趋化因子CCL21、E-selectins、Hsp90在实验性牙周炎大鼠牙周组织中的表达及意义

doi:10.19439/j.sjos.2020.04.003

上海口腔医学 ›› 2020, Vol. 29 ›› Issue (4): 350-354.doi: 10.19439/j.sjos.2020.04.003

趋化因子CCL21、E-selectins、Hsp90在实验性牙周炎大鼠牙周组织中的表达及意义

王婷, 付静, 刘新强, 张倩

青岛大学附属医院口腔正畸科,山东青岛 266500

收稿日期:2019-11-18 修回日期:2020-01-02 出版日期:2020-08-25 发布日期:2020-09-11
通讯作者: 王婷,E-mail:wting8561@163.com
作者简介:王婷（1985-）,女,硕士,主治医师
基金资助:
国家自然科学基金（51703106）

Expression and significance of chemokines CCL21, E-selectins and Hsp90 in periodontal tissues of rats with experimental periodontitis

WANG Ting, FU Jing, LIU Xin-qiang, ZHANG Qian

Department of Orthodontics, Affiliated Hospital of Qingdao University. Qingdao 266500, Shandong Province, China

Received:2019-11-18 Revised:2020-01-02 Online:2020-08-25 Published:2020-09-11

摘要/Abstract

摘要： 目的：探讨趋化因子CCL21、E选择素（E-selectins）、热休克蛋白90(heat shock protein 90,Hsp90)在实验性牙周炎大鼠牙周组织中的表达及意义。方法：选取40只10周龄雄性Wistar大鼠,随机分为4组,每组10只。A、B、C组均建立牙周炎模型,其余10只为空白对照组。A、B、C组分别于牙周基础治疗后4、8、12周处死并取第一、二磨牙间牙周组织进行CCL21、E-selectins、Hsp90 mRNA 和蛋白表达检测。采用SPSS 25.0软件包对数据进行统计学分析。结果：A、B、C组牙周附着水平显著大于对照组（P<0.05）;牙周基础治疗后,牙周附着水平呈先升高后降低趋势,牙周组织中CCL21、E-selectins、Hsp90 mRNA以及蛋白相对表达量呈降低趋势（P<0.05）。结论：CCL21、E-selectins、Hsp90 mRNA在牙周炎组织中表达上调,随着牙周局部治疗,其表达水平逐渐降低。

关键词: CCL21, E-selectins, Hsp90, 牙周炎

Abstract: PURPOSE: To investigate the expression and significance of chemokines CCL21, E-selectins and heat shock protein 90 (Hsp90) in periodontal tissues of rats with experimental periodontitis. METHODS: Forty 10-week-old male Wistar rats were significantly randomly divided into 4 groups with 10 rats in each group. Periodontitis models were established in groups A, B and C, and the rest were 10 blank control groups. Rats in group A, B and C were sacrificed at 4, 8 and 12 weeks after basic periodontal treatment, and the periodontal tissues of the first and second molars were taken for CCL21, E-selectins and Hsp90 protein expression detection. SPSS 25.0 software package was used to analyze the data. RESULTS: The levels of periodontal attachment in group A, B and C were higher than those in the control group(P< 0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 mRNA and protein expression in periodontal tissues increased first and then decreased(P< 0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 mRNA and protein expression in group B and C were significantly higher than those in group A(P< 0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 and relative protein expression in periodontal tissues of group C were significantly lower than those of group B(P< 0.05). CONCLUSIONS: The expression of CCL21, E-selectins and Hsp90 is up-regulated in periodontitis tissues. With local periodontal treatment, the expression level of CCL21, E-selectins and Hsp90 gradually decreases.

Key words: CCL21, E-selectins, Hsp90, Periodontitis

中图分类号:

R781.4

王婷, 付静, 刘新强, 张倩. 趋化因子CCL21、E-selectins、Hsp90在实验性牙周炎大鼠牙周组织中的表达及意义[J]. 上海口腔医学, 2020, 29(4): 350-354.

WANG Ting, FU Jing, LIU Xin-qiang, ZHANG Qian. Expression and significance of chemokines CCL21, E-selectins and Hsp90 in periodontal tissues of rats with experimental periodontitis[J]. Shanghai Journal of Stomatology, 2020, 29(4): 350-354.

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趋化因子CCL21、E-selectins、Hsp90在实验性牙周炎大鼠牙周组织中的表达及意义

Expression and significance of chemokines CCL21, E-selectins and Hsp90 in periodontal tissues of rats with experimental periodontitis

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