睾酮水平对牙周炎小鼠模型炎症性骨吸收的影响及机制探讨

doi:10.19439/j.sjos.2020.04.009

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (4): 380-385.doi: 10.19439/j.sjos.2020.04.009

睾酮水平对牙周炎小鼠模型炎症性骨吸收的影响及机制探讨

武月霞¹, 司姗姗², 张昕³, 连克乾⁴

1.南方医科大学口腔医院正畸科,广东广州 510280;
2.南方医科大学口腔医院牙体牙髓病科,广东广州 510280;
3.南方医科大学口腔医院种植修复科,广东广州 510280;
4.中山大学附属第一医院口腔科,广东广州 510080

收稿日期:2019-11-25 修回日期:2020-01-02 出版日期:2020-08-25 发布日期:2020-09-11
通讯作者: 连克乾,E-mail：13682257988@163.com
作者简介:武月霞（1979-）,女,硕士,主治医师,E-mail：qq597748182@163.com

Effect and mechanism of testosterone level on inflammatory bone resorption in periodontitis with mice

WU Yue-xia¹, SI Shan-shan², ZHANG Xin³, LIAN Ke-qian⁴

1. Department of Orthodontics, Southern Medical University. Guangzhou 510280;
2. Department of Endodontics, Southern Medical University. Guangzhou 510280;
3. Department of Implantology and Prosthodontics, Stomatological Hospital, Southern Medical University. Guangzhou 510280;
4. Department of Stomatology, The First Affiliated Hospital of Sun Yat-sen University. Guangzhou 510080, Guangdong Province, China

Received:2019-11-25 Revised:2020-01-02 Online:2020-08-25 Published:2020-09-11

摘要/Abstract

摘要： 目的：探讨睾酮水平对牙周炎小鼠模型炎症性骨吸收的影响及机制。方法：48只SD小鼠随机分为未结扎组、假手术组、去势组、去势+睾酮组4组,每组各12只,分别进行空白处理、去势模型和牙周炎模型构建。于结扎术后6周采集小鼠内眦静脉血,测定血清睾酮水平。处死小鼠后,取左侧上颌骨组织,进行苏木精-伊红染色和亚甲蓝染色,比较各组小鼠牙槽骨丧失量（alveolar bone loss,ABL）和牙槽骨吸收面积。利用实时荧光定量PCR测定牙龈组织中炎性细胞因子mRNA的表达,同时对血清睾酮水平与ABL、牙槽骨吸收面积及细胞因子进行相关性分析。采用SPSS 20.0软件包对数据进行统计学分析。结果：假手术组、去势组和去势+睾酮组小鼠血清睾酮水平显著低于未结扎组（P<0.05）;去势+睾酮组小鼠血清睾酮水平显著高于假手术组和去势组（P<0.05）;去势组小鼠血清睾酮水平显著低于假手术组（P<0.05）;去势+睾酮组小鼠ABL显著大于未结扎组、假手术组和去势组（P<0.05）,去势组小鼠ABL显著小于假手术组（P<0.05）;去势+睾酮组小鼠牙槽骨吸收面积显著大于未结扎组、假手术组和去势组（P<0.05）,去势组小鼠牙槽骨吸收面积显著小于假手术组（P<0.05）;假手术组、去势组和去势+睾酮组小鼠牙龈组织中白介素1β（IL-1β）mRNA、白介素6（IL-6）mRNA及肿瘤坏死因子α（TNF-α）mRNA水平显著高于未结扎组,白介素10（IL-10）mRNA水平显著低于未结扎组（P<0.05）;假手术组和去势组小鼠牙龈组织中IL-1β mRNA水平显著低于去势+睾酮组,去势组显著低于假手术组（P<0.05）;血清睾酮水平与ABL、牙槽骨吸收面积、IL-1β呈正相关（P<0.05）。结论：牙周炎小鼠睾酮水平降低,睾酮长期耗竭状态可减少牙槽骨炎症性骨吸收,可能通过降低IL-1β水平而实现。合理降低睾酮水平,有可能成为减少牙周炎患者牙槽骨吸收的治疗新思路。

关键词: 牙周炎, 睾酮, 炎症, 骨吸收

Abstract: PURPOSE: To investigate the effect of testosterone level on inflammatory bone resorption in periodontitis with mice. METHODS: Forty-eight SD mice were randomly divided into unligated group, sham operation group, castration group, castration + testosterone group, 12 mice in each group. At 6 weeks after ligation, serum testosterone levels were measured, and alveolar bone loss (ABL) and alveolar bone absorption area were compared by hematoxylin - eosin staining and methylene blue staining. The expression of inflammatory cytokine messenger RNA(mRNA) in gingival tissue was measured by real-time fluorescence quantitative PCR. SPSS 20.0 software package was used to analyze the data. RESULTS: Serum testosterone level among four groups was the highest in the unligated group, followed by castration + testosterone group, sham operation group and castration group, with significant difference(P<0.05). The ABL of the castration + testosterone group was significantly larger than that of the unligated group, the sham operation group and the castration group(P<0.05). The ABL of the castration group was significantly smaller than that of the sham operation group (P<0.05). The alveolar bone resorption area of the castration + testosterone group was significantly larger than that of the unligated group, the sham operation group and the castration group(P<0.05). The alveolar bone resorption area of the castration group was significantly smaller than that of the sham operation group (P<0.05). Interleukin-1β (IL-1β) mRNA, interleukin-6(IL-6) mRNA and tumor necrosis factor-α(TNF-α) mRNA levels in gingival tissues of sham operation group, castration group and castration + testosterone group were significantly higher than the unligated group. The levels of interleukin-10(IL-10) mRNA in gingival tissues of sham operation group, castration group and castration + testosterone group were significantly lower than those in unligated group(P<0.05). The level of IL-1β mRNA in gingival tissues among four groups was the the highest in the unligated group, followed by castration + testosterone group, sham operation group and castration group, with significant difference (P<0.05). Serum testosterone levels were positively correlated with ABL, alveolar bone resorption area, and IL-1β (P<0.05). CONCLUSIONS: Periodontitis with mice have decreased testosterone levels, and long-term testosterone depletion can reduce inflammatory bone resorption in alveolar bone, which may be achieved by reducing the level of IL-1β, indicating that reduction of the level of testosterone in periodontitis patients may be a new treatment target for alveolar bone resorption.

Key words: Periodontitis, Testosterone, Inflammation, Bone resorption

中图分类号:

R781.4

武月霞, 司姗姗, 张昕, 连克乾. 睾酮水平对牙周炎小鼠模型炎症性骨吸收的影响及机制探讨[J]. 上海口腔医学, 2020, 29(4): 380-385.

WU Yue-xia, SI Shan-shan, ZHANG Xin, LIAN Ke-qian. Effect and mechanism of testosterone level on inflammatory bone resorption in periodontitis with mice[J]. Shanghai Journal of Stomatology, 2020, 29(4): 380-385.

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睾酮水平对牙周炎小鼠模型炎症性骨吸收的影响及机制探讨

Effect and mechanism of testosterone level on inflammatory bone resorption in periodontitis with mice

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