羧甲基壳聚糖及其复合物对根管内粪肠球菌的作用评价

上海口腔医学 ›› 2013, Vol. 22 ›› Issue (3): 265-269.

羧甲基壳聚糖及其复合物对根管内粪肠球菌的作用评价

宋雪莲¹，孙莹莹²，刘扬¹，陈青¹，毕欣欣¹，于静涛¹

(1.中国医科大学口腔医学院牙体牙髓病科，辽宁口腔医学研究所，沈阳 110002；2.阜新市中心医院口腔科，辽宁阜新 123000)

收稿日期:2012-12-04 修回日期:2013-01-07 出版日期:2013-06-10 发布日期:2013-06-10
通讯作者: 于静涛，Tel：024-22895932，E-mail：yjtao555@sina.com
作者简介:宋雪莲(1986-),女,在读硕士研究生,E-mail:sxl266@163.com
基金资助:
辽宁省重大科技计划项目(2010225001)

An in vitro evaluation of the effect of carboxymethyl chitosan and its composites against Enterococcus faecalis in the root canal

SONG Xue-lian¹, SUN Ying-ying²,LIU Yang¹,CHEN Qing¹,BI Xin-xin¹,YU Jing-tao¹

1.Department of Endodontics, School of Stomatology, China Medical University. Shenyang 110002; 2.Department of Stomatology， Central Hospital of Fuxin City. Fuxin 123000,Liaoning Province，China

Received:2012-12-04 Revised:2013-01-07 Online:2013-06-10 Published:2013-06-10
Supported by:
Supported by Key Science and Technology Project of Liaoning Province(2010225001).

摘要/Abstract

摘要： 目的：探讨羧甲基壳聚糖(carboxymethyl chitosan，CMCS)及其复合物对根管内粪肠球菌的抑菌作用，为在根管治疗方面的应用积累数据。方法：建立粪肠球菌感染根管模型，随机分为A、B、C、D 4组，分别将2%氯己定、140 mg/mL羧甲基壳聚糖氯己定合剂、5 mg/mL羧甲基壳聚糖、Ca(OH)2糊剂注满相应组的各样本根管腔内，培养7 d。封药前、后取样，分别接种在BHI琼脂平皿上，48h后计数菌落形成单位(CFU/mL)。采用SPSS13.0软件包对数据进行统计学分析。结果：封药前，各组根管内细菌量差异无显著性(P>0.05)。封药后即刻，各组根管内细菌量的减少差异显著(P<0.05)。A组与B组的抗菌活性显著优于C组、D组。结论：2%氯己定与140 mg/mL羧甲基壳聚糖氯己定合剂对粪肠球菌具有较强的抗菌活性。

关键词: 粪肠球菌, 羧甲基壳聚糖, 氯己定, 氢氧化钙糊剂

Abstract: PURPOSE: To evaluate the bacteriostasis effect of carboxymethyl chitosan and its composites on intracanal E. faecalis. METHODS: The pattern of E. faecalis infecting root canal was established, and then divided into 4 groups(A, B, C and D).They were filled with 2% chlorhexidine solution, 140 mg/mL mixture of carboxymethyl chitosan and chlorhexidine solution, 5 mg/mL carboxymethyl chitosan solution and calcium hydroxide paste respectively and incubated for 7 days. Samples obtained before and after the intracanal medication were plated onto BHI media to determine the colony-forming units (CFU/mL) after 48 hours. The data were analysed using SPSS 13.0 software package. RESULTS: Before intracanal medication, the variance of bacterial counts were not significantly different (P>0.05). After medication, the four groups showed significant difference in bacterial counts immediately(P<0.05).The antimicrobial effects of A and B group were better than group C and D. CONCLUSIONS: The antibiotic activity of 2% chlorhexidine solution and 140 mg/L mixture of carboxymethyl chitosan and chlorhexidine solution to E.faecalis were better.

Key words: Enterococcus faecalis, Carboxymethyl chitosan, Chlorhexidine;Calcium hydroxide past

中图分类号:

R781.3

宋雪莲, 孙莹莹, 刘扬, 陈青, 毕欣欣, 于静涛. 羧甲基壳聚糖及其复合物对根管内粪肠球菌的作用评价[J]. 上海口腔医学, 2013, 22(3): 265-269.

SONG Xue-lian, SUN Ying-ying, LIU Yang, CHEN Qing, BI Xin-xin, YU Jing-tao. An in vitro evaluation of the effect of carboxymethyl chitosan and its composites against Enterococcus faecalis in the root canal[J]. Shanghai Journal of Stomatology, 2013, 22(3): 265-269.

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