牙源性间充质干细胞对成牙骨质细胞生物学特性的影响

doi:10.19439/j.sjos.2025.04.003

上海口腔医学 ›› 2025, Vol. 34 ›› Issue (4): 354-361.doi: 10.19439/j.sjos.2025.04.003

牙源性间充质干细胞对成牙骨质细胞生物学特性的影响

李雯雯¹, 马文盛², 李颖辉¹, 赵圆¹, 李东娜¹, 翟浩嫣¹, 刘春艳¹

1.河北医科大学口腔医学院·口腔医院正畸科, 河北省口腔医学重点实验室, 河北省口腔疾病临床医学研究中心, 河北石家庄 050017;
2.天津市口腔医院·南开大学口腔医院, 天津 300041

收稿日期:2024-04-22 修回日期:2024-07-12 出版日期:2025-08-25 发布日期:2025-08-26
通讯作者: 刘春艳,E-mail: chunyanliu@hebmu.edu.cn
作者简介:李雯雯（1998-）,女,硕士,住院医师,E-mail: liwenwen1218@sina.com
基金资助:
河北省重点研发计划项目（21377715D）;河北省科学技术厅2021年政府资助省级医学优秀人才项目（361029）

Effect of dental-derived stem cells on the biological characteristics of cementoblasts

Li Wenwen¹, Ma Wensheng², Li Yinghui¹, Zhao Yuan¹, Li Dongna¹, Zhai Haoyan¹, Liu Chunyan¹

1. Department of Orthodontics, School and Hospital of Stomatology, Hebei Medical University; Hebei Key Laboratory of Stomatology; Hebei Clinical Research Center for Oral Diseases. Shijiazhuang 050017, Hebei Province;
2. Hospital of Stomatology, NanKai University. Tianjin 300041, China

Received:2024-04-22 Revised:2024-07-12 Online:2025-08-25 Published:2025-08-26

摘要/Abstract

摘要： 目的：探讨牙源性间充质干细胞对成牙骨质细胞生物活性的影响及其在牙根吸收预防和治疗中的作用。方法：分离培养人牙周膜干细胞（periodontal ligament stem cells,PDLSCs）、牙髓干细胞（dental pulp stem cells,DPSCs）,利用流式细胞仪检测细胞表面标志物。将成牙骨质细胞OCCM-30与PDLSCs、DPSCs共培养12 h、24 h,采用CCK-8法检测其对成牙骨质细胞增殖活性的影响,碱性磷酸酶染色检测不同处理组成牙骨质细胞成骨能力的变化。采用细胞划痕试验和Transwell小室迁移试验比较成牙骨质细胞的迁移能力,利用酶联免疫吸附试验（ELISA）评估迁移相关蛋白基质金属蛋白酶9（matrix metalloprotease-9,MMP9）、基质金属蛋白酶13（matrix metalloprotease-13,MMP13）的表达变化。结果：PDLSCs的CD90、CD34表达率为99.44%、0.23%,DPSCs的CD90、CD34表达率为99.93%、3.47%,具有分化为成脂、成骨谱系的多能性。PDLSCs、DPSCs与成牙骨质细胞共培养,能促进成牙骨质细胞的增殖（P<0.05）。干细胞共培养处理使成牙骨质细胞的成骨能力增强。干细胞处理组成牙骨质细胞迁移能力显著增强（P<0.05）,MMP9、MMP13表达显著上调。结论：牙源性干细胞能促进成牙骨质细胞的增殖、矿化和迁移,在牙骨质再生修复中发挥积极作用,为牙根吸收的预防及治疗提供了新的思路。

关键词: 牙源性干细胞, 成牙骨质细胞, 增殖, 矿化, 迁移

Abstract: PURPOSE: To investigate the effect of dental-derived mesenchymal stem cells on the biological characteristics of cementoblasts. METHODS: Periodontal ligament stem cells (PDLSCs) and dental pulp stem cell (DPSCs) were isolated, flow cytometry was used to detect cell surface markers. Cementoblasts OCCM-30 were coculture with PDLSCs and DPSCs for 12 h and 24 h. Cell proliferation was examined using the CCK-8 method. ALP staining was used to evaluate the osteogenic differentiation of cementoblasts in different treatment groups. Wound healing migration assays and Transwell migration assays were performed to compare the migration of cementoblasts. The expression of MMP9 and MMP13 was assessed using enzyme-linked immunosorbent assay(ELISA). RESULTS: The expression rates of CD90 and CD34 of PDLSCs were 99.44% and 0.23%. The expression rates of CD90 and CD34 of DPSCs were 99.93% and 3.47%. Their multipotency were illustrated based on differentiation to abiogenic, osteogenic lineages. Human mesenchymal stem cells promoted the proliferation of cementoblasts(P<0.05). The osteogenic differentiation of cementoblasts was increased after stem cells treatment. The migration of cementoblasts was increased after stem cells treatment (P<0.05), and the expression of MMP9 and MMP13 were enhanced. CONCLUSIONS: Dental-derived stem cells can promote the proliferation, osteogenic differentiation and migration of cementoblasts, playing an active role in cementum restoration and providing a scientific basis for promoting the repair process in root resorption.

Key words: Dental-derived stem cells, Cementoblast, Proliferation, Osteogenic differentiation, Migration

中图分类号:

R783.5

李雯雯, 马文盛, 李颖辉, 赵圆, 李东娜, 翟浩嫣, 刘春艳. 牙源性间充质干细胞对成牙骨质细胞生物学特性的影响[J]. 上海口腔医学, 2025, 34(4): 354-361.

Li Wenwen, Ma Wensheng, Li Yinghui, Zhao Yuan, Li Dongna, Zhai Haoyan, Liu Chunyan. Effect of dental-derived stem cells on the biological characteristics of cementoblasts[J]. Shanghai Journal of Stomatology, 2025, 34(4): 354-361.

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牙源性间充质干细胞对成牙骨质细胞生物学特性的影响

Effect of dental-derived stem cells on the biological characteristics of cementoblasts

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