FSP改性TiNbTaZr/Zn的表征和成骨诱导活性评价

doi:10.19439/j.sjos.2021.05.005

上海口腔医学 ›› 2021, Vol. 30 ›› Issue (5): 472-476.doi: 10.19439/j.sjos.2021.05.005

FSP改性TiNbTaZr/Zn的表征和成骨诱导活性评价

严毅^1,2, 杨智¹, 王立强³, 傅远飞¹

1.上海交通大学医学院附属第九人民医院口腔修复科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海 200011;
2.上海市黄浦区香山中医医院口腔科,上海 200020;
3.上海交通大学材料学院与工程学院,金属基复合材料国家重点实验室,上海 200240

收稿日期:2021-03-01 修回日期:2021-04-16 出版日期:2021-10-25 发布日期:2021-11-08
通讯作者: 傅远飞,E-mail：fuyuanf@163.com
作者简介:严毅（1974-）,女,学士,主治医师,E-mail：firstdooryy@aliyun.com
基金资助:
国家自然科学基金（31971246）

Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn

YAN Yi^1,2, YANG Zhi¹, WANG Li-qiang³, FU Yuan-fei¹

1. Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
2. Department of Stomatology, Xiangshan Hospital of Traditional Chinese Medicine, Huangpu District. Shanghai 200020;
3. State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University. Shanghai 200240, China

Received:2021-03-01 Revised:2021-04-16 Online:2021-10-25 Published:2021-11-08

摘要/Abstract

摘要： 目的: 探讨搅拌摩擦处理加工后的Ti-35Nb-2Ta-3Zr/ Zn（TNTZ/ Zn）复合材料是否具备诱导成骨的生物活性。方法: 通过搅拌摩擦处理,将Zn添加到TNTZ合金表面,设计对照组为TNTZ（未经FSP的TNTZ）,实验组为FSP（未加Zn加工的TNTZ）、TNTZ/Zn-0.5（0.5 mm预制孔）和TNTZ/Zn-1（1 mm预制孔）。使用扫描电子显微镜、X线衍射进行表征分析,检测共培养的大鼠骨髓基质干细胞（bone marrow stromal stem cells,BMSCs）的碱性磷酸酶（ALP）活性,实时定量 PCR 检测ALP活性,COL-1α、OPN和OCN基因的表达。采用 SAS 8.2软件包对数据进行统计学分析。结果: TNTZ/Zn-1组表面Zn纳米粒子分布较均匀,直径为70~80 nm。与TNTZ组相比,TNTZ/Zn-0.5和TNTZ/Zn-1组的ALP表达显著上调（P<0.05和 P<0.01）。相比于TNTZ组,TNTZ/Zn-0.5和TNTZ/Zn-1组的ALP活性、COL-1α、OPN和OCN基因表达增加,差异有统计学意义（P<0.05）。结论: 通过FSP成功将Zn整合到TNTZ合金表面,制备出纳米级微观结构。TNTZ/Zn复合材料可有效诱导成骨,是一种潜在的种植体材料。

关键词: 骨髓基质干细胞, 成骨诱导活性, 搅拌摩擦处理

Abstract: PURPOSE: To investigate whether Ti-35Nb-2Ta-3Zr/Zn (TNTZ/Zn) composite material processed by friction stir processing has osteogenic activity. METHODS: Zn was added to the surface of TNTZ alloy by friction stir processing. The control group was TNTZ(TNTZ without FSP), the experimental groups was FSP(FSP without Zn), TNTZ/Zn-0.5 (0.5 mm pre-made holes) and TNTZ/Zn-1(1 mm pre-made holes). Surface characterization was performed by scanning electron microscope and X-ray diffraction. Alkaline phosphatase(ALP) activity of co-cultured rat bone marrow stromal stem cells (BMSCs) and COL-1α, OPN and OCN gene expression were detected by real-time quantitative PCR. SAS version 8.2 software was used for data analysis. RESULTS: Zn nanoparticles on the surface of the TNTZ/Zn-1 group were distributed homogeneously, with a diameter range of 70-80 nm. Compared with TNTZ group, the activity of ALP in TNTZ/Zn-0.5 and TNTZ/Zn-1 groups was up-regulated (P<0.05 and P<0.01). Compared with TNTZ group, ALP, COL-1α, OPN and OCN gene expression increased in TNTZ/Zn-0.5 and TNTZ/Zn-1 group, and the difference was statistically significant (P<0.05). CONCLUSIONS: Through FSP, Zn can be successfully integrated onto the surface of TNTZ alloy with nano-scale microstructures. TNTZ/Zn composite material can effectively induce bone formation and is a potential implant material.

Key words: Bone marrow stromal stem cells, Osteogenic activity, Friction stir processing

中图分类号:

R782.1

严毅, 杨智, 王立强, 傅远飞. FSP改性TiNbTaZr/Zn的表征和成骨诱导活性评价[J]. 上海口腔医学, 2021, 30(5): 472-476.

YAN Yi, YANG Zhi, WANG Li-qiang, FU Yuan-fei. Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn[J]. Shanghai Journal of Stomatology, 2021, 30(5): 472-476.

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FSP改性TiNbTaZr/Zn的表征和成骨诱导活性评价

Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn

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