柚皮苷对犬骨髓基质细胞体外增殖及成骨分化的影响

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (3): 280-284.

柚皮苷对犬骨髓基质细胞体外增殖及成骨分化的影响

张学斌^{1, 2}, 刘天麟², 张忻¹, 谭鸾君², 葛海良³, 黄远亮²

1.同济大学口腔医学院口腔生物医学及转化医学实验室,上海 200072;
2.同济大学附属东方医院口腔科,上海 200120;
3.上海交通大学医学院免疫学教研室,上海 200025

收稿日期:2013-10-14 出版日期:2014-06-20 发布日期:2014-09-09
通讯作者: 黄远亮,E-mail: ylhuang0115@163.com
作者简介:张学斌(1985-), 男, 在读硕士研究生, E-mail: 2011zzh@tongji.edu.cn
基金资助:
国家自然科学基金(81070806); 上海市科学技术委员会资助项目(10JC1413300); 浦东新区卫生局青年项目(PW2011B-13)

Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro

ZHANG Xue-bin^1,2, LIU Tian-lin², ZHANG Xin¹, TAN Ruan-jun², GE Hai-liang³, HUANG Yuan-liang²

1.Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University. Shanghai 200072;
2.Department of Stomatology, the Affiliated East Hospital of Tongji University.Shanghai 200120;
3.Department of Immunology, Shanghai Jiao Tong University School of Medicine. Shanghai 200025, China

Received:2013-10-14 Online:2014-06-20 Published:2014-09-09
Supported by:
Supported by National Natural Science Foundation of China (81070806), Research Fund of Science and Technology Committee of Shanghai Municipality (10JC1413300) and Youth Foundation of Pudong Municipal Commission of Health and Family Planning (PW2011B-13).

摘要/Abstract

摘要： 目的:研究犬骨髓基质细胞(bone marrow stromal cells, BMSCs)在柚皮苷诱导下定向成骨及成骨分化能力。方法:抽取犬骨髓,贴壁法体外培养,流式细胞仪鉴定,加入不同浓度柚皮苷(1×10-5、1×10-6、1×10-7、1×10-8、1×10-9 mol/L)诱导,以CCK-8检测药物毒性,定量检测碱性磷酸酶、von Kossa检测钙结节形成。采用SPSS20.0软件包对数据进行统计学分析。结果:经流式细胞仪检测,CD34、CD45低表达、CD90高表达分别为0.126%、0.075%和95.4%;柚皮苷浓度在10-6、10-7 mol/L时,能明显促进细胞增殖;经柚皮苷诱导后,碱性磷酸酶含量显著提高,其中,浓度为10-6 mol/L时,ALP相对值最高(P<0.05);von Kossa钙结节检测呈阳性。结论:犬骨髓基质细胞在柚皮苷诱导下能分化为成骨细胞。柚皮苷浓度为10-6 mol/L时,能明显促进骨髓基质细胞的增殖及分化。

关键词: 柚皮苷, 骨髓基质细胞, 成骨诱导, 骨组织工程

Abstract: PURPOSE: To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells (BMSCs) in dogs. METHODS:BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin (1×10-5, 1×10-6, 1×10-7, 1×10-8 and 1×10-9 mol/L) were added to cell culture media to induce BMSCs. The effect of Naringin on BMSCs was evaluated respectively by CCK-8 method and measuring the activity of alkaline phosphatase (ALP). The formation of nodules of calcium was detected by von Kossa staining. The data was analyzed with SPSS20.0 software package. RESULTS: The result of cell-surface marker displayed that the expression of CD34 and CD45 were negative while the expression of CD90 was positive. The values were 0.126%, 0.075% and 95.4%, respectively. Naringin could obviously promote cell proliferation, which exhibited the best effect on proliferation and osteogenic differentiation at concentration of 10-6 mol/L. Calcium nodule (von Kossa) staining was positive. CONCLUSIONS: Naringin-induced bone marrow stromal cells can be differentiated into osteoblasts. Naringin at the concentration of 10-6 mol/L can enhance the proliferation and osteogenic differentiation of BMSCs.

Key words: Naringin, BMSCs, Osteoinduction, Bone tissue engineering

中图分类号:

R782

张学斌, 刘天麟, 张忻, 谭鸾君, 葛海良, 黄远亮. 柚皮苷对犬骨髓基质细胞体外增殖及成骨分化的影响[J]. 上海口腔医学, 2014, 23(3): 280-284.

ZHANG Xue-bin, LIU Tian-lin, ZHANG Xin, TAN Ruan-jun, GE Hai-liang, HUANG Yuan-liang. Effect of Naringin on proliferation and osteogenic differentiation of bone marrow stromal cells in vitro[J]. Shanghai Journal of Stomatology, 2014, 23(3): 280-284.

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