DEC1蛋白在口腔鳞癌中的表达及临床意义

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 217-220.

DEC1蛋白在口腔鳞癌中的表达及临床意义

游镜民，苏彤，夏昆，林璐，刘琼，朱腾飞

湖南省常德市第一人民医院口腔科,湖南常德 415003

收稿日期:2015-08-27 修回日期:2015-11-12 出版日期:2016-03-25 发布日期:2016-05-06
作者简介:游镜民(1985-),男,硕士,住院医师,E-mail:youjingmin85@163.com 苏彤, E-mail:sutongs@163.com

Expression of differentiated embryo-chondrocyte expressed gene l in oral squamous cell carcinoma and its clinical significance

YOU Jing-min, SU Tong, XIA Kun, LIN Lu, LIU qiong, ZHU Teng-fei

Department of Stomatology, First People's Hospital of Changde. Changde 415003, Hunan Province, China

Received:2015-08-27 Revised:2015-11-12 Online:2016-03-25 Published:2016-05-06

摘要/Abstract

摘要： 目的:研究软骨分化表达基因1(differentiated embryo-chondrocyte expressed gene l,DEC1)在口腔鳞癌中的表达,以期为口腔鳞癌的诊断和预后评估提供依据。方法:采用免疫组织化学方法检测80例口腔鳞癌组织及30例正常口腔黏膜组织中DEC1蛋白的表达,并分析相关的临床病理因素。采用SPSS10.0软件包对数据进行统计学分析。结果:口腔鳞癌组织中DEC1的阳性表达率为92.50％,正常口腔黏膜组织中DEC1的阳性表达率为6.67％,两者差异显著(P＜0.05)。DEC1的表达在高、中、低不同分化程度口腔鳞癌组织之间以及在有、无淋巴结转移的口腔鳞癌组织之间的差异具有统计学意义(P＜0.05)。结论:DEC1的表达上调可能与口腔鳞癌的发生、发展有关。

关键词: DEC1, 口腔鳞癌, 免疫组织化学

Abstract: PURPOSE: To investigate the expression of differentiated embryo-chondrocyte expressed gene l(DEC1)in oral squamous cell carcinoma(OSCC)and its clinical significance. METHODS: Detection of the protein expression of DEC1 in tissue specimens from 80 primary OSCC patients and 30 cases of normal oral mucosa were performed using immunohistochemistry. SPSS10.0 software package was uses for statistical analysis. RESULTS: The result of immunohistochemistry showed that positive expression rate of DEC1 in OSCC tissues was 92.50%, 5.00% in normal oral mucosa tissue. The difference was statistically significant (P<0.05). In OSCC tissues, the difference in expression of DEC1 was statistically significant (P<0.05) between highly differentiated group and lowly differentiated group, between patients with lymph node metastasis and patients without lymph node metastasis. CONCLUSIONS: The significantly increased DEC1 gene may be associated with the tumorigenesis and development of OSCC.

Key words: Differentiated embryo-chondrocyte expressed gene l, Oral squamous cell carcinoma, Immunohistochemistry

中图分类号:

R739.8

游镜民，苏彤，夏昆，林璐，刘琼，朱腾飞. DEC1蛋白在口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2016, 25(2): 217-220.

YOU Jing-min, SU Tong, XIA Kun, LIN Lu, LIU qiong, ZHU Teng-fei. Expression of differentiated embryo-chondrocyte expressed gene l in oral squamous cell carcinoma and its clinical significance[J]. Shanghai Journal of Stomatology, 2016, 25(2): 217-220.

参考文献

Shen M, Kawamoto T, Yan W, et al. Molecular characterization of the novel basic helix-loop-helix protein DEC1 expressed in differentiated human embryo chondrocytes. Biochem Biophys Res Commun, 1997, 236(2): 294-298.
Wu Y, Sato F, Yamada T, et al. The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer. Int J Oncol, 2012, 41(4): 1337-1346.
Liu Y, Miao Y, Wang J, et al. DEC1 is positively associated with the malignant phenotype of invasive breast cancers and negatively correlated with the expression of claudin-1. Int J Mol Med, 2013, 31(4): 855-860.
Noshiro M, Usui E, Kawamoto T, et al. Liver X receptors (LXRalpha and LXRbeta) are potent regulators for hepatic Dec1 expression . Genes Cells, 2009, 14(1): 29-40. Jemal A, Bray F, Center MM, et al. Global cancer statistics . CA Cancer J Clin, 2011, 61(2): 69-90.
Wiltshire S, Frayling TM, Hattersley AT, et al. Evidence for linkage of stature to chromosome 3p26 in a large U.K. Family data set ascertained for type 2 diabetes. Am J Hum Genet, 2002, 70(2): 543-546.
Teramoto M, Nakamasu K, Noshiro M, et al. Gene structure and chromosomal location of a human bHLH transcriptional factor DEC1 x Stra13 x SHARP-2/BHLHB2 . J Biochem, 2001, 129(3): 391-396.
Yamada K, Miyamoto K. Basic helix-loop-helix transcription factors, BHLHB2 and BHLHB3; their gene expressions are regulated by multiple extracellular stimuli . Front Biosci, 2005, 10: 3151-3171.
Davis RL, Turner DL. Vertebrate hairy and enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning . Oncogene, 2001, 20(58): 8342-8357.
Li Y, Zhang H, Xie M, et al. Abundant expression of Dec1/stra13/sharp2 in colon carcinoma: its antagonizing role in serum deprivation-induced apoptosis and selective inhibition of procaspase activation . Biochem J, 2002, 367(Pt 2): 413-422.
Giatromanolaki A, Koukourakis MI, Sivridis E, et al. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer . J Pathol, 2003, 200(2): 222-228.
Zheng Y, Shi X, Wang M, et al. The increased expression of DEC1 gene is related to HIF-1alpha protein in gastric cancer cell lines . Mol Biol Rep, 2012, 39(4): 4229-4236.
Yoon DY, Buchler P, Saarikoski ST, et al. Identification of genes differentially induced by hypoxia in pancreatic cancer cells . Biochem Biophys Res Commun, 2001, 288(4): 882-886.
Bhawal UK, Ito Y, Tanimoto K, et al. IL-1beta-mediated up-regulation of DEC1 in human gingiva cells via the Akt pathway . J Cell Biochem, 2012, 113(10): 3246-3253.
Shi XH, Zheng Y, Sun Q, et al. DEC1 nuclear expression: a marker of differentiation grade in hepatocellular carcinoma . World J Gastroenterol, 2011, 17(15): 2037-2043.
Liu Y, Wang L, Lin XY, et al. The transcription factor DEC1 (BHLHE40/STRA13/SHARP-2) is negatively associated with TNM stage in non-small-cell lung cancer and inhibits the proliferation through cyclin D1 in A549 and BE1 cells. Tumour Biol, 2013, 34(3): 1641-1650.

[1]	谢玉婷, 熊屏. 基于单原子铁纳米催化剂的纳米催化疗法对口腔鳞癌细胞Cal27的作用[J]. 上海口腔医学, 2024, 33(2): 117-122.
[2]	段咏华, 吴开柳, 梅健, 周谊渊, 刘梦石. 口腔鳞癌组织中RACK1与EGP40的表达及其与临床病理特征及预后的相关性[J]. 上海口腔医学, 2023, 32(4): 385-390.
[3]	董淋升, 唐进, 程维, 冉婧璇, 刘兆博, 李雅冬. IL-6和β-catenin在口腔鳞癌中的表达及预后价值[J]. 上海口腔医学, 2023, 32(3): 280-286.
[4]	胡小媛, 王宁, 张晓野, 杨茜, 高菲, 王成勤, 尚伟, 项锋钢, 冯元勇. Chemerin对口腔鳞癌中性粒细胞浸润的影响及机制探讨[J]. 上海口腔医学, 2023, 32(2): 158-165.
[5]	郭梦瑶, 李凯玉, 聂敏海, 刘旭倩. 上调Decorin对口腔鳞癌荷瘤裸鼠EGFR、C-Myc、p21表达的影响[J]. 上海口腔医学, 2023, 32(1): 40-46.
[6]	文旭涛, 马振, 张翀, 陶识丞, 陈兴金, 麦华明. 不同培养条件对CD133⁺原代口腔鳞癌细胞干性维持的影响[J]. 上海口腔医学, 2022, 31(4): 343-348.
[7]	秦志明, 揭伟萍, 池彦廷, 李宏锋, 李斌斌. 基于局部可变阈值的无监督定量Ki-67细胞增殖指数模型的初步建立[J]. 上海口腔医学, 2022, 31(3): 248-254.
[8]	买尔哈巴·买合木提, 拜婕, 如斯坦木·依米提, 姚志涛. 50例口腔鳞癌患者治疗过程中营养状况改变及影响因素分析[J]. 上海口腔医学, 2022, 31(2): 205-210.
[9]	谈亦然, 赵铜超, 琚梧桐, 钟来平. 长春新碱对口腔鳞癌中Stathmin调控作用的实验研究[J]. 上海口腔医学, 2022, 31(1): 1-5.
[10]	谢非, 秦星, 童桐, 蒋英英, 张建军. IGF2BP1在口腔鳞癌细胞耐药中的作用及机制探讨[J]. 上海口腔医学, 2021, 30(5): 456-461.
[11]	李蕾, 李晓东, 庄乾伟, 葛良玉, 李志萍, 顾倩平, 白玉婷, 孟箭. 超声热疗联合TPF化疗治疗19例老年晚期口腔鳞癌的疗效分析[J]. 上海口腔医学, 2021, 30(5): 543-547.
[12]	邵小钧, 朱乔, 刘有, 韩小东, 林小臻, 席庆. 咀嚼槟榔与口腔鳞癌的相关临床病理学因素分析[J]. 上海口腔医学, 2021, 30(3): 268-272.
[13]	薛佳艺, 朱晓燕, 危常磊, 孙培, 张慧, 邓婧. 25例口腔鳞癌患者唾液代谢组学分析[J]. 上海口腔医学, 2021, 30(2): 201-205.
[14]	王树斌, 秦帅华, 易雅群, 李新明. 216例口腔鳞癌患者术后心理困扰影响因素及放疗前、后生存质量评价[J]. 上海口腔医学, 2021, 30(1): 71-76.
[15]	傅宗云, 陶峰. MMP-14、ING4和HIF-1α在60例口腔鳞癌中的表达及临床意义[J]. 上海口腔医学, 2020, 29(6): 642-646.