口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析

doi:10.19439/j.sjos.2022.05.013

上海口腔医学 ›› 2022, Vol. 31 ›› Issue (5): 517-522.doi: 10.19439/j.sjos.2022.05.013

口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析

朱江¹, 许腾², 宋冬惠¹, 吴森斌¹

1.南通大学附属医院口腔科, 江苏南通 226000;
2.南京医科大学附属口腔医院口腔颌面外科,江苏南京 210005

收稿日期:2021-07-29 修回日期:2021-09-27 出版日期:2022-10-25 发布日期:2022-11-01
通讯作者: 朱江,E-mail：zhujiang15910@163.com
作者简介:朱江（1988-）,男,硕士,主治医师
基金资助:
江苏省自然科学基金（2019489）; 南通市科技局课题（JCZ19112）

Study on the correlation between combined detection of RACK1, M2/M1in oral squamous cell carcinoma, prognosis of the patients

ZHU Jiang¹, XU Teng², SONG Dong-hui¹, WU Sen-bin¹

1. Department of Stomatology, Affiliated Hospital of Nantong University. Nantong 226000;
2. Department of Oral and Maxillofacial Surgery, Stomatological College of Nanjing Medical University. Nanjing 210005, Jiangsu Province, China

Received:2021-07-29 Revised:2021-09-27 Online:2022-10-25 Published:2022-11-01

摘要/Abstract

摘要： 目的: 探讨口腔鳞状细胞癌（OSCC）组织中活性蛋白激酶C受体1（RACK1）与M2和M1型巨噬细胞的比值（M2/M1）联合检测与预后的关系。方法: 选择2017年2月—2018年5月南通大学附属医院收治的OSCC患者129例,检测并对比癌组织、癌旁组织中RACK1及M2/M1情况,分析癌组织中RACK1表达、M2/M1与临床病理因素及预后的关系。采用SPSS 18.0软件包对数据进行统计学分析。结果: 癌组织中RACK1阳性表达率、M2/M1值显著高于癌旁组织（P<0.05）。Ⅲ~Ⅳ期、有淋巴结转移、累及脉管患者癌组织中RACK1阳性表达率分别高于Ⅰ~Ⅱ期、无淋巴结转移、无累及脉管患者（P<0.05）,Ⅲ~Ⅳ期、低分化、有淋巴结转移患者癌组织中M2/M1值显著高于Ⅰ~Ⅱ期、中高分化、无淋巴结转移患者（P<0.05）。Cox回归分析显示,肿瘤分期、淋巴结转移、RACK1阳性表达率、M2/M1是影响总生存率的独立预后因素（P<0.05）。ROC曲线显示,癌组织RACK1、M2/M1及两者联合预测OSCC患者预后的AUC分别为0.743、0.718、0.875。RACK1阳性表达患者生存率为62.24%,RACK1阴性表达患者生存率为92.31%;RACK1阳性与阴性表达患者的总生存率曲线相比,差异有统计学意义（P<0.05）。M2/M1≥2.06患者的存活率为61.70%,M2/M1<2.06患者的生存率为88.24%;M2/M1≥2.06与M2/M1<2.06患者的总生存率曲线相比,差异有统计学意义（P<0.05）。结论: OSCC患者癌组织中RACK1、M2/M1异常高表达,并与患者病理情况和预后相关,两者联合预测患者预后具有一定效能。

关键词: 口腔, 鳞状细胞癌, 活性蛋白激酶C受体1, M2型巨噬细胞, M1型巨噬细胞, 预后

Abstract: PURPOSE: To explore the relationship between combined detection of tissue active protein kinase C receptor 1 (RACK1) and M2/M1 and the prognosis of oral squamous cell carcinoma (OSCC). METHODS: 129 OSCC patients admitted to the Affiliated Hospital of Nantong University were selected as the research subjects from February 2017 to May 2018. RACK1 and M2/M1 in cancer tissues and adjacent tissues of patients were detected and compared. The relationship between RACK1 expression, M2/M1 and clinicopathological factors, and relationship between RACK1 expression, M2/M1 and prognosis in cancer tissue were analyzed. SPSS 18.0 software package was used for data analysis. RESULTS: The positive expression rate of RACK1 and the value of M2/M1 in cancer tissues were significantly higher than those in adjacent tissues (P<0.05). The positive expression rate of RACK1 in cancer tissues of patients with stage Ⅲ-Ⅳ, cervical lymph node metastasis, and vascular involvement were significantly higher than those with stage Ⅰ-Ⅱ, no lymph node metastasis, and no vascular involvement(P<0.05). M2/M1 values in cancer tissues of patients with stage Ⅲ-Ⅳ, poor differentiation, and cervical lymph node metastasis were significantly higher than those with stage Ⅰ-Ⅱ, moderate differentiation, and no cervical lymph node metastasis(P<0.05). Cox regression analysis showed that the stage of tumor, cervical lymph node metastasis, positive expression rate of RACK1, and M2/M1 were independent prognostic factors affecting overall survival (P<0.05). ROC curve showed that AUC of cancer tissue RACK1, M2/M1 and combination of the two to predict the prognosis of OSCC patients were 0.743, 0.718 and 0.875, respectively. The survival rate of patients with positive expression of RACK1 was 62.24%, and the survival rate of patients with negative expression of RACK1 was 92.31%. The overall survival rate of patients with positive expression of RACK1 and patients with negative expression were compared, and the difference was statistically significant(P<0.05). The survival rate of patients with M2/M1≥2.06 was 61.70%, the survival rate of patients with M2/M1<2.06 was 88.24%, and the overall survival curve of patients with M2/M1≥2.06 was compared with that of patients with M2/M1<2.06, and the difference was statistically significant(P<0.05). CONCLUSIONS: The expression of RACK1 and M2/M1 is abnormally high in cancer tissues of OSCC patients. The expression of RACK1 and M2/M1 is related to the patient's pathology and prognosis. The combination of the two has certain efficacy in predicting the prognosis of OSCC patients.

Key words: Oral cavity, Squamous cell carcinoma, Active protein kinase C receptor 1, M2 type macrophages, M1 type macrophages, Prognosis

中图分类号:

R739.8

朱江, 许腾, 宋冬惠, 吴森斌. 口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析[J]. 上海口腔医学, 2022, 31(5): 517-522.

ZHU Jiang, XU Teng, SONG Dong-hui, WU Sen-bin. Study on the correlation between combined detection of RACK1, M2/M1in oral squamous cell carcinoma, prognosis of the patients[J]. Shanghai Journal of Stomatology, 2022, 31(5): 517-522.

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口腔鳞状细胞癌组织中RACK1与M2/M1联合检测与预后的相关性分析

Study on the correlation between combined detection of RACK1, M2/M1in oral squamous cell carcinoma, prognosis of the patients

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