rhPDGF-BB与rhTGF-β1联合应用对大鼠正畸牙破骨细胞 FAK蛋白表达的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 423-427.

rhPDGF-BB与rhTGF-β₁联合应用对大鼠正畸牙破骨细胞 FAK蛋白表达的影响

张疆弢, 梅梅, 江策, 丰雷, 黄瑾, 王家佳

遵义医学院附属口腔医院正畸科,贵州遵义 563003

收稿日期:2014-10-13 出版日期:2015-08-20 发布日期:2015-09-10
通讯作者: 张疆弢,Tel:0851-28635725,E-mail：zhangjiangtao75@sina.com E-mail:zhangjiangtao75@sina.com
作者简介:张疆弢（1975-）,女,硕士,副主任医师
基金资助:
贵州省科学技术基金 [黔科合J字LKZ(2011)05号]

The influence of combined use of rhPDGF-BB and rhTGF-β₁ on protein expression of FAK in osteoclast during orthodontic tooth movement in rats

ZHANG Jiang-tao, MEI Mei, JIANG Ce, FENG Lei, HUANG Jin, WANG Jia-jia

Department of Orthodontics, Zunyi Medical College Affiliated Stomatology Hospital. Zunyi 563003, Guizhou Province, China

Received:2014-10-13 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by Science and Technology Foundation of Guizhou Province [LKZ(2011)05]

摘要/Abstract

摘要： 目的探讨重组人血小板衍生生长因子BB (rhPDGF-BB)与转化生长因子β₁(rhTGF-β₁)联合应用对大鼠正畸牙压力侧破骨细胞FAK蛋白的表达变化。方法160只SD大鼠随机分为实验组(A)和对照组(B)。每组根据检测时间点不同分为5个亚组,分别建立正畸牙移动模型。A组从加力当天开始,隔天注射rhPDGF-BB与rhTGF-β₁混合液 0.1 mL。加力后1、4、7、10、14 d,分别处死每组中1亚组大鼠。体式显微镜测量牙移动距离的改变,TRAP染色观察压力侧破骨细胞数量变化,免疫组织化学染色法检测FAK蛋白水平的表达变化。采用SPSS17.0软件包对数据进行统计学分析。结果A组牙移动距离均大于B组,除加力第1天无显著差异（P>0.05）外,其余各时间点差异均具有显著性（P<0.05）;A组破骨细胞数目增加较B组明显,除第14天无显著差异外,其余各时间点均具有显著差异(P<0.05);A组破骨细胞内FAK蛋白表达明显高于B组,任何时间点相比,均具有显著差异(P<0.05)。结论rhPDGF-BB和rhTGF-β₁的联合协同作用上调了正畸牙压力侧破骨细胞内FAK蛋白的表达,进一步促进破骨细胞的分化、增殖及骨吸收,从而加速正畸牙的移动速率。

关键词: 血小板衍生生长因子BB, 转化生长因子β, 1, 正畸牙, 破骨细胞, FAK

Abstract: PURPOSE: To explore the combined effects of recombinant human platelet-derived growth factor-BB(rhPDGF-BB)and recombinant human transforming growth factor -β₁ (rhTGF-β₁) on protein expression of FAK in osteolasts of the pressure side of orthodontic tooth in rats. METHODS: One hundred and sixty male Sprague-Dawley rats were randomly divided into 2 groups: experimental group(A) and control group(B). Each group was subdivided equally into 5 subgroups. Orthodontic tooth movement model was established. The experimental group received injection of 0.1 mL solution, containing 10 ng rhPDGF-BB and 5 ng rhTGF-β₁ every other day from day 1. Rats in each subgroup were sacrificed at each of five time points(1, 4, 7, 10 and 14 days) after appliance placement. The distances of the teeth movement were measured using stereomicroscope, and the changes of the amount of osteoclasts were detected by using tartrate resistant acid phosphatase histochemistry (TRAP). The protein expressions of FAK were assayed by immunohistochemical technique. SPSS17.0 software package was used for statistics analysis. RESULTS: Teeth in group A moved more rapidly than group B, and there were significant differences between 2 groups (P<0.05) except subgroup A1 and B1(P>0.05). The number of osteoclasts in group A was significantly higher than in group B. Except at 14 days, significant differences existed in between other time points (P<0.05). The protein expression of FAK in group A was significantly higher than in group B at any time points. CONCLUSIONS: The combined effects of rhPDGF-BB and rhTGF-β₁ up-regulate the protein expression of FAK within osteoclast in the pressure side, further promoted osteoclast differentiation, proliferation and bone resorption, thusaccelerate the rate of orthodontic tooth movement.

Key words: rhPDGF-BB, rhTGF-β1, Orthodontic tooth, Osteoclasts, FAK Shanghai J Stomatol, 2015, 24(4):423-427.

中图分类号:

R780.2

张疆弢, 梅梅, 江策, 丰雷, 黄瑾, 王家佳. rhPDGF-BB与rhTGF-β₁联合应用对大鼠正畸牙破骨细胞 FAK蛋白表达的影响[J]. 上海口腔医学, 2015, 24(4): 423-427.

ZHANG Jiang-tao, MEI Mei, JIANG Ce, FENG Lei, HUANG Jin, WANG Jia-jia. The influence of combined use of rhPDGF-BB and rhTGF-β₁ on protein expression of FAK in osteoclast during orthodontic tooth movement in rats[J]. Shanghai Journal of Stomatology, 2015, 24(4): 423-427.

参考文献

[1] 钟建莉. rhPDGF-BB对大鼠正畸牙移动过程中破骨细胞FAK表达的影响 [D].遵义: 遵义医学院, 2009.
[2] 刘亚, 王小琴, 马艳宁. 血小板衍生生长因子BB和转化生长因子β 1 联合应用对大鼠正畸牙移动的影响[J]. 中国药物与临床, 2013, 13(6): 719-721.
[3] Mukherjee A, Rotwein P. Selective signaling by Akt1 controls osteoblast differentiation and osteoblast-mediated osteoclast development [J]. Mol Cell Biol, 2012, 32 (2): 490-500.
[4] Sant'Ana AC, Marques MM, Barroso TE, et al. Effect of TGF-β 1 ,PDGF-BB and IGF-1 on the rate of proliferation and adhesion of a periodontal ligament cell lineage in vitro [J].J Periodontal, 2007, 78(10): 2007-2017.
[5] Belal MH, Watanabe H, Ichinose S, et al. Effect of PDGF-BB combined with EDTA gel on adhesion and proliferation to the root surface [J]. Odontology, 2012, 100(2): 206-214.
[6] Li X, McFarland DC, Velleman SG. Transforming growth factor-β 1 -induced satellite cell apoptosis in chickens is associated with β 1 integrin-mediated focal adhesion kinase activation[J]. Poult Sci,2009, 88(8): 1725-1734.
[7] Cooke JW, Sarment DP, Whitesman LA, et al. Effect of rhPDGF-BB delivery on mediators of periodontal wound repair[J]. Tissue Eng, 2006, 12(6): 1441-1450.
[8] 黄瑾, 刘建国, 宋琦, 等. 重组人血小板衍生生长因子BB与转化生长因子β 1 联合应用对大鼠正畸牙牙周组织改建的影响 [J]. 中华口腔医学杂志, 2013, 48(z1): 72-76.
[9] Han J, Meng HX, Tang JM, et al. The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro [J].Cell Prolif,2007,40(2): 241-252.
[10] Schaller MD. Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions[J]. J Cell Sci, 2010, 123(Pt7): 1007-1013.
[11] Ray BJ, Thomas K, Huang CS, et al. Regulation of osteoclast structure and function by FAK family kinases [J]. J Leukoc Biol, 2012, 92(5): 1021-1028.
[12] Ivankovic-Dikic I, Gronroos E, Blaukat A, et al. Pyk2 and FAK regulate neurite outgrowth induced by growth factors and integrins [J]. Nat Cell Biol, 2000, 2(9): 574-581.
[13] Campbell M, Trimble ER. Modification of PI3K-and MAPK-dependent chemotaxis in aortic vascular smooth muscle cells by protein kinase CbetaII [J].Circ Res, 2005, 96(2): 197-206.
[14] Genua M, Xu SQ, Buraschi S, et al. Proline-rich tyrosine kinase 2 (Pyk2) regulates IGF-I-Induced cell motility and invasion of urothelial carcinoma cells [J]. PLoS One, 2012, 7(6): e40148.
[15] Bolós V, Gasent JM, López-Tarruella S, et al. The dual kinase complex FAK-Src as a promising therapeutic target in cancer [J]. Onco Targets Ther, 2010, 3: 83-97.

rhPDGF-BB与rhTGF-β₁联合应用对大鼠正畸牙破骨细胞 FAK蛋白表达的影响

The influence of combined use of rhPDGF-BB and rhTGF-β₁ on protein expression of FAK in osteoclast during orthodontic tooth movement in rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张中月, 陈丽, 闫娜, 张淑华. 激光联合牙周基础治疗对牙周炎患者牙周指标、龈下菌群及龈沟液脂联素、MMP-13、IL-1β的影响[J]. 上海口腔医学, 2024, 33(3): 295-300.
[2]	郁莹, 翁清清, 罗银月, 姚姝冉, 易芳羽, 张颖. 过量氟对小鼠髁突软骨损伤及自噬相关因子LC3、p62表达的影响[J]. 上海口腔医学, 2024, 33(2): 113-116.
[3]	陈宏丽, 尹刚, 张海娟. 阿托伐他汀促进大鼠牙槽骨缺损愈合及对Wnt/β-catenin信号通路的影响[J]. 上海口腔医学, 2024, 33(2): 130-134.
[4]	王静, 徐娜, 任慧迪. TNF-α通过ERK1/2-Runx2信号通路调控SHED成骨分化能力的实验研究[J]. 上海口腔医学, 2024, 33(2): 135-140.
[5]	赵开, 张宇宁, 纪帅旗, 刘颖, 姜涵. CFL-1在头颈部鳞癌中的表达与患者预后的关系[J]. 上海口腔医学, 2024, 33(2): 148-155.
[6]	刘琳, 息雪娜, 黄涛, 韩国良. 基质细胞衍生因子1α联合自体贫血小板血浆在恒牙撕脱伤中的应用评价[J]. 上海口腔医学, 2024, 33(2): 175-179.
[7]	郝妍, 白相宇, 霍峰, 陈喜波. 下调SETDB1通过SOX7甲基化抑制口腔癌上皮-间充质转化、侵袭和转移的机制探讨[J]. 上海口腔医学, 2024, 33(1): 30-35.
[8]	段咏华, 吴开柳, 梅健, 周谊渊, 刘梦石. 口腔鳞癌组织中RACK1与EGP40的表达及其与临床病理特征及预后的相关性[J]. 上海口腔医学, 2023, 32(4): 385-390.
[9]	李欣然, 陈彬, 李梦, 杜琛, 孟箭. PAR指数联合头影测量在骨性Ⅲ类错畸形疗效评价中的初步应用[J]. 上海口腔医学, 2023, 32(4): 417-421.
[10]	李宗谕, 李晓琳, 尉鹏功, 曲柳, 仇丽鸿, 于雅琼. 白藜芦醇在人牙髓干细胞成牙本质向分化中的调控机制研究[J]. 上海口腔医学, 2023, 32(2): 132-136.
[11]	谢雪梅, 张佳园, 张野, 俞晓佳, 刘李明, 王健, 于德栋. 龈沟液中sICAM-1、IL-1β和HIF-1α水平与义齿种植修复并发种植体周围炎的关系[J]. 上海口腔医学, 2023, 32(1): 47-51.
[12]	魏军水, 孙鑫, 徐金标, 陈逸达. LncRNA RUNX1-IT1对恶性多形性腺瘤miR-195/CyclinD1的调控作用探讨[J]. 上海口腔医学, 2023, 32(1): 85-90.
[13]	潘巧婷, 臧晓龙, 孙照薇, 潘梦琪, 朱鑫美, 李志勇. 信号素4D在大鼠双膦酸盐相关颌骨坏死发生中的作用探讨[J]. 上海口腔医学, 2022, 31(6): 625-631.
[14]	朱江, 许腾, 宋冬惠, 吴森斌. 口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析[J]. 上海口腔医学, 2022, 31(5): 517-522.
[15]	黄纯, 温馨, 黄乐, 赵宁. 上颌前方牵引对恒牙早期骨性Ⅲ类错上、下颌骨宽度影响的三维模拟分析[J]. 上海口腔医学, 2022, 31(5): 550-555.