串珠素在大鼠牙髓损伤修复过程中的时空表达

上海口腔医学 ›› 2014, Vol. 23 ›› Issue (1): 15-19.

串珠素在大鼠牙髓损伤修复过程中的时空表达

李桥¹, 孙虹², 潘乙怀², 刘传通³

1.温州医科大学附属口腔医院儿童口腔科, 浙江温州 325027;
2.温州医科大学附属口腔医院牙体牙髓病科, 浙江温州 325027;
3.温州医科大学附属口腔医院口腔种植科, 浙江温州 325027

收稿日期:2013-04-12 修回日期:2013-05-20 出版日期:2014-02-20 发布日期:2014-10-21
通讯作者: 刘传通,E-mail: 773502228@qq.com
作者简介:李桥(1983-),男,硕士,住院医师,E-mail: f8_104@163.com
基金资助:
浙江省教育厅科研计划项目(20051196); 温州市科技计划项目(Y20100015)

Temporal and spacial expression of perlecan in dental pulp after injury

LI Qiao¹, SUN Hong², PAN Yi-huai², LIU Chuan-tong³

1.Department of Pediatric Dentistry, School and Hospital of Stomatology, Wenzhou Medical University. Wenzhou 325027, Zhejiang Province, China;
2.Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University. Wenzhou 325027, Zhejiang Province, China;
3.Department of Oral Implantology, School and Hospital of Stomatology, Wenzhou Medical University. Wenzhou 325027, Zhejiang Province, China

Received:2013-04-12 Revised:2013-05-20 Online:2014-02-20 Published:2014-10-21
Supported by:
Science and Technology Research Project of Department of Education of Zhejiang Province (20051196) and Wenzhou City (Y20100015)

摘要/Abstract

摘要： 目的：研究大鼠牙髓损伤修复过程中串珠素的时空表达变化及作用机制。方法：选用24只雄性SD大鼠,通过暴露一侧大鼠上颌磨牙牙髓建立牙髓损伤模型,对侧未损伤磨牙作为对照组,术后1、3、5、7 d取标本,脱钙后石蜡包埋,近远中向沿牙体长轴4 μm切片,采用免疫组织化学染色观察串珠素和β1整合素的表达变化,平均吸光度法计算相对表达量,采用SPSS19.0软件包对数据进行单因素方差分析。结果：与对照组相比,实验组串珠素染色强度增加,阳性染色位置随着炎症损伤的进展方向而转移,各组平均光密度均高于对照组(F=9.511,P<0.05),并以1 d组升高最为明显。β1整合素染色在牙髓损伤后3 d开始,在炎症牙髓和相对正常牙髓中阳性表达,3、5、7 d组的平均吸光度均显著高于对照组(F=124.18,P<0.05)。结论：串珠素在牙髓组织损伤后防御机制中可能通过调节血管生成和炎症细胞浸润而发挥作用。

关键词: 串珠素, β, 1整合素, 免疫组化, 大鼠, 牙髓损伤

Abstract: PURPOSE: To investigate the temporal and special expressions and functions of perlecan in dental pulp of rats after injury. METHODS: Specimens of one side of maxillary molars of 24 male SD rats were collected 1,3,5,7 days after injury, and the other side were used as controls. The specimens were decalcified and embedded in paraffin, sliced by longitudinal section and stained by immunohistochemistry. The staining of perlecan and β1 integrin were examined, and the average optical density (AOD) was compared. The measurement data were analyzed by ANOVA using SPSS 19.0 software package. RESULTS: The staining intensity of perlecan in dental pulp in the experimental group was significantly higher compared with that of the control group. The immunolocalization of perlecan in dental pulp was gradually progressed as the inflamed tissue was progressing after injury, and its AOD was significantly higher than that of control group (F=11.104,P<0.05), especially in the group of 1 d. The staining of β1 integrin was generally enhanced in the pulp 3 d after injury, and its AOD was significantly higher than that of control group (F=124.18,P<0.05). CONCLUSIONS: Perlecan may play an important role in tissue defense mechanism after injury through regulating angiogenesis and inflammatory cell infiltration.

Key words: Perlecan, β1 integrin, Immunohistochemistry, Rat, Dental pulp injury

中图分类号:

R781.3

李桥, 孙虹, 潘乙怀, 刘传通. 串珠素在大鼠牙髓损伤修复过程中的时空表达[J]. 上海口腔医学, 2014, 23(1): 15-19.

LI Qiao, SUN Hong, PAN Yi-huai, LIU Chuan-tong. Temporal and spacial expression of perlecan in dental pulp after injury[J]. Shanghai Journal of Stomatology, 2014, 23(1): 15-19.

参考文献

[1] Whitelock JM, Melrose J, Iozzo RV. Diverse cell signaling events modulated by perlecan [J]. Biochemistry, 2008, 47(43): 11174-11183.
[2] Zoeller JJ, McQuillan A, Whitelock J, et al. A central function for perlecan in skeletal muscle and cardiovascular development [J]. J Cell Biol, 2008, 181(2): 381-394.
[3] Pitol DL, Caetano FH, Lunardi LO. Microwave-induced fast decalcification of rat bone for electron microscopic analysis: an ultrastructural and cytochemical study[J].Braz Dent J,2007,18(2): 153-157.
[4] Iozzo RV, Sanderson RD. Proteoglycans in cancer biology, tumour microenvironment and angiogenesis[J]. J Cell Mol Med,2011,15(5): 1013-1031.
[5] Ida-Yonemochi H, Ohshiro K, Swelam W, et al. Perlecan, a basement membrane-type heparan sulfate proteoglycan, in the enamel organ: its intraepithelial localization in the stellate reticulum [J]. J Histochem Cytochem, 2005, 53(6): 763-772.
[6] Ida-Yonemochi H, Satokata I, Ohshima H, et al. Morphogenetic roles of perlecan in the tooth enamel organ: an analysis of overexpression using transgenic mice[J]. Matrix Biol,2011,30(7-8): 379-388.
[7] 陆群, 郭敬俊, 吴补领, 等. 胶质源性神经营养因子在正常和炎症牙髓组织中的变化 [J]. 牙体牙髓牙周病学杂志, 2000, 10(4): 201-203.
[8] García de Yébenes E, Ho A, Damani T, et al. Regulation of the heparan sulfate proteoglycan, perlecan, by injury and interleukin-1alpha [J]. J Neurochem, 1999, 73(2): 812-820.
[9] Leadbeater WE, Gonzalez AM, Logaras N, et al. Intracellular trafficking in neurones and glia of fibroblast growth factor-2, fibroblast growth factor receptor 1 and heparan sulphate proteoglycans in the injured adult rat cerebral cortex [J]. J Neurochem, 2006, 96(4): 1189-1200.
[10] Hauser PJ, Dozmorov MG, Bane BL, et al. Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis [J]. J Urol, 2008, 179(2): 764-769.
[11] Tran PK, Agardh HE, Tran-Lundmark K, et al. Reduced perlecan expression and accumulation in human carotid atherosclerotic lesions [J]. Atherosclerosis,2007,190(2): 264-270.
[12] Tran-Lundmark K, Tran PK, Paulsson-Berne G, et al. Heparan sulfate in perlecan promotes mouse atherosclerosis: roles in lipid permeability, lipid retention, and smooth muscle cell proliferation[J]. Circ Res, 2008, 103(1): 43-52.
[13] Aviezer D, Hecht D, Safran M, et al. Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis [J]. Cell, 1994, 79(6): 1005-1013.
[14] Ichimaru Y, Krimmer DI, Burgess JK, et al. TGF-β enhances deposition of perlecan from COPD airway smooth muscle [J]. Am J Physiol Lung Cell Mol Physiol, 2012, 302(3): L325-333.
[15] Soulez M, Sirois I, Brassard N, et al. Epidermal growth factor and perlecan fragments produced by apoptotic endothelial cells co-ordinately activate ERK1/2-dependent antiapoptotic pathways in mesenchymal stem cells [J]. Stem Cells, 2010, 28(4): 810-820.