不同龋敏感儿童牙菌斑中4种致龋菌的动态监测分析

上海口腔医学 ›› 2015, Vol. 24 ›› Issue (3): 321-325.

不同龋敏感儿童牙菌斑中4种致龋菌的动态监测分析

褚昊月,孙静,张颖,郭静,亓庆国

山东大学口腔医学院牙体牙髓病科;山东省口腔生物医学重点实验室,山东济南 250012

收稿日期:2015-01-12 出版日期:2015-06-20 发布日期:2015-07-24
通讯作者: 亓庆国,Tel:0531-88382213,E-mail:qqg@sdu.edu.cn
作者简介:褚昊月（1989-）,女,硕士研究生,E-mail:chuhaoyue123@163.com
基金资助:
国家自然科学基金（81371158）; 卫生公益性行业科研专项基金（201002017）

Dynamic and quantitative analysis of cariogenic bacteria and its proportion in the dental plaque of different caries-susceptible children

CHU Hao-yue,SUN Jing,ZHANG Ying,GUO Jing,QI Qing-guo

Department of Endodontics, School of Stomatology, Shandong University;Key Lab of Oral Biomedicine of Shandong Province. Jinan 250012, Shandong Province, China

Received:2015-01-12 Online:2015-06-20 Published:2015-07-24
Supported by:
National Natural Science Foundation of China (81371158) and Special Research Fund for Health Care Industry (201002017)

摘要/Abstract

摘要： 目的:监测无龋儿童组（caries free,CF）、低龄儿童龋组（early childhood caries,ECC）和重度低龄儿童龋组 (severe early childhood caries,S-ECC)牙菌斑中4种致龋菌的动态变化。方法:选取60名3~5岁儿童,对其进行龋病检查和牙菌斑样本收集,追踪观察1 a。采用实时荧光定量PCR技术对2次取样的4种致龋菌（变形链球菌、远缘链球菌、嗜酸乳杆菌和内氏放线菌）进行定量检测,计算各致龋菌所占总菌的比例。采用SPSS17.0软件包对实验数据进行χ²检验和配对t检验。结果:在基线及1 a后患龋率分别为66.7%和81.7%,差异无显著性（χ²=1.76,P>0.01）;龋失补牙面数分别为（5.80±2.53）和（7.90±1.76）,差异显著（t=3.51,P<0.01）。ECC、S-ECC组中,4种致龋菌所占比例在基线和1 a后有显著差异（P<0.01）。1 a后ECC、S-ECC组中变形链球菌、远缘链球菌之和所占比例显著高于基线水平（P<0.01）。变异链球菌与远缘链球菌之和所占总菌的比例与dmfs指数的变化在基线和1 a后均具有良好的线性关系。结论:菌斑中致龋菌的比例变化与低龄儿童龋密切相关,致龋菌所占总菌的比例越高,低龄儿童龋易感性就越高。

关键词: 低龄儿童龋, 牙菌斑, 致龋菌, 实时荧光定量PCR

Abstract: PURPOSE: To investigate the ratio and change of bacteria in dental plaque in different caries-susceptible children. METHODS: Dental plaques were obtained from 60 children of 3-5 years old. Four targeted cariogenic bacteria were quantitatively detected by real-time PCR and the mean dmft score was calculated. The effectiveness was assessed and compared using SASS.17 software package. RESULTS: The decayed missing filled surfaces(dmfs) score and the percentages of cariogenic bacteria at 1 year increased significantly(P<0.01). There were significant differences in percentages of S.mutans and S.sobrinus in early childhood caries group and severe early childhood caries group between baseline and at 1 year (P<0.01). There was linear correlation between dmfs score and the sum of the percentages of S.mutans and S.sobrinus at baseline and 1 year. CONCLUSIONS: The ratios of cariogenic bacteria to total bacteria in plaque are closely associated with the prevalence of dental caries in children. The higher proportion of cariogenic bacteria, the higher susceptibility of subjects with early childhood caries.

Key words: Early childhood caries, Plaque, Cariogenic bacteria, Real-time PCR

中图分类号:

R788.1

褚昊月,孙静,张颖,郭静,亓庆国. 不同龋敏感儿童牙菌斑中4种致龋菌的动态监测分析[J]. 上海口腔医学, 2015, 24(3): 321-325.

CHU Hao-yue,SUN Jing,ZHANG Ying,GUO Jing,QI Qing-guo. Dynamic and quantitative analysis of cariogenic bacteria and its proportion in the dental plaque of different caries-susceptible children[J]. Shanghai Journal of Stomatology, 2015, 24(3): 321-325.

参考文献

[1] Seow WK, Clifford H, Battistutta D, et al. A case-control study of early childhood caries in Australia [J]. Caries Res, 2009, 43(1): 25-35.
[2] Corhv PM, Lyons- Weiler J, Bretz WA, et al. Microbial risk indicators of early childhood caries[J]. J Clin microbial,2005,43(11): 5753-5759.
[3] 李明博, 仪虹, 尹丽雪, 等. 唾液中放线菌与儿童龋病相关性的定量检测研究 [J]. 现代口腔医学杂志, 2012, 26(2): 76-79.
[4] Li Y, Ge Y, Saxena D, et al. Genetic profiling of the oral microbiota associated with severe early-childhood caries[J]. J Clin Microbiol, 2007,45(1): 81-87.
[5] Choi EJ, Lee SH, Kim YJ. Quantitative real-time polymerase chain raction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries [J]. Int J Paediatr Dent, 2009, 19(2): 141-147.
[6] Sinsimer D, Leekha S, Park S, et al. Use of a multiplex molecular beacon platform for rapid detection of methicillin and vancomycin resistance in Staphylococcus aureus [J]. J Clin Microbiol, 2005, 43(9): 4585-4591.
[7] 中国疾病预防控制中心. 第三次全国口腔健康流行病学抽样调查结果[R/OL]. http://www.chinacdc.cn[2007-06-15].
[8] Okada M, Kawamura M, Oda Y, et al. Caries prevalence associated with Streptococcus mutans and Streptococus sobrinus in Japanese schoolchildren[J]. Int J Paediate Dent, 2012, 22(5): 342-348.
[9] Seki M, Yamashita Y, Shibata Y, et al. Effect of mixed mutans Streptococci colonization on caries development [J]. Oral Microbiol Immunol, 2006, 21(1): 47-52.
[10] 梁景平, 姜云涛, 李超伦, 等. 实时PCR技术在龋易感儿童牙菌斑致龋菌定量检测中的应用[J]. 上海交通大学学报(医学版), 2007, 27(2): 128-132.
[11] Takahashi N, Nyvad B. The role of bacteria in the caries process: ecological perspectives [J]. J Dent Res, 2011, 90(3): 294-303.
[12] Childers NK, Osgoog RC, Hsu K-L, et al. Real-time quantitative polymerase chain reaction for enumeration of Streptococcus mutans from oral samples [J]. Eur J Oral Sci , 2011, 119(6): 447-454.