高糖环境通过抑制CXCR-4影响大鼠下颌骨骨髓基质细胞迁移

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (6): 646-650.

高糖环境通过抑制CXCR-4影响大鼠下颌骨骨髓基质细胞迁移

张博^*, 刘娜^*, 顾斌, 吴昊, 高羽萱, 王东胜, 刘洪臣

解放军总医院口腔医学研究所,北京 100853

收稿日期:2014-03-14 修回日期:2014-04-08 出版日期:2014-12-20 发布日期:2015-01-08
通讯作者: 刘洪臣,Tel：010-66936254,E-mail：liuhongchenpla301@163.com E-mail:hugo_zhang_005@163.com;liunapla301 @163.com
作者简介:张博（1981-）,男,在读博士研究生,医师；刘娜(1981-),女,博士,主治医师。*并列第一作者
基金资助:
国家自然科学基金（81271180, 31200741）

Effect of high glucose on migration of BMSCs through inhibiting CXCR-4

ZHANG Bo, LIU Na, GU Bin, WU Hao, GAO Yu-xuan, WANG Dong-sheng, LIU Hong-chen

Stomatological Center, General Hospital of PLA. Beijing 100853, China

Received:2014-03-14 Revised:2014-04-08 Online:2014-12-20 Published:2015-01-08

摘要/Abstract

摘要： 目的：探讨高糖环境通过抑制CXCR-4对大鼠下颌骨骨髓基质细胞迁移的影响。方法：从Wistar大鼠下颌骨中分离、培养骨髓基质细胞,利用Transwell实验筛选在生理糖浓度（5.5 mmol/L）下的SDF-1最适迁移浓度,检测不同糖浓度（5.5、16.5 mmol/L ）条件下SDF-1、AMD3100对细胞迁移能力的影响。利用Western 免疫印迹检测不同糖浓度（5.5、16.5 mmol/L ）条件下CXCR-4的表达,实时定量PCR检测CXCR-4、MMP-2的mRNA表达。采用SPSS 11.0软件包对数据进行统计学分析。结果： Transwell检测显示,生理糖浓度下,骨髓基质细胞的迁移能力对趋化因子SDF-1呈浓度依赖性;在生理糖浓度下,趋化因子SDF-1的最适浓度为100 ng/mL。高糖环境可抑制骨髓基质细胞的迁移能力。在不同糖浓度下,SDF-1均可明显增强细胞的迁移能力。加入AMD3100后,这种增强趋势受到明显抑制。高糖环境抑制CXCR-4蛋白以及CXCR-4和MMP-2 mRNA表达。结论：高糖环境可以通过抑制CXCR-4的表达来影响大鼠下颌骨骨髓基质细胞在 SDF-1/CXCR-4生物轴调控下的定向迁移能力。

关键词: 高糖, 骨髓基质细胞, 迁移, SDF-1/CXCR-4生物轴

Abstract: PURPOSE: To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4. METHODS: Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Transwell assay in physiological glucose concentration (5.5 mmol/L). In the optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package was used for statistical analysis. RESULTS: The optimum concentration of SDF-1 was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different concentrations of glucose, SDF-1 could promote the migration of BMSCs, but AMD3100 could inhibit this promotion. High glucose condition could inhibit the secretion of CXCR-4 and mRNA expression of CXCR-4 and MMP-2. CONCLUSIONS: High glucose inhibits migration of BMSCs by inhibiting CXCR-4 through SDF-1/CXCR-4 pathway.

Key words: Supported by National Natural Science Foundation of China (81271180, 31200741).

中图分类号:

R708.2

张博, 刘娜, 顾斌, 吴昊, 高羽萱, 王东胜, 刘洪臣. 高糖环境通过抑制CXCR-4影响大鼠下颌骨骨髓基质细胞迁移[J]. 上海口腔医学, 2014, 23(6): 646-650.

ZHANG Bo, LIU Na, GU Bin, WU Hao, GAO Yu-xuan, WANG Dong-sheng, LIU Hong-chen. Effect of high glucose on migration of BMSCs through inhibiting CXCR-4[J]. Shanghai Journal of Stomatology, 2014, 23(6): 646-650.

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