The effect of chronic kidney disease on periodontitis-related microorganisms in mice

doi:10.19439/j.sjos.2021.06.002

Abstract

Abstract: PURPOSE: To explore the effect of chronic kidney disease on the composition of oral microbial community in mice and find the significant species. METHODS: Twenty C57BL/6 male mice were randomly divided into 4 groups: healthy control group (HC), periodontitis group (PD), chronic kidney disease group (CKD) and chronic kidney disease and periodontitis group (CKD+PD). The periodontitis model was created in the fourth week when the chronic kidney disease model was established, and then the mice were sacrificed in the sixth week. Histopathological analysis of the kidney was performed by H-E staining and Masson's trichrome staining. Alveolar bone resorption of maxilla was analyzed by micro-CT analysis. The third-generation full-length sequencing of 16SrRNA gene was used to analyze the composition of oral microbial community among groups. Statistical analysis was performed using SPSS 24.0 software package. RESULTS: There were significant differences in alveolar bone resorption, the richness of species and the overall composition of the microbial community among the four groups (P<0.001). In CKD group, Streptococcus azizii had the most significant abundance. Escherichia coli was the most significantly different species among identifiable bacteria in CKD+PD group, while Staphylococcus lentus and Lactobacillus murinus were species with the most significant differences in HC group and PD group, respectively. CONCLUSIONS: The composition of the oral microbial community was significantly different among four groups with significant species.

Key words: Periodontitis, Chronic kidney disease, Species, The third-generation sequencing technology

CLC Number:

R781.4

MA Xiao-xin, DUAN Sheng-zhong, FENG Xi-ping, ZHANG Zhi-yuan, XU Wei, LU Hai-xia. The effect of chronic kidney disease on periodontitis-related microorganisms in mice[J]. Shanghai Journal of Stomatology, 2021, 30(6): 567-572.

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