不同去势时间大鼠牙槽骨微结构变化的Micro-CT研究

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (6): 641-645.

• 基础研究 • 下一篇

不同去势时间大鼠牙槽骨微结构变化的Micro-CT研究

代庆刚, 房兵, 张鹏, 杨筱, 王洁, 欧阳宁鹃, 吴玉琼, 江凌勇

上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科,上海市口腔医学重点实验,上海 200011

收稿日期:2014-05-04 修回日期:2014-07-09 出版日期:2014-12-20 发布日期:2015-01-08
通讯作者: 江凌勇,Tel：021-23271699,E-mail：jly117@sina.com E-mail:daiqinggang@126.com
作者简介:代庆刚（1987-）,男,博士研究生
基金资助:
国家自然科学基金（81371121,11342005,30901698, 10972142）; 上海市科学技术委员会资助项目（13ZR1423700,12JC1405700）; 上海交通大学医工交叉基金（YG2012MS40 ); SMC-晨星青年学者奖励计划优秀青年教师（B类计划）; 上海市教育委员会创新团队项目

Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats

DAI Qing-gang, FANG Bing, ZHANG Peng, YANG Xiao, WANG Jie, OUYANG Ning-juan, WU Yu-qiong, JIANG Ling-yong

Department of Oral and Craniomaxillofacial Science, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-05-04 Revised:2014-07-09 Online:2014-12-20 Published:2015-01-08
Supported by:
National Natural Science Foundation of China (81371121, 11342005, 30901698, 10972142); Natural Science Foundation of Shanghai Municipality (13ZR1423700), Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality (12JC1405700); Collaborative Foundation of Medical and Engineering Science of Shanghai Jiao Tong University (YG2012MS40), “Chen Xing” Project from Shanghai Jiao Tong University and Innovative Research Team of Shanghai Municipal Education Commission

摘要/Abstract

摘要： 目的：观察不同去势时间大鼠牙槽骨微结构的改变,探讨去势时间对牙槽骨骨质疏松程度的影响。方法：健康未孕雌性SD大鼠24只,随机分为去势组(OVX)与假手术组（sham）,每组各12只,分别在全麻下行双侧卵巢去势术与假手术。于术后3个月及6个月2个不同时间点分别处死每组大鼠各6只,取右侧上颌骨标本行Micro-CT扫描;观察去势3个月及6个月后大鼠上颌第一磨牙根分叉区牙槽骨微结构的改变。采用SPSS16.0软件包对数据进行统计学分析。结果： Micro-CT水平面、矢状面二维图像及三维重建均显示,去势3个月及6个月大鼠牙槽骨骨髓腔面积增加、骨小梁变细、部分发生断裂。骨结构参数分析显示,去势3个月及6个月后,大鼠牙槽骨骨密度（BMD）及骨体积分数（BV/TV）、骨小梁宽度（Tb.Th）均显著低于相应时间假手术大鼠（P<0.05）;去势6个月组较3个月组大鼠牙槽骨骨密度(BMD)、骨体积分数(BV/TV)、骨小梁宽度(Tb.Th)显著降低（P<0.05）。去势3个月及6个月大鼠骨小梁数目（Tb.N）及骨小梁分离度（Tb.Sp）显著高于相应假手术组（P<0.05）,且去势6个月组骨小梁数目(Tb.N)显著高于去势3个月组（P<0.05）。结论：随着去势时间延长,大鼠牙槽骨骨量降低、骨小梁微结构破坏加剧,骨质疏松程度加重。

关键词: 去势, 牙槽骨, 骨质疏松, 微结构, Micro-CT

Abstract: PURPOSE: To study the changes of microarchitecture of alveolar bone due to different duration of ovariectomy in rats. METHODS: Twenty-four virgin Sprague-Dawley rats were randomly assigned to ovariectomy group (OVX) or sham-ovariectomy group (sham). OVX rats were subjected to bilateral ovariectomy and sham-ovariectomy was conducted in sham rats. Six rats of each group were sacrificed respectively 3 months and 6 months after surgery. The right semi-maxilla of all rats were scanned by Micro-CT, and the inter-radicular alveolar bone of the maxillary first molar was analyzed. Statistical analysis was carried out with SPSS 16.0 software package. RESULTS: Two-dimensional and three-dimensional reconstructed images of the alveolar bone showed porotic changes in rats both 3 months and 6 months after ovariectomy, including thinner, looser trabeculae and expanded bone marrow. When compared with corresponding sham rats, BMD, BV/TV and Tb.Th of alveolar bone significantly decreased in OVX rats both 3 months and 6 months after ovariectomy (P<0.05). Tb.N and Tb.Sp significantly increased in both OVX groups (P<0.05). When compared with 3 months after ovariectomy, the rats 6 months after ovaroectomy shared deceased BMD, BV/TV and Tb.Th (P<0.05) and increased Tb.N (P<0.05). CONCLUSIONS: Bone loss and deterioration of trabeculae of alveolar bone aggravates with the extended duration of ovariectomy in OVX rats.

Key words: Ovariectomy, Alveolar bone, Osteoporosis, Microarchitecture, Micro-CT

中图分类号:

R782.1

代庆刚, 房兵, 张鹏, 杨筱, 王洁, 欧阳宁鹃, 吴玉琼, 江凌勇. 不同去势时间大鼠牙槽骨微结构变化的Micro-CT研究[J]. 上海口腔医学, 2014, 23(6): 641-645.

DAI Qing-gang, FANG Bing, ZHANG Peng, YANG Xiao, WANG Jie, OUYANG Ning-juan, WU Yu-qiong, JIANG Ling-yong. Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats[J]. Shanghai Journal of Stomatology, 2014, 23(6): 641-645.

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不同去势时间大鼠牙槽骨微结构变化的Micro-CT研究

Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats

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