上海口腔医学

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (2): 168-173.doi: 10.19439/j.sjos.2020.02.011

• 论著 • 上一篇    下一篇

载药脂质体-微泡复合物超声导向治疗头颈鳞癌的体外实验研究

周璐, 龚霞, 熊屏   

  1. 上海交通大学医学院附属第九人民医院 超声科,上海 200011
  • 收稿日期:2019-06-11 修回日期:2019-10-10 出版日期:2020-04-25 发布日期:2020-04-30
  • 通讯作者: 熊屏,E-mail:xiong_ping_xp@163.com
  • 作者简介:周璐(1992-),女,硕士,E-mail:818168@sh9hospital.org
  • 基金资助:
    上海交通大学医工交叉基金(YG2016QN14)

Gefitinib/chloroquine-liposome-microbubble complexes as ultrasound-triggered therapeutic particles for head and neck squamous cell carcinoma in vitro

ZHOU Lu, GONG Xia, XIONG Ping   

  1. Department of Ultrasound, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University of Medicine. Shanghai 200011, China
  • Received:2019-06-11 Revised:2019-10-10 Online:2020-04-25 Published:2020-04-30

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摘要： 目的 体外研究一种具有靶向性、副作用少、同时具备诊断和治疗作用的针对头颈鳞癌治疗的微泡颗粒。方法 分别制备载吉非替尼脂质体-微泡模型和载氯喹脂质体-微泡模型。将HN-30细胞分为5组,即PBS缓冲液组、载吉非替尼脂质体+载氯喹脂质体(GE-Lipo+CQ-Lipo)组、载吉非替尼脂质体-微泡模型(MB-GE-Lipo)组、载氯喹脂质体-微泡模型(MB-CQ-Lipo)组和载吉非替尼脂质体-微泡模型+载氯喹脂质体-微泡模型(MB-GE-Lipo+ MB-CQ-Lipo)组。检测各组细胞的增殖抑制效果。采用SPSS 17.0软件包对数据进行统计学分析。结果 当卵磷脂酰胆碱(egg phosphatidylcholine)∶胆固醇(Cholesterol)=2∶1时,与8∶1组相比,包封率无显著差异(P>0.05),2∶1与5∶1、10∶1、20∶1组相比,差异有统计学意义(P<0.05);5∶1、8∶1、10∶1、20∶1 4组之间两两比较,无显著差异(P>0.05)。对于HN-30细胞的增殖抑制作用,(MB-GE-Lipo+ MB-CQ-Lipo)组与PBS组相比,差异有统计学意义(P<0.05);(MB-GE-Lipo+ MB-CQ-Lipo)组与(MB-GE-Lipo)组相比,差异无统计学意义(P>0.05)。结论 当EPC∶Chol=5∶1时,载吉非替尼脂质体包封率较高,且胆固醇比例合适,脂质体较稳定。MB-GE-Lipo+ MB-CQ-Lipo联合超声对HN-30细胞的增殖抑制作用明显。

关键词: 超声微泡, 靶向, 表皮生长因子受体, 头颈鳞癌

Abstract: PURPOSE: To explore a kind of therapeutic particle of targeted, fewer side effects, both with value of diagnosis and treatment for head and neck squamous cell carcinoma(HNSCC) in vitro. METHODS: Gefitinib-liposome-microbubble model and chloroquine liposome-microbubble model were prepared separately. HN-30 cells were divided into 5 groups: PBS, GE-Lipo+CQ-Lipo, MB-GE-Lipo, MB-CQ-Lipo, MB-GE-Lipo+ MB-CQ-Lipo. MTS kit was used to detect the inhibitory effect of cell proliferation in each group. SPSS17.0 software package was used to analyze the data. RESULTS: When EPC: Chol=2:1, there was no significant difference in the entrapment efficiency between 2:1 and 8:1 group (P>0.05). Compared with 5:1,10:1,20:1 group, there was significant difference in the entrapment efficiency of 2:1 group (P<0.05). Among group 5:1, 8:1, 10:1, and 20:1, compared with each other, there was no significant difference (P>0.05). For HN-30 cells, there was significant difference(P<0.05) between MB-GE-Lipo+ MB-CQ-Lipo group and PBS group. There was no significant difference between MB-GE-Lipo+ MB-CQ-Lipo group and MB-GE-Lipo group (P>0.05). CONCLUSIONS: When EPC:Chol=5:1, the EE of GE-Lipo was higher, the ratio of cholesterol was appropriate, and the liposome was more stable. The inhibitory effect of gefitinib liposome-microbubbles + chloroquine liposome-microbubbles combined with ultrasound on HN-30 cell proliferation was significant.

Key words: Microbubble, Targeting, EGFR, Head and neck squamous cell carcinoma

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