表皮生长因子与干燥综合征伴发萎缩性舌炎的相关性研究

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (6): 731-735.

表皮生长因子与干燥综合征伴发萎缩性舌炎的相关性研究

袁昌青^{1*, *}, 曹颖秀^{2*, *}, 刘斌³, 董静³, 邓婧¹, 耿国梁¹

1.青岛大学附属医院口腔科,山东青岛 266003; 2.德州市人民医院口腔科,山东德州 253014; 3.青岛大学附属医院风湿免疫科,山东青岛 266003

收稿日期:2013-11-27 修回日期:2014-03-04 出版日期:2014-12-20 发布日期:2015-01-08
通讯作者: 袁昌青,Tel:0532-82912065,E-mail:ycq613@163.com E-mail:caoyx0619@163.com
作者简介:袁昌青（1970-）,女,硕士,副主任医师,硕士研究生导师;曹颖秀（1989-）,女,硕士研究生。*并列第一作者

Study on the correlation of epidermal growth factor and Sjögren’s syndrome with atrophic glossitis

YUAN Chang-qing, CAO Ying-xiu, LIU Bin, DONG jing, DENG Jing, GENG Guo-liang

1.Department of Stomatology,the Affiliated Hospital of Qingdao University.Qingdao 266033;
2.Department of Stomatology, Dezhou People’s Hospital, Dezhou 253014;
3.Department of Rheumatology, the Affiliated Hospital of Qingdao University. Qingdao 266003, Shandong Province, China

Received:2013-11-27 Revised:2014-03-04 Online:2014-12-20 Published:2015-01-08

摘要/Abstract

摘要： 目的：探讨表皮生长因子(epidermal growth factor,EGF)及其受体（EGF receptor,EGFR)与干燥综合征（Sjögren’s syndromes,SS)伴发萎缩性舌炎（atrophic glossitis,AG)的相关性及可能的作用机制。方法：干燥综合征患者按未伴发与伴发轻、中、重度萎缩性舌炎分为4组,健康成年人为对照组,应用ELISA法检测唾液中的EGF含量,SP免疫组化技术检测舌上皮细胞中EGFR的表达情况,采用SPSS19.0软件包进行统计学处理。结果：干燥综合征未伴发及伴发萎缩性舌炎组唾液EGF浓度均显著低于正常对照组(P<0.01),2组之间差异显著（P=0.024）,干燥综合征伴发轻、中、重萎缩性舌炎组唾液EGF水平逐渐降低,两两比较差异显著（P<0.05）;干燥综合征伴发中、重度萎缩性舌炎组舌上皮细胞EGFR表达较正常对照组显著降低（P=0.009,P=0.037）,其余2组与对照组之间差异无显著性;舌背黏膜萎缩程度与唾液EGF水平显著相关（r=-0.673,P<0.01）。结论：干燥综合征患者唾液中表皮生长因子浓度明显降低,且与其伴发萎缩性舌炎有密切相关性。

关键词: 干燥综合征, 萎缩性舌炎, 表皮生长因子, 表皮生长因子受体

Abstract: PURPOSE: To investigate the correlation between epidermal growth factor (EFG) and atrophic glossitis (AG) in patients with Sjögren’s syndromes (SS) and explore its pathogenesis. METHODS: Ninety-three patients with SS (60 with AG and 33 without AG) and 20 normal were selected. The concentrations of EGF in saliva were analyzed by ELISA. The expressions of EGF receptor (EGFR) in the epithelial cells of the tongue were assayed by immunohistochemistry. The differences among each group were analyzed with SPSS19.0 software package. RESULTS: The saliva EGF concentrations in SS was lower than that in normal control group(P<0.0001),and EGF concentrations in SS with AG was significantly lower than that in SS without AG (P=0.024). EGF levels in saliva gradually decreased in the mild, moderate and severe atrophic glossitis groups, and there were significant differences among each group(P<0.05). EGFR in the epithelial cells of tongue was lower in SS with moderate and severe AG than in the control group(P=0.009, P=0.037), and there was a significant correlation between EGF and the degree of AG (r=-0.673, P<0.01). CONCLUSIONS: Saliva EGF concentrations decrease significantly in patients with SS and it is closely related to the morbidity of atrophic glossitis.

Key words: Sjögren’s syndrome, Atrophic glossitis, Epidermal growth factor, Epidermal growth factor receptor

中图分类号:

R781.6

袁昌青, 曹颖秀, 刘斌, 董静, 邓婧, 耿国梁. 表皮生长因子与干燥综合征伴发萎缩性舌炎的相关性研究[J]. 上海口腔医学, 2014, 23(6): 731-735.

YUAN Chang-qing¹, CAO Ying-xiu², LIU Bin³, DONG jing³, DENG Jing¹, GENG Guo-liang¹. Study on the correlation of epidermal growth factor and Sjögren’s syndrome with atrophic glossitis[J]. Shanghai Journal of Stomatology, 2014, 23(6): 731-735.

参考文献

[1] 崔志新, 崔彦博, 唐燕红, 等. 表皮生长因子对表皮细胞增殖和凋亡的调控作用 [J]. 食品与药品, 2005, 7(11): 15-19.
[2] Epstein JB, Gorsky M, Guglietta A, et a1. The correlation between epidermal growth factor levels in saliva and the severity of oral mucositis during oropharyngeal radiation therapy[J]. Cancer, 2000, 89(11): 2258-2265.
[3] Vitali C, Bombardieri S, Jonsson R, et a1. Classification criteria for Sjögren’s syndrome: a revised version of the European criteria proposed by the American-European Consensus Group[J]. Ann Rheum Dis, 2002, 61(6): 554-558.
[4] 朱敬慈, 周永梅, 沈雪敏, 等. 应用数码显微镜评价重组牛碱性成纤维细胞生长因子辅助治疗萎缩性舌炎的临床效果[J]. 临床口腔医学杂志, 2012, 28(1): 25-28.
[5] 戴豪良. 舌诊研究与临床应用[M]. 上海: 上海科学技术出版社, 2006: 134-135, 158-160.
[6] Laurent M, Gogly B, Tahmasebi F, et al. Oropharyngeal candidiasis in elderly patients [J]. Geritr Psychol Neuropsychiatr Vieil, 2011, 9(1): 21-28.
[7] Maeda M. Dermoscopic patterns of the filiform papillae of the tongue in patient with a Sjögren’s syndrome[J]. J Dermatol, 2006, 33(2): 96-102.
[8] 刘岩, 王献华, 周玮玮. 表皮生长因子与器官纤维化 [J]. 现代预防医学, 2008, 35(7): 1398-1400.
[9] Routsias JG, Tzioufas AG. Autoimmune response and target autoantigens in Sjögren’s syndrome[J]. Eur J Clin Invest, 2010, 40(11): 1026-1036.
[10] Szodoray P, Gal I, Barath S, et al. Immunological alterations in newly diagnosed primary Sjögren’s syndrome characterized by skewed peripheral T-cell subsets and inflammatory cytokines [J]. Scand J Rheumatol, 2008, 37(3): 205-212.
[11] Pijpe J, Kalk WW, Bootsma H, et al. Progression of salivary gland dysfunctionn in patients with Sjögren’s syndrome [J]. Ann Rheum Dis, 2007, 66(1): 107-112.
[12] Shilo BZ. Regulating the dynamics of EGF receptor signaling in space and time [J]. Development, 2005, 132(18): 4017-4027.
[13] 佟书娟, 张军峰, 詹瑧. 舌苔形成与CD54和EGF-R表达关系的初步探讨[J]. 南京中医药大学学报(自然科学版),2010,26(2): 118-119.
[14] Konturek PC, Brzozowski T, Duda A, et aI. Epidermal growth factor and prostaglandin E(2)accelerate mucosal recovery from stress-induced gastric lesions via inhibition of apoptosis [J]. J Physiol Paris, 2001, 95(1-6): 361-367.
[15] Leu CM, Chang C, Hu C. Epidermal growth factor (EGF) suppresses staurosporine-induced apoptosis by inducing mcl-1 via the mitogen-activated protein kinase pathway [J].Oncogene, 2000, 19(13): 1665-1675.
[16] Kennedy NJ, Cellurale C, Davis RJ. A radical role for p38 MAPK in tumor initiation [J]. Cancer Cell, 2007, 11(2): 101-103.
[17] Dunn KL, Espino PS, Drobic B, et al. The Ras-MAPK signal transduction pathway, cancer and chromatin remodeling [J]. Biochem Cell Biol, 2005, 83(1): l-14.
[18] 张军峰, 范媛, 詹臻, 等. 舌背黏膜上皮细胞的凋亡和周期变化探索研究 [J]. 口腔医学研究, 2009, 25(1): 38-40.