饥饿状态对白念菌生物膜及滞留菌形成的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (3): 285-289.

饥饿状态对白念菌生物膜及滞留菌形成的影响

武侠, 孙静, 褚昊月, 亓庆国

山东大学口腔医学院牙体牙髓病科,山东省口腔生物医学重点实验室,山东济南 250012

收稿日期:2013-12-27 出版日期:2014-06-20 发布日期:2014-09-09
通讯作者: 亓庆国,Tel:0531-88381085,E-mail:qqg@sdu.edu.cn
作者简介:武侠(1989-), 女, 硕士研究生, E-mail:wuxia5110@163.com
基金资助:
国家自然科学基金(30973310, 81371158)

The influence of starvation on Candida albicans biofilm and persisters formation

WU Xia, SUN Jing, CHU Hao-yue, QI Qing-guo

Department of Endodontics, School of Stomatology, Shandong University, Key Lab of Oral Biomedicine of Shandong Province. Jinan 250012, Shandong Province, China

Received:2013-12-27 Online:2014-06-20 Published:2014-09-09
Supported by:
Supported by National Natural Science Foundation of China (30973310, 81371158).

摘要/Abstract

摘要： 目的:探讨饥饿环境对白念菌生物膜形成以及其中耐药滞留菌形成的影响。方法:体外分别构建对数期、稳定期和饥饿态白念菌24 h生物膜,两性霉素B(Amphotericin B)冲击24 h,冲击前、后行活菌菌落计数(CFU),计算得出滞留菌比例并进行统计学分析,同时以共聚焦显微镜观察和分析各组生物膜形成状况。采用SPSS11.70软件包对数据进行统计学分析。结果:对数期、稳定期和饥饿态白念菌生物膜形成能力依次下降 (P<0.05),具有显著差异,前2组形成菌丝相生物膜,而饥饿态菌形成酵母相生物膜。药物作用后,滞留菌比例依次升高(P<0.05),具有显著差异。结论:营养状态对白念菌滞留菌的产生有影响,饥饿状态能够提高滞留菌的产生比例。

关键词: 白念菌, 生物膜, 滞留菌, 饥饿

Abstract: PURPOSE: To investigate the influence of starvation on Candida albicans(C. albicans) biofilm and persisters formation. METHODS: The growth curve was made by CFU counts methods in different culture times to find the exponential and stationary phases. C. albicans in exponential, stationary and starvation phases were developed respectively in 96-well microtiter plates to form 24 h biofilms model. Then the biofilms before and after amphotericin B (100 ?g/ml) treatment were quantified to determine the number of live cells. Statistical analysis was performed using SPSS17.0 software package. The biofilm structure was observed by confocal scanning laser microscopy. RESULTS: Biofilm formation capacity of C. albicans in exponential, stationary and starvation phases reduced successively (P<0.05). The biofilms formed by starved C. albicans were predominately composed of yeast cells, compared with cells in exponential and stationary biofilms, which displayed filamentous growth. The ratios of persisters increased successively (P<0.05). CONCLUSIONS: The formation of C. albicans persisters can be influenced by environment events, and starvation can increase the percentage of persister cells.

Key words: Candida albicans, Biofilm, Persister cells, Starvation

中图分类号:

R780.2

武侠, 孙静, 褚昊月, 亓庆国. 饥饿状态对白念菌生物膜及滞留菌形成的影响[J]. 上海口腔医学, 2014, 23(3): 285-289.

WU Xia, SUN Jing, CHU Hao-yue, QI Qing-guo. The influence of starvation on Candida albicans biofilm and persisters formation[J]. Shanghai Journal of Stomatology, 2014, 23(3): 285-289.

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