Differential expression of pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia

doi:10.19439/j.sjos.2021.06.001

Abstract

Abstract: PURPOSE: To explore pro-angiogenic miRNAs differentially expressed in dental tissue-derived stem cells under hypoxia. METHODS: Stem cells from apical papilla, dental pulp stem cells and periodontal ligament stem cells were obtained from 3 patients, and treated with nomoxia or hypoxia for 1, 3, 5 d, respectively. Real-time PCR was performed to evaluate the expression of 9 specifically identified pro-angiogenic miRNAs and HIF-1α. SPSS 19.0 software package was used for statistical analysis. RESULTS: At the fifth day of hypoxic condition, as for SCAPs, the expression of miR-126 was relatively increased while miR-20a, miR-20b, miR-21, miR-130a, miR-132, miR-210 and miR-503 deceased. As for DPSCs, the expression of miR-21, miR-130a, miR-126 and miR-210 were relatively increased while miR-132 decreased. As for PDLSCs, the expression of miR-126, miR-21 and miR-296 were relatively increased. Finally, the expression of HIF-1α was relatively upregulated in all three type of cells. CONCLUSIONS: Pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia exists specific expression profile.

Key words: Dental tissue-derived stem cells, microRNA, Hypoxia, Expression profile

CLC Number:

R781.3

LIN Xin-hai, WANG Hao-dong, JIANG Long. Differential expression of pro-angiogenic miRNAs in dental tissue-derived stem cells under hypoxia[J]. Shanghai Journal of Stomatology, 2021, 30(6): 561-566.

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