Ca(OH)2和Hoshino制剂对猪牙乳头细胞增殖的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (4): 419-422.

Ca(OH)₂和Hoshino制剂对猪牙乳头细胞增殖的影响

邱思慧, 汪俊

上海交通大学医学院附属第九人民医院·口腔医学院儿童口腔科,上海市口腔医学重点实验室,上海 200011

收稿日期:2014-10-20 出版日期:2015-08-20 发布日期:2015-09-10
作者简介:邱思慧（1988-）,女,硕士研究生,
基金资助:
上海市科学技术委员会医学引导项目（124119b0101）

Effects of calcium hydroxide and Hoshino’s paste on the proliferation of porcine dental papilla cells

QIU Si-hui, WANG Jun

Department of Pediatric Dentistry, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-10-20 Online:2015-08-20 Published:2015-09-10
Supported by:
Supported by Medical Guide Project of Science and Technology Committee of Shanghai Municipality (124119b0101）.

摘要/Abstract

摘要： 目的研究Ca(OH)₂和Hoshino制剂（甲硝唑、环丙沙星、米诺环素混合制剂）对体外培养的猪牙乳头细胞（procine dental papilla cells,pDPCs)增殖的影响,探讨临床使用牙髓血管再生治疗时根管消毒药物的选择。方法将第4代pDPCs分别在含不同浓度Ca(OH)₂（10%、1%、0.1%）和Hoshino制剂（0.5%、0.1%、0.05%）的培养基中培养,采用CCK-8法检测2种药物对细胞增殖活性的影响,采用SPSS19.0软件包对实验结果进行统计学分析。结果Ca(OH)₂对pDPCs细胞的增殖影响呈波动状,细胞培养9 d时,各组浓度Ca(OH)₂对细胞增殖无影响;Hoshino制剂可抑制pDPCs的增殖。结论实验中各浓度组Ca(OH)₂ 制剂对pDPCs 细胞的增殖无影响,而Hoshino制剂可抑制pDPCs 细胞的增殖。

关键词: Ca(OH)2制剂, Hoshino制剂, 猪牙乳头细胞

Abstract: PURPOSE: To observe the effects of calcium hydroxide and Hoshino’s paste(a mixture of metronidazole, ciprofloxacin, and minocycline) on the proliferation of porcine dental papilla cells (pDPCs), and to provide some theoretical basis for the choice of root canal disinfection drugs for pulp revascularization treatment. METHODS: The 4th generation of pDPCs were treated with several concentrations of (10%,1%,0.1%) and Hoshino’s paste (0.5%, 0.1%, 0.05%) medium for certain time periods. CCK-8 assay was performed to detect the effect of the 2 drugs on cell proliferation. SPSS19.0 software package was used for one-way ANOVA. RESULTS: The effect of Ca(OH)₂ on pDPCs proliferation was fluctuating, but cell proliferation of each Ca(OH)₂ group had no difference after 9 days. Meanwhile, cell proliferation was inhibited by Hoshino’s paste. CONCLUSIONS: Each concentration of in the study has no effect on pDPCs proliferation , while Hoshino’s paste can inhibit cell proliferation.

Key words: Calcium hydroxide, Hoshino’s paste, Procine dental papilla cells Shanghai J Stomatol,2015, ,24(4)419?422.

中图分类号:

R782.1

邱思慧, 汪俊. Ca(OH)₂和Hoshino制剂对猪牙乳头细胞增殖的影响[J]. 上海口腔医学, 2015, 24(4): 419-422.

QIU Si-hui, WANG Jun. Effects of calcium hydroxide and Hoshino’s paste on the proliferation of porcine dental papilla cells[J]. Shanghai Journal of Stomatology, 2015, 24(4): 419-422.

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Ca(OH)₂和Hoshino制剂对猪牙乳头细胞增殖的影响

Effects of calcium hydroxide and Hoshino’s paste on the proliferation of porcine dental papilla cells

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