光动力疗法对种植体周炎症因子含量的影响

doi:10.19439/j.sjos.2019.01.012

上海口腔医学 ›› 2019, Vol. 28 ›› Issue (1): 63-66.doi: 10.19439/j.sjos.2019.01.012

光动力疗法对种植体周炎症因子含量的影响

周琳怡, 张楚南, 顾迎新, 赖红昌

上海交通大学医学院附属第九人民医院·口腔医学院口腔种植科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海 200011

收稿日期:2018-01-05 修回日期:2018-03-22 出版日期:2019-02-25 发布日期:2019-04-12
通讯作者: 赖红昌,E-mail: lhc9@hotmail.com
作者简介:周琳怡（1992-）,女,硕士,E-mail: zhou_linyi@qq.com

Effect of photodynamic therapy on interleukin in gingival crevicular fluid from patients receiving oral implantation

ZHOU Lin-yi, ZHANG Chu-nan, GU Ying-xin, LAI Hong-chang

Department of Implant Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology. Shanghai 200011, China

Received:2018-01-05 Revised:2018-03-22 Online:2019-02-25 Published:2019-04-12

摘要/Abstract

摘要： 目的:通过检测光动力治疗（photodynamic therapy,PDT）前、后患者龈沟液IL-1β、TNF-α、IL-6和IL-17的含量变化,评价Denfotex光动力治疗系统控制炎症的临床效果。方法:对20例磨牙区单牙种植患者行PDT治疗。选用635 nm,100 mW二极管激光,1 mL 12.7 mg/L甲苯胺蓝(TBO),近、远、颊、舌腭4个位点各照射90 s。应用双抗体夹心酶联免疫吸附法（enzyme linked immunosorbent assay,ELISA）检测治疗前、治疗后2周、3个月龈沟液中IL-1β、TNF-α、IL-6和IL-17的含量,光动力疗法分别于基线、2周采样后重复应用。采用SPSS 23.0软件包对数据进行统计学分析。结果:治疗后2周,龈沟液IL-1β、TNF-α含量与治疗前基线相比均有所降低,但无显著差异;治疗后3个月,4种检测因子较治疗后2周及基线均有显著降低（P<0.05）。结论:Denfotex光动力治疗能显著降低种植患者龈沟液中炎症因子含量,可作为有效预防种植体周围炎的一种治疗方法。

关键词: 口腔种植, 光动力疗法, IL-1β, TNF-α, IL-6, IL-17

Abstract: PURPOSE: Photodynamic therapy (PDT) has been applied in treatment of peri-implantitis for its antimicrobial effects. The aim of the study was to evaluate the effects of a Denfotex PAD Light System's photodynamic therapy on prevention of peri-implantitis. METHODS: Twenty patients with implants in molar area were treated with photoactivated disinfection right after completion of suprastructures. The irradiation time was 30 seconds for mesial, distal, buccal and lingual/palatal sites respectively at a power output of 100mW. Gingival crevicular fluid (GCF) samples from these patients were obtained before photodynamic treatment, 2 weeks and 3 months after treatment. GCF was collected using paper points, and enzyme-linked immunosorbent assay (ELISA) was performed to determine the cytokine (IL-1β, ΤΝF-α, IL-6 and ΙL-17) levels. The data were analyzed with SPSS 23.0 software package. RESULTS: ELISA showed IL-1β and ΤΝF-α levels of all patients were decreased 2 weeks and 3 months after treatment. Reduction of IL-1β, ΤΝF-α, IL-6 and IL-17 from 2 weeks to 3 months and from baseline to 3 months all showed a significant difference (P<0.05). CONCLUSIONS: Denfotex PAD^TM light system is effective in reducing peri-implant inflammatory mediators and can work as an effective treatment to prevent peri-implantitis.

Key words: Peri-implantitis, Photodynamic therapy, IL-1β, TNF-α, IL-6, IL-17

中图分类号:

R783.6

周琳怡, 张楚南, 顾迎新, 赖红昌. 光动力疗法对种植体周炎症因子含量的影响[J]. 上海口腔医学, 2019, 28(1): 63-66.

ZHOU Lin-yi, ZHANG Chu-nan, GU Ying-xin, LAI Hong-chang. Effect of photodynamic therapy on interleukin in gingival crevicular fluid from patients receiving oral implantation[J]. Shanghai Journal of Stomatology, 2019, 28(1): 63-66.

参考文献

[1] Rocha FS, Jesus RN, Rocha FM, et al.Saliva versus peri-implant inflammation: quantification of IL-1beta in partially and totally edentulous patients[J]. J Oral Implantol, 2014, 40(2): 169-173.
[2] Tzach-Nahman R, Mizraji G, Shapira L, et al.Oral infection with Porphyromonas gingivalis induces peri-implantitis in a murine model: Evaluation of bone loss and the local inflammatory response[J]. J Clin Periodontol, 2017, 44(7): 739-748.
[3] Konermann A, Gotz W, Le M, et al.Histopathological verification of osteoimmunological mediators in peri-implantitis and correlation to bone loss and implant functional period[J]. J Oral Implantol, 2016, 42(1): 61-68.
[4] Wang HL, Garaicoa-Pazmino C, Collins A, et al.Protein biomarkers and microbial profiles in peri-implantitis[J]. Clin Oral Implants Res, 2016, 27(9): 1129-1136.
[5] Kikuchi T, Mogi M, Okabe I, et al.Adjunctive application of antimicrobial photodynamic therapy in nonsurgical periodontal treatment: a review of literature[J]. Int J Mol Sci, 2015, 16(10): 24111-24126.
[6] Dörtbudak O, Haas R, Bernhart T.Lethal photosensitization for decontamination of implant surfaces in the treatment of peri-implantitis[J]. Clin Oral Implants Res, 2001, 12(2): 104-108.
[7] Bassetti M, Schär D, Wicki B, et al.Anti-infective therapy of peri-implantitis with adjunctive local drug delivery or photodynamic therapy: 12-month outcomes of a randomized controlled clinical trial[J]. Clin Oral Implants Res, 2014, 25(3): 279-287.
[8] Mizutani K, Aoki A, Coluzzi D, et al.Lasers in minimally invasive periodontal and peri-implant therapy[J]. Periodontol 2000, 2016, 71(1): 185-212.
[9] Duarte PM, Serrao CR, Miranda TS, et al.Could cytokine levels in the peri-implant crevicular fluid be used to distinguish between healthy implants and implants with peri-implantitis? A systematic review[J]. J Periodontal Res, 2016, 51(6): 689-698.
[10] Renvert S, Widén C, Persson GR.Cytokine expression in peri-implant crevicular fluid in relation to bacterial presence[J]. J Clin Periodontol, 2015, 42(7): 697-702.
[11] Salvi GE, Aglietta M, Eick S, et al.Reversibility of experimental peri-implant mucositis compared with experimental gingivitis in humans[J]. Clin Oral Implants Res, 2012, 23(2): 182-190.
[12] Hienz SA, Paliwal S, Ivanovski S.Mechanisms of bone resorption in periodontitis[J]. J Immunol Res, 2015, 2015: 615486.
[13] Kolbe MF, Ribeiro FV, Luchesi VH, et al.Photodynamic therapy during supportive periodontal care: clinical, microbiologic, immunoinflammatory, and patient-centered performance in a split-mouth randomized clinical trial[J]. J Periodontol, 2014, 85(8): e277-286.
[14] Yu JJ, Ruddy MJ, Wong GC, et al.An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals[J]. Blood, 2007, 109(9): 3794-3802.
[15] Lindén A, Laan M, Anderson GP.Neutrophils, interleukin-17A and lung disease[J]. Eur Respir J, 2005, 25(1): 159-172.
[16] Toh ML, Kawashima M, Hot A, et al.Role of IL-17 in the Th1 systemic defects in rheumatoid arthritis through selective IL-12Rbeta2 inhibition[J]. Ann Rheum Dis, 2010, 69(8): 1562-1567.
[17] Ciucci T, Ibáñez L, Boucoiran A, et al.Bone marrow Th17 TNFalpha cells induce osteoclast differentiation, and link bone destruction to IBD[J]. Gut, 2015, 64(7): 1072-1081.
[18] Konttinen YT, Laine P, Kitti U, et al.Immunohistochemical evaluation of inflammatory mediators in failing implants[J]. Int J Periodontics Restorative Dent, 2006, 26(2): 135-141.
[19] Johnson RB, Serio FG.The contribution of interleukin-13 and -15 to the cytokine network within normal and diseased gingiva[J]. J Periodontol, 2007, 78(4): 691-695.
[20] Segarra-Vidal M, Guerra-Ojeda S, Valles LS, et al.Effects of photodynamic therapy in periodontal treatment: A randomized, controlled clinical trial[J]. J Clin Periodontol, 2017, 44(9): 915-925.
[21] Beklen A, Ainola M, Hukkanen M, et al.MMPs, IL-1, and TNF are regulated by IL-17 in periodontitis[J]. J Dent Res, 2007, 86(4): 347-351.
[22] Zoccal KF, Bitencourt Cda S, Sorgi CA, et al.Ts6 and Ts2 from Tityus serrulatus venom induce inflammation by mechanisms dependent on lipid mediators and cytokine production[J]. Toxicon, 2013, 61: 1-10.
[23] Renvert S, Widén C, Persson RG.Cytokine and microbial profiles in relation to the clinical outcome following treatment of peri-implantitis[J]. Clin Oral Implants Res, 2017, 28(9): 1127-1132.
[24] de Moraes M, de Vasconcelos RC, Longo JP, et al. Effects of photodynamic therapy mediated by nanoemulsion containing chloro-aluminum phthalocyanine: a histologic and immunohistochemical study in human gingiva[J]. Photodiagnosis Photodyn Ther, 2015, 12(4): 592-597.

光动力疗法对种植体周炎症因子含量的影响

Effect of photodynamic therapy on interleukin in gingival crevicular fluid from patients receiving oral implantation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	卜晓霜, 王晓, 潘涛华, 朱丽雷, 郭锦材. MB-PDT辅助基础治疗对下前牙牙槽骨角形吸收的临床疗效评价[J]. 上海口腔医学, 2024, 33(3): 265-268.
[2]	范雅丹, 束蓉, 程岚, 葛琳华. 光动力疗法辅助治疗Ⅲ期、Ⅳ期牙周炎的临床效果评价[J]. 上海口腔医学, 2022, 31(5): 501-506.
[3]	曹伟玉, 曹捷, 刘宾益, 徐舟, 陆卫青. 2种种植修复制作工艺的Ti-base基台一体冠用于口腔种植单冠修复精度的研究[J]. 上海口腔医学, 2022, 31(4): 414-417.
[4]	张停停, 胡晓菁, 林璐. 种植体植入15年内种植体周围炎和种植周黏膜炎发生率调查[J]. 上海口腔医学, 2021, 30(3): 292-296.
[5]	林勇, 毛驰, 侯丹, 常晓荣, 张军华, 赵献银. 富自体浓缩生长因子纤维蛋白液联合Bio-Oss骨粉对口腔种植引导性骨再生术后黏膜愈合和骨缺损再生的影响[J]. 上海口腔医学, 2020, 29(3): 316-320.
[6]	常忠福, 姜丹丹, 张志荣, 蔡建英. 口腔种植修复术在牙列缺损患者中的应用效果及对龈沟液中TNF-α、IL-6水平的影响[J]. 上海口腔医学, 2020, 29(2): 217-220.
[7]	董正杰, 徐侃. 种植数字化印模精确度分析[J]. 上海口腔医学, 2020, 29(1): 102-104.
[8]	林哲靖, 罗仁惠, 谢莉莉, 吴晓栩. 牙周基础治疗对龈沟液和血清内皮素、VEGF-A和TNF-α水平的影响[J]. 上海口腔医学, 2019, 28(5): 504-508.
[9]	谭建苹, 蒋倩, 谭明凤, 周鹏, 张福军. 光动力疗法辅助治疗种植体周围炎疗效的meta分析[J]. 上海口腔医学, 2019, 28(3): 330-336.
[10]	王奕月, 林晓萍. Graves病合并牙周炎患者IL-6和TNF-α检测及意义[J]. 上海口腔医学, 2018, 27(1): 43-47.
[11]	陈婉红, 蔡世雄, 孙玉环. 视觉模拟评分法联合疼痛相关因子评估不同正畸力对人活体牙髓的影响[J]. 上海口腔医学, 2017, 26(5): 561-564.
[12]	董正杰, 徐侃. 上颌中切牙长轴与牙槽骨方向夹角的锥形束CT测量分析[J]. 上海口腔医学, 2017, 26(4): 458-460.
[13]	罗蓉, 罗军, 夏罗英. 口腔种植修复在牙列缺失患者中的应用效果及对龈沟液中TNF-α、IL-6、IL-8的影响[J]. 上海口腔医学, 2017, 26(3): 324-327.
[14]	张凌怡, 滕梅荣. 疼痛管理对口腔种植围术期患者的影响[J]. 上海口腔医学, 2017, 26(3): 346-348.
[15]	王贵玲, 于雅琼, 郭佳杰, 仇丽鸿. 转化生长因子β3对脂多糖诱导的成骨细胞中IL-6表达的影响[J]. 上海口腔医学, 2017, 26(1): 21-25.