）、C组（25例,150 g力）3组。于加力0、4周采集患者部分牙龈组织,采用免疫组织化学染色法检测α-SMA、Ⅰ型及Ⅲ型胶原表达水平。在加力后0、2、4周收集龈沟液,采用酶联免疫吸附法检测MMP-2、TIMP-2表达水平。采用Spearman相关性分析不同正畸应力与α-SMA、Ⅰ型胶原、Ⅲ型胶原、MMP-2、TIMP-2表达水平的相关性。采用SPSS 19.0软件包对数据进行统计分析。结果: 加力2周及加力4周,3组患者龈沟液MMP-2、TIMP-2表达水平依次升高（P<0.05）;加力4周后,3组患者牙龈组织中α-SMA、Ⅰ型及Ⅲ型胶原水平依次升高（P<0.05）;不同正畸应力与MMP-2、TIMP-2、α-SMA、Ⅰ型胶原及Ⅲ型胶原表达水平均呈正相关（P<0.05）。结论: 龈沟液TIMP-2、MMP-2表达水平以及肌成纤维细胞表达与正畸应力变化有关,可能在正畸治疗的牙周组织改建中发挥重要作用。

关键词: 正畸应力, 龈沟液, α平滑肌肌动蛋白, 基质金属蛋白酶

Abstract: PURPOSE: To investigate the changes of α-smooth muscle actin (α-SMA), type Ⅰ and type Ⅲ collagen in gingival tissue and expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in gingival crevicular fluid under orthodontic force. METHODS: Seventy-four patients undergoing orthodontic treatment in Affiliated Stomatological Hospital of Nanchang University from April 2018 to April 2019 were enrolled, and randomly divided into three groups. Group A(n=24) received the treatment under 0 g of orthodontic force, group B (n=25) under 75 g of orthodontic force, and group C(n=25) under 150 g of orthodontic force. At the baseline and 4th week of treatment, the expression levels of α-SMA, type I collagen and type Ⅲ collagen in gingival tissues were detected by immunohistochemical staining. At the baseline, the 2nd, and 4th week of treatment, the expression levels of MMP-2 and TIMP-2 in gingival crevicular fluid were detected by enzyme-linked immunosorbent assay. Then the correlation between different orthodontic force and levels of α-SMA, type Ⅰ collagen, type Ⅲ collagen, MMP-2 and TIMP-2 was analyzed using Spearman correlation analysis. Statistical analysis was completed by SPSS 19.0 software package. RESULTS: At the 2nd and 4th week of treatment, the expression levels of MMP-2 and TIMP-2 in gingival crevicular fluid among three groups were the lowest in group A, followed by group B and group C(P<0.05). At the 4th week of treatment, the levels of α-SMA, type Ⅰ and type Ⅲ collagen in gingival tissues among three groups were the lowest in group A, followed by group B and group C(P<0.05). The orthodontic force was positively correlated with expression levels of α-SMA, type Ⅰ collagen, type Ⅲ collagen, MMP-2 and TIMP-2(P<0.05). CONCLUSIONS: The expression levels of MMP-2 and TIMP-2 in gingival crevicular fluid and myofibroblast are related to the changes of orthodontic force, which may play an important role in the reconstruction of periodontal tissue during orthodontic treatment.

Key words: Orthodontic force, Gingival crevicular fluid, α-smooth muscle actin, Matrix metalloproteinase

中图分类号:

R783.5

姜莉萍, 唐镇. 正畸应力变化下牙龈组织α-SMA、Ⅰ型和Ⅲ型胶原变化及龈沟液MMP-2、TIMP-2的表达[J]. 上海口腔医学, 2021, 30(4): 402-405.

JIANG Li-ping, TANG Zhen. Changes of α-SMA, type I and type Ⅲ collagen in gingival tissue and expression of MMP-2 and TIMP-2 in gingival crevicular fluid under orthodontic force[J]. Shanghai Journal of Stomatology, 2021, 30(4): 402-405.

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正畸应力变化下牙龈组织α-SMA、Ⅰ型和Ⅲ型胶原变化及龈沟液MMP-2、TIMP-2的表达

Changes of α-SMA, type I and type Ⅲ collagen in gingival tissue and expression of MMP-2 and TIMP-2 in gingival crevicular fluid under orthodontic force

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