钛表面微沟槽形貌对人牙龈纤维细胞黏着斑蛋白表达的影响

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (2): 182-187.

钛表面微沟槽形貌对人牙龈纤维细胞黏着斑蛋白表达的影响

赖颖真¹,陈江²,林珊³

1.厦门医学高等专科学校口腔医学系,福建厦门 361000;
2.福建医科大学附属口腔医院种植科,福建福州 350002;
3.福建医科大学附属第一医院口腔科,福建福州 350000

收稿日期:2014-07-25 出版日期:2015-04-20 发布日期:2015-07-24
通讯作者: 陈江,Tel：0591-83735488,E-mail:121708065@qq.com
作者简介:赖颖真（1984- ）,女,博士研究生,讲师、主治医师,E-mail:dentistyz@126.com

Effects of morphology of the microgroove titanium surface on expression of vinculin in human gingival fibroblasts

LAI Ying-zhen¹,CHEN Jiang²,LIN Shan³

1.Department of Oral Medicine, Xiamen Medical College. Xiamen 361000;
2. Department of Oral Implantology, Affiliated Stomatological Hospital of Fujian Medical University. Fuzhou 350002;
3. Department of Stomatology, First Affiliated Hospital of Fujian Medical University. Fuzhou 350000, Fujian Province,China

Received:2014-07-25 Online:2015-04-20 Published:2015-07-24

摘要/Abstract

摘要： 目的比较不同尺寸微沟槽表面对人牙龈成纤维细胞（HGF）黏着斑蛋白vinculin表达的影响,作为选择种植体穿龈部分微沟槽尺寸的依据之一。方法针对HGF大小,设计不同沟槽尺寸,利用光刻技术制作微沟槽形貌,沟槽宽度和间隔分别为15 μm、30 μm和60 μm,沟槽深度为5 μm和10 μm,分组为T15/5、T15/10、T30/5、T30/10、T60/5和T60/10,光滑钛(T0)表面为对照组。通过免疫荧光、实时荧光定量PCR和蛋白免疫印迹比较vinculin基因和蛋白水平的表达差异。采用SPSS17.0软件包对数据进行统计学分析。结果免疫荧光显示,随着沟槽宽度增加,vinculin 的绿色荧光点增多,mRNA和蛋白水平表达T60最高,T15最低。相同沟槽宽度下,深度改变对该蛋白的表达无显著影响。结论微沟槽形貌有利于vinculin蛋白的表达,表达量随着沟槽的宽度增加而增加。就vinculin蛋白的表达而言,T60组沟槽更适合作为穿龈部分的沟槽尺寸。

关键词: 种植体, 微沟槽, 人牙龈成纤维细胞, 黏着斑蛋白

Abstract: PURPOSE: To investigate the effects of morphology of the microgroove surface on the expression of vinculin in human gingival fibroblasts, with the hope of providing basis for size selection of microgroove for transmucosal part of dental implants. METHODS: Microgroove titanium surfaces were fabricated by photolithography with parallel grooves: 15 μm, 30 μm or 60 μm in width and 5 μm or 10 μm in depth. The groups that used different microgroove surfaces were denoted as T15/5, T15/10, T30/5, T30/10, T60/5, and T60/10. Smooth titanium surfaces (T0) were used as controls. Surface topography was detected. HGFs were cultured on the microgroove surfaces. Morphology of vinculin was analyzed using CLSM. Expression level of vinculin was compared among different groups by real-time PCR and Western blotting. SPSS17.0 software package was used for statistical analysis. RESTULTS: Immunofluorescence staining revealed increase of green vinculin fluorescent spots as the increase of microgroove width, while T60 group had the highest mRNA and protein expression and T15 group had the lowest. Microgroove depth had no effect on the expression of vinculin under the same width. CONCLUSIONS: Morphology of microgroove surface has effect on the expression of vinculin, and the morphology of microgroove surface in T60 group is the best size for the transmucosal part in terms of expression of vinculin protein expression.

Key words: Dental implant, Microgroove, Human gingival fibroblasts, Vinculin

中图分类号:

R782，R783.1

赖颖真,陈江,林珊. 钛表面微沟槽形貌对人牙龈纤维细胞黏着斑蛋白表达的影响[J]. 上海口腔医学, 2015, 24(2): 182-187.

LAI Ying-zhen,CHEN Jiang,LIN Shan. Effects of morphology of the microgroove titanium surface on expression of vinculin in human gingival fibroblasts[J]. Shanghai Journal of Stomatology, 2015, 24(2): 182-187.

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