上海口腔医学

上海口腔医学 ›› 2016, Vol. 25 ›› Issue (2): 212-216.

• 论著 • 上一篇    下一篇

GDNF对唾液腺腺样囊性癌嗜神经侵袭中基质降解和黏附能力的影响

郑善川1*，张玉茹2，骆树瑜2   

  1. 1.天津市武清区人民医院 口腔科,天津 301700；
    2.天津医科大学口腔医院 病理科,天津 300070
  • 收稿日期:2015-06-15 修回日期:2015-09-26 出版日期:2016-03-25 发布日期:2016-05-06
  • 作者简介:郑善川(1983-),男,硕士,E-mail:zhshchuan2008@126.com 骆树瑜,Tel:022-23332068,E-mail:luoshuyubaobei@163.com
  • 基金资助:
    天津医科大学口腔医院科研课题(2013YKYQ01)

The effect of GDNF on matrix-degrading and cell-adhesion during perineural invasion of salivary adenoid cystic carcinoma

ZHENG Shan-chuan1, ZHANG Yu-ru2, LUO Shu-yu2   

  1. 1. Department of Stomatology, Wuqingqu People's Hospital. Tianjin 301700;
    2. Department of Oral Pathology, Stomatology Hospital, Tianjin Medical University. Tianjin 300070,China
  • Received:2015-06-15 Revised:2015-09-26 Online:2016-03-25 Published:2016-05-06

RichHTML

0

PDF (PC)

275

被引次数

1

摘要： 目的:探讨GDNF对唾液腺腺样囊性癌(adenoma cystic carcinoma,ACC)嗜神经侵袭(perineural invasion,PNI)中基质降解和黏附能力变化的影响。方法:应用免疫组织化学染色SP法对42例ACC和5例正常唾液腺组织中GDNF、MMP-9、NF-κB、整合素β1的表达进行检测。采用SPSS16.0软件包对数据进行统计学分析。结果:GDNF在ACC肿瘤细胞的细胞质和肿瘤周围的神经纤维中呈阳性表达。 NF-κB、MMP-9、整合素β1在ACC肿瘤细胞的细胞质均呈阳性表达,且可见NF-κB表达于细胞核,整合素β1表达于细胞膜,阳性表达率分别为69.05%(29/42)、66.67%(28/42)和61.90%(26/42)。NF-κB、MMP-9、整合素β1的表达在PNI组和非PNI组间差异显著,P值分别为0.005、0.011、0.001。GDNF与NF-κB、MMP-9、整合素β1表达呈正相关,r=0.443,P=0.003;r=0.401,P=0.009;r=0.535,P=0.000。MMP-9、整合素β1与NF-κB表达呈正相关,r=0.501,P=0.001;r=0.429, P=0.005。MMP-9的表达与整合素β1表达呈正相关,r=0.381, P=0.013。结论:GDNF在 ACC嗜神经侵袭中促进基质降解和黏附能力改变。NF-κB、MMP-9、整合素β1的表达与GDNF关系密切,共同促使ACC神经侵袭的发生与进展。

关键词: 腺样囊性癌, 基质金属蛋白酶9, 核因子κB, 胶质细胞源性神经生长因子, 嗜神经侵袭, 整合素β1

Abstract: PURPOSE: To investigate the possible contribution of GDNF in matrix-degrading, cell-adhesion during perineural invasion (PNI) of salivary adenoid cystic carcinoma (ACC). METHODS: Totally 42 ACCs and 5 normal salivary tissues were included in the present study.Immunohistochemical staining SP method was used to detect the expression of GDNF,MMP-9,NF-κB,integrin β1 in ACC specimens and normal salivary tissues. Statistical analysis was performed using SPSS16.0 software package. RESULTS:GDNF was strongly expressed in ACC tumor cells and nerve fibers adjacent to ACC tumor cells. NF-κB, MMP-9, integrin β1 were positively expressed in ACC cell cytoplasm, integrin β1 was also found in ACC cell membrane, and NF-κB in nuclei occasionally. The positive expression rate was 69.05%(29/42),66.67%(28/42),61.90%(26/42), respectively. The differences between the expression of NF-κB, MMP-9, integrinβ1 in PNI group and non-PNI group were significant (P=0.005,P=0.011,P=0.001, respectively). Expression of NF-κB, MMP-9, integrin β1 was correlated to that of GDNF(r=0.443, P=0.003; r=0.401, P=0.009; r=0.535, P=0.000, respectively). Expression of MMP-9 and integrin β1 was positively correlated to that of NF-κB(r=0.501, P=0.001; r=0.429, P=0.005). Expression of MMP-9 was correlated positively to that of integrin β1 (r=0.381, P=0.013). CONCLUSIONS:GDNF may increase the matrix-degrading and cell-adhesion of ACC in the process of PNI. NF-κB, MMP-9 and integrin β1 involve in ACC cells invading nerves.

Key words: Adenoid cystic carcinoma, MMP-9, NF-κB, Glial cell line-derived neurotrphic growth factor, Perineural invasion, Integrin β1

中图分类号: